mFluor™ Violet 510 SE

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<p>Signal-to-noise ratio of flow cytometric analysis of CD45 expression on human lymphocytes. Whole blood was stained with Mouse Anti-Human CD45 conjugated with mFluor™ Violet 510 (Cat#1151) or Pacific Orange with different dye-to-protein ratio (D/P). mFluor™ Violet 510 offers improved brightness over Pacific Orange.</p>
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Unit Size: Cat No: Price (USD): Qty:
1 mg 1151 $195


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)414/508
MW770.83
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Superior Labeling Dyes
mFluor Dyes and Kits
Related General proteins
Labeling via Amino Groups
Secondary Reagents
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 510 dyes have fluorescence excitation and emission maxima of ~405 nm and ~510 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Orange™ labeling dye. mFluor™ Violet 510 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 510 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 510 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

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References & Citations

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: D Gao, N Liu, Y Li, Y Zhang, G Liu
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Kenneth Wu, Robert A Chong, Qing Yu, Jin Bai, Donald E Spratt, Kevin Ching, Chan Lee, Haibin Miao, Inger Tappin, Jerard Hurwitz
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Helene Piccard, Nele Berghmans, Eva Korpos, Chris Dillen, Ilse Van Aelst, Sandra Li, Erik Martens, Sandra Liekens, Sam Noppen, Jo Van Damme
Journal: International Journal of Cancer (2012): E425--E436






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Fluorescent Labeling Probes & Kits

Certificate of Analysis