Indocyanine Green CAS 3599-32-4

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Physical properties

Molecular weight	774.96
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.076
Excitation (nm)	789
Emission (nm)	814

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

3599-32-4

Molecular weight

774.96

Correction Factor (280 nm)

0.076

Excitation (nm)

789

Emission (nm)

814

Indocyanine green (ICG) is a cyanine dye used in medical diagnostics. ICG is used for determining cardiac output, hepatic function, liver blood flow, and for ophthalmic angiography. ICG has a peak spectral absorption close to 800 nm. These infrared frequencies penetrate retinal layers, allowing ICG angiography to image deeper patterns of circulation than fluorescein angiography. ICG binds tightly to plasma proteins and becomes confined to the vascular system. ICG has a half-life of 150 to 180 seconds and is removed from circulation exclusively by the liver to bile juice. A recent study indicated ICG targets atheromas within 20 min of injection and provides sufficient signal enhancement for in vivo detection of lipid-rich, inflamed, coronary-sized plaques in atherosclerotic rabbits. Ex vivo fluorescence reflectance imaging showed high plaque target-to-background ratios in atheroma-bearing rabbits injected with ICG compared to atheroma-bearing rabbits injected with saline.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Indocyanine Green *CAS 3599-32-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	129.039 µL	645.195 µL	1.29 mL	6.452 mL	12.904 mL
5 mM	25.808 µL	129.039 µL	258.078 µL	1.29 mL	2.581 mL
10 mM	12.904 µL	64.519 µL	129.039 µL	645.195 µL	1.29 mL

Molarity calculator

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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.076
Excitation (nm)	789
Emission (nm)	814

Product Family

Name	Excitation (nm)	Emission (nm)
Helixyte™ Green 20X Aqueous PCR Solution	498	522
Helixyte™ Green 10,000X Aqueous PCR Solution	498	522
Thiolite™ Green	510	525
CytoTell™ Green	510	525
LysoBrite™ Green	501	510
Q4ever™ Green 1250X DMSO Solution	503	527

Images

Figure 1. Chemical structure for Indocyanine Green *CAS 3599-32-4*

Citations

View all 1 citations: Citation Explorer

Nanodiamonds for bioapplications, recent developments
Authors: Bondon, Nicolas and Raehm, Laurence and Charnay, Clarence and Boukherroub, Rabah and Durand, Jean-Olivier
Journal: Journal of Materials Chemistry B (2020): 10878--10896

References

View all 37 references: Citation Explorer

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Journal: Gastrointest Endosc (2009): 959

Single-molecule fluorescence spectroelectrochemistry of cresyl violet
Authors: Lei C, Hu D, Ackerman EJ.
Journal: Chem Commun (Camb) (2008): 5490

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Journal: Spectrochim Acta A Mol Biomol Spectrosc (2007): 717

Chromoendoscopy in Barrett's oesophagus: is cresyl violet the magic bullet
Authors: Kiesslich R, Neurath MF.
Journal: Dig Liver Dis (2006): 301

Infarct volume quantification in mouse focal cerebral ischemia: a comparison of triphenyltetrazolium chloride and cresyl violet staining techniques
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Journal: J Neurosci Methods (2004): 203

Cresyl violet as a fluorophore in confocal laser scanning microscopy for future in-vivo histopathology
Authors: George M, Meining A.
Journal: Endoscopy (2003): 585

Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
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Journal: J Histochem Cytochem (2003): 959

DEVDase detection in intact apoptotic cells using the cell permeant fluorogenic substrate, (z-DEVD)2-cresyl violet
Authors: Lee BW, Johnson GL, Hed SA, Darzynkiewicz Z, Talhouk JW, Mehrotra S.
Journal: Biotechniques (2003): 1080

Ala-Pro-cresyl violet, a synthetic fluorogenic substrate for the analysis of kinetic parameters of dipeptidyl peptidase IV (CD26) in individual living rat hepatocytes
Authors: Van Noorden CJ, Boonacker E, Bissell ER, Meijer AJ, van Marle J, Smith RE.
Journal: Anal Biochem (1997): 71

Cresyl violet: a red fluorescent Nissl stain
Authors: Alvarez-Buylla A, Ling CY, Kirn JR.
Journal: J Neurosci Methods (1990): 129