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MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester]

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Physical properties
Molecular weight331.28
Spectral properties
Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
7-Methoxycoumarin-4-acetic acid (MCA) is widely used for developing FRET peptide substrates for analyzing protease activities. It is commonly paired with dinitrophenyl (DNP) group. It is also used for HPLC derivatization by fluorescence detection. MCA succinimidyl ester selectively reacts with aliphatic amines.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM301.859 µL1.509 mL3.019 mL15.093 mL30.186 mL
5 mM60.372 µL301.859 µL603.719 µL3.019 mL6.037 mL
10 mM30.186 µL150.93 µL301.859 µL1.509 mL3.019 mL

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Spectral properties

Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381



View all 6 references: Citation Explorer
Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306
Fluorescent labeling of peptides on solid phase
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582
Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470
Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Analysis of 5-fluorouracil in plasma by precolumn derivatization with 4-bromomethyl-7-methoxycoumarin, followed by multi-dimensional high-performance liquid chromatography
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431