Hoechst 33342 *Ultrapure Grade* *CAS 23491-52-3*

! Interactive Product Finder: This product has alternative forms and/or upgrades. View now


Image Viewer
<strong>Workflow for the “three-in-one” cell death screening assay. </strong>HUVECs growing in the 96-well plate for 48 hours are exposed to NPs for 24 hours. Three types of cell death are evaluated simultaneously. A) Cell necrosis is measured spectrophotometrically after mixing an aliquot of cell supernatant with LDH substrate. B) Cell viability is assessed by adding WST-8 substrate to the cells. After three hours of incubation, aliquots of the reaction mixture are transferred into the new plate and measured spectrophotometrically. C) Cell apoptosis is detected after incubating the cells with Hoechst 33342 and fixing them with paraformaldehyde. Images captured under the inverted fluorescence microscope are computationally processed with the specially designed ImageJ macro. Source: <strong>An effective “three-in-one” screening assay for testing drug and nanoparticle toxicity in human endothelial cells</strong> by Marcela Filipova et al., <em>PLOS</em>, Oct. 2018.
Roll over image to zoom in





Loading...
 
Unit Size: Cat No: Price (USD): Qty:
17530 $95


Export item/cart as Excel file

Send item/cart as email
EXPORT TO EXCEL X

Export:
EXPORT TO EMAIL X
Important: We request your email address to ensure that the recipient(s) knows you intended for them to see the email, and that it is not junk mail.
Export:
Your Name*:
Your Email*:
Recipient Email*:
Your Personal Message:
Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)350/451
MW561.93
CAS #23491-52-3
SolventWater
Storage Freeze (<-15 °C)
Minimize light exposure
Category DNA and RNA,
Biomolecule Detection,
Cell Imaging
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. These dyes can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.




Calculators
Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 33342 *Ultrapure Grade* *CAS 23491-52-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

Sorry, your browser does not support inline SVG. Relative Intensity (%) 100 80 60 40 20 0 Sorry, your browser does not support inline SVG.
Sorry, your browser does not support inline SVG. Sorry, your browser does not support inline SVG.
Move mouse over grid to display wavelength & intensity values.

300
400
500
600
700
800
900
Wavelength (nm)





Protocol


Quick Preview

This protocol only provides a guideline, and should be modified according to your specific needs.

The following procedure can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and other factors may influence staining. Residual detergent on glassware may also affect real or apparent staining of many organisms, causing brightly stained material to appear in solutions with or without cells present.

Pellet cells by centrifugation and resuspend the cells in buffered salt solutions or media, with optimal dye binding at pH 7.4. Adherent cells in culture may be stained in situ on cover slips or in the cell culture wells. Add Hoechst stain using the concentrations between 0.5 and 5 µM and incubate it for 15 to 60 minutes as a guide. In initial experiments, it may be best to try several dye concentrations over the entire suggested range to determine the concentration that yields optimal staining.






Interactive Product Finder

Alternative forms
Product nameCurrent ProductRelated Product
Hoechst 33342 *20 mM solution in water*
Ultrapure Grade CAS 23491 52 3
20 mM solution in water










References

An effective “three-in-one” screening assay for testing drug and nanoparticle toxicity in human endothelial cells
Authors: Marcela Filipova, Oumsalama K Elhelu, Silvia H De Paoli, Zuzana Fremuntova, Tibor Mosko, Dusan Cmarko, Jan Simak, Karel Holada
Journal: PloS one (2018): e0206557

Cell electrofusion to improve efficacy and thermotolerance of the entomopathogenic fungus, Beauveria bassiana
Authors: Agrin Davari, Margaret Skinner, Bruce L Parker
Journal: Journal of applied microbiology (2018)

Polyethylene glycol and octaarginine n dual-functionalized nano-graphene oxide: An optimization for efficient nucleic acids delivery
Authors: Rana Imani, Satya Prakash, Hojatollah Vali, Shahab Faghihi
Journal: Biomaterials Science (2018)

Quanyuan Wan1, Chunrong Yang2, Youliang Rao1, Zhiwei Liao1 and Jianguo Su1
Authors: Uday Kishore, Yunhao Tan, Nicola Tamassia, Jianguo Su, Q Wan, C Yang, Y Rao, Z Liao
Journal: Macromolecular Structure Underlying Recognition in Innate Immunity (2018): 12135

Teleost-Specific TLR19 Localizes to Endosome, Recognizes dsRNA, Recruits TRIF, Triggers both IFN and NF-$\kappa$B Pathways, and Protects Cells from Grass Carp Reovirus Infection
Authors: Jianfei Ji, Youliang Rao, Quanyuan Wan, Zhiwei Liao, Jianguo Su
Journal: The Journal of Immunology (2018): 573--585

Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Joline Tung, Jing Yuan Ching, Yoke Mooi Ng, Lih Shin Tew, Yit Lung Khung
Journal: ACS Applied Materials & Interfaces (2017)

MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
Authors: Quanyuan Wan, Chunrong Yang, Youliang Rao, Zhiwei Liao, Jianguo Su
Journal: Frontiers in Immunology (2017)

Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Rana Imani, Wei Shao, Samira Taherkhani, Shahriar Hojjati Emami, Satya Prakash, Shahab Faghihi
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325

A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: John M Lindner, Christina SF Wong, Andreas Möller, Peter J Nielsen
Journal: Molecular and cellular biology (2013): 4628--4640