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iFluor™ 820-streptavidin conjugate

AAT Bioquest's bright and photostable iFluor™ dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor™ dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
AAT Bioquest's bright and photostable iFluor™ dyes cover the entire spectrum of UV, Vis and IR from 200 to 900 nm. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 647 and 860 nm). iFluor™ dye-labeled streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
Ordering information
Price ()
Catalog Number16977
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight52000
SolventWater
Spectral properties
Correction Factor (260 nm)0.11
Correction Factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)822
Emission (nm)850
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (43)

OverviewpdfSDSpdfProtocol


Molecular weight
52000
Correction Factor (260 nm)
0.11
Correction Factor (280 nm)
0.16
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
822
Emission (nm)
850
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. A variety of the complementary biotinylated reagents are available from numerous commercial vendors. This iFluor™ 820-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor™ 820 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor™ 820-streptavidin conjugates has fluorescence excitation and emission maxima of ~820 nm and ~849 nm respectively. These spectral characteristics make it an excellent alternative to IRDye® 800CW (IRDye® 800CW is the trademark of Li-COR) due to its much longer excitation wavelength (making it much more tissue-penetrating than IRDye® 800CW). It is a very valuable tool for biotin-streptavidin-based biological assays and tests.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of iFluor™ 820-streptavidin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM1.923 µL9.615 µL19.231 µL96.154 µL192.308 µL
5 mM384.615 nL1.923 µL3.846 µL19.231 µL38.462 µL
10 mM192.308 nL961.538 nL1.923 µL9.615 µL19.231 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.11
Correction Factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)822
Emission (nm)850

Citations


View all 15 citations: Citation Explorer
Aptamer Structure Switch Fluorescence Anisotropy Assay for Small Molecules Using Streptavidin as an Effective Signal Amplifier Based on Proximity Effect
Authors: Li, Y., Zhao, Q.
Journal: Anal Chem (2019): 7379-7384
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells
Authors: Goujon, A., Strakova, K., Sakai, N., Matile, S.
Journal: Chem Sci (2019): 310-319
Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection
Authors: Kurdekar, A. D., Avinash Chunduri, L. A., Manohar, C. S., Haleyurgirisetty, M. K., Hewlett, I. K., Venkataramaniah, K.
Journal: Sci Adv (2018): eaar6280
Design and Fabrication of Streptavidin-Functionalized, Fluorescently Labeled Polymeric Nanocarriers
Authors: Jo, A., Zhang, R., Allen, I. C., Riffle, J. S., Davis, R. M.
Journal: Langmuir (2018): 15783-15794
Optimized Streptavidin for Fluorescent Labeling of Biotinylated Targets
Authors: Hytonen, V. P.
Journal: Cell Chem Biol (2017): 921-922
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Streptavidin-triggered signal amplified fluorescence polarization for analysis of DNA-protein interactions
Authors: Choi, J. W., Jo, B. G., deMello, A. J., Choo, J., Kim, H. Y.
Journal: Analyst (2016): 6499-6502
Novel biosensor system model based on fluorescence quenching by a fluorescent streptavidin and carbazole-labeled biotin
Authors: Zhu, X., Shinohara, H., Miyatake, R., Hohsaka, T.
Journal: J Mol Recognit (2016): 485-91
Discrimination between streptavidin and avidin with fluorescent affinity-based probes
Authors: Sun, Q., Tian, H., Qu, H., Sun, D., Chen, Z., Duan, L., Zhang, W., Qian, J.
Journal: Analyst (2015): 4648-53

References


View all 1 references: Citation Explorer
Aluminum phthalocyanine-streptavidin: new, sensitive fluorescent tracer for immunoassay
Authors: Kelly TA, Hunter CA, Schindele DC, Pepich BV.
Journal: Clin Chem (1991): 1283