Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. Sanger sequencing results in the formation of extension products of various lengths terminated with dideoxynucleotides at the 3′ end. The extension products are then separated by Capillary Electrophoresis. The molecules are injected by an electrical current into a long glass capillary filled with a gel polymer. The four distinct fluorescent ddNTPs (labeled with BigDye®, BigDye® is the trademark of ThermoFisher) are the critical components for performing Sanger sequencing. MagaDye™ 4 Color Sanger Sequencing Terminator Kit provides four distinct fluorescent ddNTPs that emit 4 different fluorescence colors when illuminated by 488 nm laser beam. The four MagaDye™ fluorescent ddNTP terminators have almost identical spectra to the four BigDye ddNTPs used in Sanger sequencing. Each ddTNP is provided at 5 nmoles.