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MMP Red™ substrate

The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.
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Physical properties
Molecular weight~2000
SolventDMSO
Spectral properties
Excitation (nm)545
Emission (nm)572
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Alternative formats
MMP Green™ substrate

OverviewpdfSDSpdfProtocol


See also: Proteases
Molecular weight
~2000
Excitation (nm)
545
Emission (nm)
572
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This FRET substrate is designed to monitor the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. This FRET substrate is based on our TF3/TQ3 FRET pair. Upon MMP hydrolysis the fluorescence of MMP Red™ FRET peptide substrate is increased since the TF3/TQ3 FRET pair is separated. The fluorescence increase is proportional to the MMP enzyme activities.

Spectrum


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spectrum

Spectral properties

Excitation (nm)545
Emission (nm)572

Product Family


NameExcitation (nm)Emission (nm)
MMP Green™ substrate494515

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Citations


View all 10 citations: Citation Explorer
Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3&beta;/snail-mediated pathway
Authors: Xiao, Ruyue and Yuan, Lan and He, Weijiang and Yang, Xiaoda
Journal: Metallomics (2018)
Connexin 43 Upregulation by Dioscin Inhibits Melanoma Progression via Suppressing Malignancy and Inducing M1 Polarization
Authors: Kou, Yu and Ji, Liyan and Wang, Haojia and Wang, Wensheng and Zheng, Hongming and Zou, Juan and Liu, Linxin and Qi, Xiaoxiao and Liu, Zhongqiu and Du, Biaoyan and others, undefined
Journal: International Journal of Cancer (2017)
DACT2, an epigenetic stimulator, exerts dual efficacy for colorectal cancer prevention and treatment
Authors: Lu, Linlin and Wang, Ying and Ou, Rilan and Feng, Qian and Ji, Liyan and Zheng, Hongming and Guo, Yue and Qi, Xiaoxiao and Kong, Ah-Ng Tony and Liu, Zhongqiu
Journal: Pharmacological research (2017)
Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay
Authors: Kittur, Harsha and Tay, Andy and Hua, Avery and Yu, Min and Di Carlo, Dino
Journal: Biophysical Journal (2017): 1858--1867
Inhibition of BET bromodomain attenuates angiotensin II induced abdominal aortic aneurysm in ApoE-/- mice
Authors: Duan, Qiong and Mao, Xiaoxiao and Liao, Chaonan and Zhou, Haoyang and Sun, Zelin and Deng, Xu and Hu, Qiuning and Qi, Jun and Zhang, Guogang and Huang, He and others, undefined
Journal: International Journal of Cardiology (2016): 428--432
Tissue inhibitor of metalloproteinase 1 influences vascular adaptations to chronic alterations in blood flow
Authors: M, undefined and el, Erin R and Uchida, Cass and ra , undefined and Nwadozi, Emmanuel and Makki, Armin and Haas, Tara L
Journal: Journal of Cellular Physiology (2016)
Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
Authors: Przybyla, Laralynne M and Theunissen, Thorold W and Jaenisch, Rudolf and Voldman, Joel
Journal: Stem Cells (2013): 1097--1106
&beta;-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
Authors: Moretti, Silvia and Massi, Daniela and Farini, Valentina and Baroni, Gianna and Parri, Matteo and Innocenti, Stefania and Cecchi, Roberto and Chiarugi, Paola
Journal: Laboratory Investigation (2013): 279--290
Identification and functional validation of CDH11, PCSK6 and SH3GL3 as novel glioma invasion-associated candidate genes
Authors: Delic, S and Lottmann, N and Jetschke, K and Reifenberger, G and Riemenschneider, Markus J
Journal: Neuropathology and applied neurobiology (2012): 201--212
EphA2 induces metastatic growth regulating amoeboid motility and clonogenic potential in prostate carcinoma cells
Authors: Taddei, Maria Letizia and Parri, Matteo and Angelucci, Adriano and Bianchini, Francesca and Marconi, Chiara and Giannoni, Elisa and Raugei, Giovanni and Bologna, Mauro and Calorini, Lido and Chiarugi, Paola
Journal: Molecular Cancer Research (2011): 149--160