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Nile Blue A *CAS 3625-57-8*

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Physical properties
Molecular weight732.85
Spectral properties
Absorbance (nm)634
Extinction coefficient (cm -1 M -1)768001
Excitation (nm)631
Emission (nm)660
Quantum yield0.27001
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Nile Blue A is certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Blue A *CAS 3625-57-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM136.454 µL682.268 µL1.365 mL6.823 mL13.645 mL
5 mM27.291 µL136.454 µL272.907 µL1.365 mL2.729 mL
10 mM13.645 µL68.227 µL136.454 µL682.268 µL1.365 mL

Molarity calculator

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Spectral properties

Absorbance (nm)634
Extinction coefficient (cm -1 M -1)768001
Excitation (nm)631
Emission (nm)660
Quantum yield0.27001



View all 34 references: Citation Explorer
Spectroscopic characterization of poly(amidoamine) dendrimers as selective uptake devices: Phenol blue versus Nile red
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
West Nile virus detection in the organs of naturally infected blue jays (Cyanocitta cristata)
Authors: Gibbs SE, Ellis AE, Mead DG, Allison AB, Moulton JK, Howerth EW, Stallknecht DE.
Journal: J Wildl Dis (2005): 354
Probing correlated spectral motion: two-color photon echo study of Nile blue
Authors: Prall BS, Parkinson DY, Fleming GR.
Journal: J Chem Phys (2005): 54515
Methylene blue photoinactivation abolishes West Nile virus infectivity in vivo
Authors: Papin JF, Floyd RA, Dittmer DP.
Journal: Antiviral Res (2005): 84
Thermal cycling and the optical and electrical characterization of self-assembled multilayer nile blue A-gold thin films
Authors: Geist B, Spillman WB, Jr., Claus RO.
Journal: Appl Opt (2005): 6357
Experimental West Nile virus infection in blue jays (Cyanocitta cristata) and crows (Corvus brachyrhynchos)
Authors: Weingartl HM, Neufeld JL, Copps J, Marszal P.
Journal: Vet Pathol (2004): 362
Nile Blue in Triton-X 100/benzene-hexane reverse micelles: a fluorescence spectroscopic study
Authors: Das K, Jain B, Patel HS.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2004): 2059
West Nile virus in plasma is highly sensitive to methylene blue-light treatment
Authors: Mohr H, Knuver-Hopf J, Gravemann U, Redecker-Klein A, Muller TH.
Journal: Transfusion (2004): 886
Patterns of West Nile virus infection in Ohio blue jays: implications for initiation of the annual cycle
Authors: Garvin MC, Tarvin KA, Smith J, Ohajuruka OA, Grimes S.
Journal: Am J Trop Med Hyg (2004): 566
Spectrofluorimetric method for the estimation of total lipids in Eremothecium ashbyii fungal filaments using Nile blue and avoiding interference of autofluorescent riboflavin
Authors: Vijayalakshmi S, Karthika TN, Mishra AK, Ch and ra TS., undefined
Journal: J Microbiol Methods (2003): 99