Nuclear Green™ LCS1 5 mM DMSO Solution

Fluorescence images of oleic acid treated HeLa cells stained with Cell Navigator® Lipid Droplets Fluorescence Assay Kit (Cat#22735) and Nuclear Green™ LCS1 (Cat#17540).

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	503
Emission (nm)	527

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Nuclear Orange™ LCS1 *5 mM DMSO Solution*

Nuclear Red™ LCS1 *5 mM DMSO Solution*

Overview SDS Protocol

Molecular weight

N/A

Excitation (nm)

503

Emission (nm)

527

Our Nuclear Green™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Green™ LCS1 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets.

Spectrum

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Spectral properties

Excitation (nm)	503
Emission (nm)	527

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Orange™ LCS1 5 mM DMSO Solution	514	556
Nuclear Red™ LCS1 5 mM DMSO Solution	622	645

Images

Figure 1. Fluorescence images of oleic acid treated HeLa cells stained with Cell Navigator® Lipid Droplets Fluorescence Assay Kit (Cat#22735) and Nuclear Green™ LCS1 (Cat#17540).

Citations

View all 4 citations: Citation Explorer

Cell-based no-wash fluorescence assays for compound screens using a fluorescence cytometry plate reader
Authors: Gorshkov, Kirill and Pradhan, Manisha and Xu, Miao and Yang, Shu and Lee, Emily M and Chen, Catherine Z and Shen, Min and Zheng, Wei
Journal: Journal of Pharmacology and Experimental Therapeutics (2020): 500--511

Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry
Authors: Yang, Shu and Xu, Miao and Lee, Emily M and Gorshkov, Kirill and Shiryaev, Sergey A and He, Shihua and Sun, Wei and Cheng, Yu-Shan and Hu, Xin and Tharappel, Anil Mathew and others, undefined
Journal: Cell discovery (2018): 31

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References

View all 65 references: Citation Explorer

In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751

Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534

Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706

Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555

Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93

Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759

Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84

DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154