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MycoLight™ Green JJ98

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Physical properties
Molecular weight534
SolventDMSO
Spectral properties
Excitation (nm)482
Emission (nm)512
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
534
Excitation (nm)
482
Emission (nm)
512
MycoLight™ Green JJ98 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes. MycoLight™ Green JJ98 stain has a high affinity for DNA and exhibits enhanced fluorescence upon binding with an excitation maximum close to the 488 nm argon laser line and fluorescence emission maximum at ~500 nm. MycoLight™ Green JJ98 stain is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead Gram-positive and Gram-negative bacteria. It is an excellent replacement for SYTO® 9 (SYTO® is the trademark of Invitrogen).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MycoLight™ Green JJ98 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM187.266 µL936.33 µL1.873 mL9.363 mL18.727 mL
5 mM37.453 µL187.266 µL374.532 µL1.873 mL3.745 mL
10 mM18.727 µL93.633 µL187.266 µL936.33 µL1.873 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)482
Emission (nm)512

Product Family


NameExcitation (nm)Emission (nm)
MycoLight™ Green JJ99 *5 mM in DMSO*482512