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Nuclear Red™ LCS2 *5 mM DMSO Solution*

Fluorescence images of HeLa cells stained with MitoLite™ Green FM (Cat. 22695) using fluorescence microscope with a FITC filter set (Green). Live cells were co-stained with nuclei stain Nuclear Red™ LCS2 Cat. 17545 (Red)
Fluorescence images of HeLa cells stained with MitoLite™ Green FM (Cat. 22695) using fluorescence microscope with a FITC filter set (Green). Live cells were co-stained with nuclei stain Nuclear Red™ LCS2 Cat. 17545 (Red)
Ordering information
Price ()
Catalog Number17545
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight719.74
SolventDMSO
Spectral properties
Excitation (nm)651
Emission (nm)681
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Molecular weight
719.74
Excitation (nm)
651
Emission (nm)
681
Our Nuclear Red™ LCS2 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Red™ LCS2 has its red fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with the Cy5 filter sets.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Red™ LCS2 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM138.939 µL694.695 µL1.389 mL6.947 mL13.894 mL
5 mM27.788 µL138.939 µL277.878 µL1.389 mL2.779 mL
10 mM13.894 µL69.47 µL138.939 µL694.695 µL1.389 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)651
Emission (nm)681

Product family


NameExcitation (nm)Emission (nm)
Nuclear Red™ LCS1 *5 mM DMSO Solution*622645
Nuclear Red™ DCS1 *5 mM DMSO Solution*631651

Citations


View all 3 citations: Citation Explorer
Bezafibrate Exerts Neuroprotective Effects in a Rat Model of Sporadic Alzheimer’s Disease
Authors: Lin, Li-Fan and Jhao, Yun-Ting and Chiu, Chuang-Hsin and Sun, Lu-Han and Chou, Ta-Kai and Shiue, Chyng-Yann and Cheng, Cheng-Yi and Ma, Kuo-Hsing
Journal: Pharmaceuticals (2022): 109
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References


View all 65 references: Citation Explorer
In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84
DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154