Indo-1, pentasodium salt

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Physical properties

Dissociation constant (K_d, nM)	230
Molecular weight	759.52
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	33000
Excitation (nm)	330
Emission (nm)	404

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

759.52

Dissociation constant (K_d, nM)

230

Extinction coefficient (cm ^-1 M ^-1)

33000

Excitation (nm)

330

Emission (nm)

404

Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding to Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. This cell-impermeant Indo-1 pentasodium salt is a UV light excitable, emission ratioable Ca2+ indicator. Upon binding to Ca2+, the emission maximum of Indo-1 shifts from 480 nm to 400 nm. Indo-1 is preferred for flow cytometry, in which it is more practical to use a single laser for excitation, such as the 351-364 nm spectral lines of the argon-ion laser.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Indo-1, pentasodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	131.662 µL	658.311 µL	1.317 mL	6.583 mL	13.166 mL
5 mM	26.332 µL	131.662 µL	263.324 µL	1.317 mL	2.633 mL
10 mM	13.166 µL	65.831 µL	131.662 µL	658.311 µL	1.317 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	33000
Excitation (nm)	330
Emission (nm)	404

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Indo-1, pentapotassium salt CAS 132319-56-3	330	404	33000

Images

Figure 1. The fluorescence emission spectra of Indo-1 measured in solutions containing 0 to 39 µM of free calcium ions.

Figure 2. Chemical structure for Indo-1, pentasodium salt

Citations

View all 4 citations: Citation Explorer

Nifedipine stimulates proliferation and migration of different breast cancer cells by distinct pathways
Authors: Zhao, Tao and Guo, Dongqing and Gu, Yuchun and Ling, Yang
Journal: Molecular Medicine Reports (2017): 2259--2263

Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to the development of pulmonary arterial hypertension
Authors: Guo, Dongqing and Gu, Junzhong and Jiang, Hui and Ahmed, Asif and Zhang, Zhiren and Gu, Yuchun
Journal: Journal of molecular and cellular cardiology (2016): 179--187

Nifedipine promotes the proliferation and migration of breast cancer cells
Authors: Guo, Dong-Qing and Zhang, Hao and Tan, Sheng-Jiang and Gu, Yu-Chun
Journal: PloS one (2014): e113649

Bioanalytics/Illumination: Just-right light-Inherently adaptive for application-specific needs
Authors: JOHNSON, IAIN

References

View all 84 references: Citation Explorer

Measurement of [Ca2+] in cell suspensions using indo-1
Authors: Nelemans A., undefined
Journal: Methods Mol Biol (2006): 47

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220

Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence
Authors: Eerbeek O, Mik EG, Zuurbier CJ, van 't Loo M, Donkersloot C, Ince C.
Journal: J Appl Physiol (2004): 2042

Negative inotropic effects of angiotensin II, endothelin-1 and phenylephrine in indo-1 loaded adult mouse ventricular myocytes
Authors: Sakurai K, Norota I, Tanaka H, Kubota I, Tomoike H, Endo M.
Journal: Life Sci (2002): 1173

Usefulness of the analytic method of intracellular calcium and the problems--aequorin and indo-1 signal
Authors: Endoh M., undefined
Journal: Nippon Yakurigaku Zasshi (2000): 361

Comment on "Usefulness of intracellular calcium analysis and the problem--aequorin and indo-1 signal"
Authors: Imaizumi Y., undefined
Journal: Nippon Yakurigaku Zasshi (2000): 101

Concentrations of caffeine greater than 20 mM increase the indo-1 fluorescence ratio in a Ca(2+)-independent manner
Authors: McKemy DD, Welch W, Airey JA, Sutko JL.
Journal: Cell Calcium (2000): 117

Intracellular calcium signals measured with indo-1 in isolated skeletal muscle fibres from control and mdx mice
Authors: Collet C, Allard B, Tourneur Y, Jacquemond V.
Journal: J Physiol (1999): 417

Measurement of [Ca2+]i in cell suspensions using indo-1
Authors: Nelemans A., undefined
Journal: Methods Mol Biol (1999): 41

Alpha-stat calibration of indo-1 fluorescence and measurement of intracellular free calcium in rat ventricular cells at different temperatures
Authors: Wang SQ, Zhou ZQ.
Journal: Life Sci (1999): 871