Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 498 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Related products
Overview | ![]() ![]() |
See also: Cloning, DNA Fragmentation, DNA & RNA Markers and Ladders, Gel Electrophoresis, Gene Expression Analysis & Genotyping, Loop-Mediated Isothermal Amplification (LAMP), Coronavirus (SARS-CoV-2), Polymerase Chain Reaction (PCR), Reverse Transcription PCR (RT-PCR), RNA Immunoprecipitation (RIP), RNA Purification & Analysis, Sanger Sequencing, PCR Detection of Viral DNA/RNA
Molecular weight N/A | Excitation (nm) 498 | Emission (nm) 522 |
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid gel stain. It has the same spectral properties to those of SYBR® Green, thus a great replacement to SYBR® Green (SYBR® Green is the trademark of ThermoFisher). It is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Helixyte™ Green has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green stain is ideal for use with laser scanners with the same instrument settings of SYBR Green. Helixyte™ Green is much more sensitive than ethidium bromide for DNA in agarose gels. The gels soaked in diluted Helixyte™ Green stain can be visualized without desalting. It is compatible with UV transilluminators, gel documentation systems, and laser scanners.
Example protocol
AT A GLANCE
Spectral Properties of Helixyte™ Green Nucleic Acid Gel Stain
Excitation/Emission: 497/521 nm when bound to DNA
Important Helixyte™ Green nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Green stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. Dispose of the stain in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommend the gel running time does not exceed more than 2 hours. The following protocols are recommended. However some comparisons might be needed to determine which one better meets your needs.
Excitation/Emission: 497/521 nm when bound to DNA
Important Helixyte™ Green nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Green stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. Dispose of the stain in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommend the gel running time does not exceed more than 2 hours. The following protocols are recommended. However some comparisons might be needed to determine which one better meets your needs.
PREPARATION OF WORKING SOLUTION
Helixyte™ Green working solution (1X)
Make 1X Helixyte™ Green working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0). Note Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.
Note In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.
SAMPLE EXPERIMENTAL PROTOCOL
Post-Staining Protocol
- Run gels based on your standard protocol.
- Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X Helixyte™ Green working solution to submerge the gel.
Note Do not use a glass container, as it will adsorb much of the dye in the staining solution. - Agitate the gel gently at room temperature for ~30 minutes, protected from the light.
Note The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times. - Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
Pre-Casting Protocol
- Prepare agarose gel solution using your standard protocol.
- Add 1X Helixyte™ Green working solution to the gel and mix thoroughly.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
DNA-Staining Before Electrophoresis
- Incubate DNA with a 1:3000 to 1:10,000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.
Product Family
Name | Excitation (nm) | Emission (nm) |
Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution* | 496 | 539 |
Images
Citations
View all 2 citations: Citation Explorer
Directed evolution of Polymerases and its application in Sequence Saturation Mutagenesis
Authors: Chung, Mu-En
Journal: (2021)
Authors: Chung, Mu-En
Journal: (2021)
A promiscuous DNA packaging machine from bacteriophage T4
Authors: Zhang, Zhihong and Kottadiel, Vishal I and Vafabakhsh, Reza and Dai, Li and Chemla, Yann R and Ha, Taekjip and Rao, Venigalla B
Journal: PLoS biology (2011): e1000592
Authors: Zhang, Zhihong and Kottadiel, Vishal I and Vafabakhsh, Reza and Dai, Li and Chemla, Yann R and Ha, Taekjip and Rao, Venigalla B
Journal: PLoS biology (2011): e1000592
References
View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Application notes
Experimental Protocol for DNA Agarose Gel Electrophoresis
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets