HHBS [Hanks' Buffer with 20 mM Hepes]
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| Overview |  SDSProtocol |
See also: Membrane Potential and Channels
Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation such as calcium, membrane potential assay buffer preparation.
Images
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Figure 1. HHBS [Hanks’ Buffer with 20 mM Hepes]
Citations
View all 7 citations: Citation Explorer
Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1--16
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1--16
Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology
Authors: Uslu, Fazil E and Davidson, Christopher D and Mailand, Erik and Bouklas, Nikolaos and Baker, Brendon M and Sakar, Mahmut Selman
Journal: Advanced Materials (2021): 2102641
Authors: Uslu, Fazil E and Davidson, Christopher D and Mailand, Erik and Bouklas, Nikolaos and Baker, Brendon M and Sakar, Mahmut Selman
Journal: Advanced Materials (2021): 2102641
Mypcyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1-16
Authors: Takashima, Shinichiro and Usui, Soichiro and Inoue, Oto and Goten, Chiaki and Yamaguchi, Kosei and Takeda, Yusuke and Cui, Shihe and Sakai, Yoshio and Hayashi, Kenshi and Sakata, Kenji and others,
Journal: Scientific reports (2021): 1-16
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
Journal: BioTechniques (2021): 271--277
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
Journal: BioTechniques (2021): 271--277
In vitro formation of the Merkel cell-neurite complex in embryonic mouse whiskers using organotypic co-cultures
Authors: Ishida, Kentaro and Saito, Tetsuichiro and Mitsui, Toshiyuki
Journal: Development, growth & differentiation (2018)
Authors: Ishida, Kentaro and Saito, Tetsuichiro and Mitsui, Toshiyuki
Journal: Development, growth & differentiation (2018)
Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis
Authors: Pesqueira, Tamagno and Costa-Almeida, Raquel and Gomes, Manuela E
Journal: Scientific reports (2017): 1--12
Authors: Pesqueira, Tamagno and Costa-Almeida, Raquel and Gomes, Manuela E
Journal: Scientific reports (2017): 1--12
Application notes
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How to quantitate ATP induced calcium flux inside mammalian cells using fluorescent calcium indicators