MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester]

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Physical properties

Molecular weight	331.28
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.3
Excitation (nm)	322
Emission (nm)	381

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

331.28

Correction Factor (280 nm)

0.3

Excitation (nm)

322

Emission (nm)

381

7-Methoxycoumarin-4-acetic acid (MCA) is widely used for developing FRET peptide substrates for analyzing protease activities. It is commonly paired with dinitrophenyl (DNP) group. It is also used for HPLC derivatization by fluorescence detection. MCA succinimidyl ester selectively reacts with aliphatic amines.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	301.859 µL	1.509 mL	3.019 mL	15.093 mL	30.186 mL
5 mM	60.372 µL	301.859 µL	603.719 µL	3.019 mL	6.037 mL
10 mM	30.186 µL	150.93 µL	301.859 µL	1.509 mL	3.019 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.3
Excitation (nm)	322
Emission (nm)	381

Images

Figure 1. Chemical structure for MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester]

References

View all 6 references: Citation Explorer

Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306

Fluorescent labeling of peptides on solid phase
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582

Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470

Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131

Analysis of 5-fluorouracil in plasma by precolumn derivatization with 4-bromomethyl-7-methoxycoumarin, followed by multi-dimensional high-performance liquid chromatography
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431