MeO-Succ-Arg-Pro-Tyr-AMC

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Physical properties

Molecular weight	819.78
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

819.78

Excitation (nm)

341

Emission (nm)

441

MeO-Succ-Arg-Pro-Tyr-AMC is a sensitive fluorogenic substrate for chymotrypsin-like proteases. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of stratum corneum chymotryptic enzyme (SCCE).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MeO-Succ-Arg-Pro-Tyr-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.984 µL	609.92 µL	1.22 mL	6.099 mL	12.198 mL
5 mM	24.397 µL	121.984 µL	243.968 µL	1.22 mL	2.44 mL
10 mM	12.198 µL	60.992 µL	121.984 µL	609.92 µL	1.22 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	341
Emission (nm)	441

Images

Figure 1. Chemical structure for MeO-Succ-Arg-Pro-Tyr-AMC

Citations

View all 1 citations: Citation Explorer

Carbon dioxide ameliorates reduced desquamation in dry scaly skin via protease activation
Authors: Fukagawa, Satoko and Takahashi, Ayami and Sayama, Keimon and Mori, Shinobu and Murase, Takatoshi
Journal: International Journal of Cosmetic Science (2020)

References

View all 101 references: Citation Explorer

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

Amyloidogenic potential of alpha-chymotrypsin in different conformational states
Authors: Rezaei-Ghaleh N, Zweckstetter M, Morshedi D, Ebrahim-Habibi A, Nemat-Gorgani M.
Journal: Biopolymers (2009): 28