Helixyte™ Green Nucleic Acid Gel Stain 10,000X DMSO Solution

160 ng of 1 Kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Helixyte™ Green and SYBR® Green and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Molecular weight

N/A

Excitation (nm)

498

Emission (nm)

522

Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid gel stain. It has the same spectral properties to those of SYBR® Green, thus a great replacement to SYBR® Green (SYBR® Green is the trademark of ThermoFisher). It is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Helixyte™ Green has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green stain is ideal for use with laser scanners with the same instrument settings of SYBR Green. Helixyte™ Green is much more sensitive than ethidium bromide for DNA in agarose gels. The gels soaked in diluted Helixyte™ Green stain can be visualized without desalting. It is compatible with UV transilluminators, gel documentation systems, and laser scanners.

Example protocol

AT A GLANCE

Spectral Properties of Helixyte™ Green Nucleic Acid Gel Stain
Excitation/Emission: 497/521 nm when bound to DNA
Important Helixyte™ Green nucleic acid gel stain is significantly less mutagenic than ethidium bromide. However, we must caution that no data are available on the mutagenicity or toxicity of Helixyte™ Green stain in humans. It should be treated as a potential mutagen and used with appropriate care due to the fact that this reagent binds to nucleic acids. Dispose of the stain in compliance with local regulations.
We have found the greatest sensitivity is achieved by post-staining which also eliminates the possibility of dye interference with DNA migration. While the precast protocol is more convenient, some DNA samples may experience migration, it is highly recommend the gel running time does not exceed more than 2 hours. The following protocols are recommended. However some comparisons might be needed to determine which one better meets your needs.

PREPARATION OF WORKING SOLUTION

Helixyte™ Green working solution (1X)

Make 1X Helixyte™ Green working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0).
Note Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.
Note In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.

SAMPLE EXPERIMENTAL PROTOCOL

Post-Staining Protocol

Run gels based on your standard protocol.
Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X Helixyte™ Green working solution to submerge the gel.
Note Do not use a glass container, as it will adsorb much of the dye in the staining solution.
Agitate the gel gently at room temperature for ~30 minutes, protected from the light.
Note The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times.
Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

Pre-Casting Protocol

Prepare agarose gel solution using your standard protocol.
Add 1X Helixyte™ Green working solution to the gel and mix thoroughly.
Run gels based on your standard protocol.
Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

DNA-Staining Before Electrophoresis

Incubate DNA with a 1:3000 to 1:10,000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
Run gels based on your standard protocol.
Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	498
Emission (nm)	522

Product Family

Name	Excitation (nm)	Emission (nm)
Helixyte™ Gold Nucleic Acid Gel Stain 10,000X DMSO Solution	496	539

Images

Figure 1. 160 ng of 1 Kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Helixyte™ Green and SYBR® Green and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

Citations

View all 2 citations: Citation Explorer

Directed evolution of Polymerases and its application in Sequence Saturation Mutagenesis
Authors: Chung, Mu-En
Journal: (2021)

A promiscuous DNA packaging machine from bacteriophage T4
Authors: Zhang, Zhihong and Kottadiel, Vishal I and Vafabakhsh, Reza and Dai, Li and Chemla, Yann R and Ha, Taekjip and Rao, Venigalla B
Journal: PLoS biology (2011): e1000592

References

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Journal: Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
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Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
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TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
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