IDA maleimide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 369.37 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
IDA succinimidyl ester |
Overview | SDSProtocol |
See also: Affinity Purification
Molecular weight 369.37 |
Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) are two such ligands commonly used in commercially available resins. AAT Bioquest offer a variety of NTA building blocks for developing either NTA-based purification and detection. IDA is complimentary to NTA. The tridentate IDA ligand requires a lower imidazole concentration to elute protein than the tetradentate NTA. IDA is a smaller molecule which can be coupled to the matrix at a higher density resulting in a higher metal loading capacity. IDA maleimide is an excellent building block that can be used to develop a variety of IDA probes for detecting polyHis-containing proteins, and tools for purifying polyHis tagged proteins.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of IDA maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 270.731 µL | 1.354 mL | 2.707 mL | 13.537 mL | 27.073 mL |
5 mM | 54.146 µL | 270.731 µL | 541.462 µL | 2.707 mL | 5.415 mL |
10 mM | 27.073 µL | 135.366 µL | 270.731 µL | 1.354 mL | 2.707 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 28 references: Citation Explorer
Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Production of a truncated recombinant HA1 for influenza A H9 subtype screening.
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555
Characterization of the Eimeria maxima sporozoite surface protein IMP1.
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52
Effects of cholesterol incorporation on the physicochemical, colloidal, and biological characteristics of pH-sensitive AB₂ miktoarm polymer-based polymersomes.
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37
Layer-by-layer polyelectrolyte deposition: a mechanism for forming biocomposite materials.
Authors: Tan, Yerpeng and Yildiz, Umit Hakan and Wei, Wei and Waite, J Herbert and Miserez, Ali
Journal: Biomacromolecules (2013): 1715-26
Authors: Tan, Yerpeng and Yildiz, Umit Hakan and Wei, Wei and Waite, J Herbert and Miserez, Ali
Journal: Biomacromolecules (2013): 1715-26
Construction of the coding sequence of the transcription variant 2 of the human Renalase gene and its expression in the prokaryotic system.
Authors: Fedchenko, Valerii I and Kaloshin, Alexei A and Mezhevikina, Lyudmila M and Buneeva, Olga A and Medvedev, Alexei E
Journal: International journal of molecular sciences (2013): 12764-79
Authors: Fedchenko, Valerii I and Kaloshin, Alexei A and Mezhevikina, Lyudmila M and Buneeva, Olga A and Medvedev, Alexei E
Journal: International journal of molecular sciences (2013): 12764-79
Controlled delivery of recombinant adeno-associated virus serotype 2 using pH-sensitive poly(ethylene glycol)-poly-L-histidine hydrogels.
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45
Detection of biological macromolecules on a biochip dedicated to UV specific absorption.
Authors: Robin, Kristelle and Reverchon, Jean-Luc and Mugherli, Laurent and Fromant, Michel and Plateau, Pierre and Benisty, Henri
Journal: Biosensors & bioelectronics (2009): 1585-91
Authors: Robin, Kristelle and Reverchon, Jean-Luc and Mugherli, Laurent and Fromant, Michel and Plateau, Pierre and Benisty, Henri
Journal: Biosensors & bioelectronics (2009): 1585-91
[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44