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Physical properties
Molecular weight819.78
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins, Proteases
Molecular weight
Excitation (nm)
Emission (nm)
MeO-Succ-Arg-Pro-Tyr-AMC is a sensitive fluorogenic substrate for chymotrypsin-like proteases. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of stratum corneum chymotryptic enzyme (SCCE).


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MeO-Succ-Arg-Pro-Tyr-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM121.984 µL609.92 µL1.22 mL6.099 mL12.198 mL
5 mM24.397 µL121.984 µL243.968 µL1.22 mL2.44 mL
10 mM12.198 µL60.992 µL121.984 µL609.92 µL1.22 mL

Molarity calculator

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Spectral properties

Excitation (nm)341
Emission (nm)441



View all 1 citations: Citation Explorer
Carbon dioxide ameliorates reduced desquamation in dry scaly skin via protease activation
Authors: Fukagawa, Satoko and Takahashi, Ayami and Sayama, Keimon and Mori, Shinobu and Murase, Takatoshi
Journal: International Journal of Cosmetic Science (2020)


View all 101 references: Citation Explorer
A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
Amyloidogenic potential of alpha-chymotrypsin in different conformational states
Authors: Rezaei-Ghaleh N, Zweckstetter M, Morshedi D, Ebrahim-Habibi A, Nemat-Gorgani M.
Journal: Biopolymers (2009): 28