Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 350 Conjugate

Phalloidin-iFluor® 350 Conjugate is a blue-fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of approximately 345/450 nm.
Instrument Compatibility: Compatible with UV excitation and DAPI fluorescence filter sets.
Applications: Suitable for cytoskeletal imaging, actin filament visualization, and multicolor fluorescence microscopy.

<p>Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Phalloidin-iFluor® 350 Conjugate (Cat#23110, Blue) and nuclei stain Nuclear Red™ DCS1 (Cat#17552, Red), respectively.  </p>

Protocol

SDS

Catalog	Size	Price	Quantity
23110	300 Tests	Price

Overview

This blue fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 350-labeled phalloidin) selectively binds to F-actins. Phalloidin derivatives are convenient probes used at nanomolar concentrations for labeling, identifying, and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin and filamin into stable "islands" of aggregated actin polymers. Yet, it does not interfere with stress fibers, i.e., thick bundles of microfilaments. The property of phalloidin is a valuable tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have been enormously helpful in localizing actin filaments in living or fixed cells and visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in studying actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Citations

View all 34 citations: Citation Explorer

Dysregulated ITGA3/FAK/YAP axis mediates impaired alveolar type II epithelial cells function in COPD

Authors:

Liu, Li and Zhong, Suye and Zhou, Tengfei and Wu, Yixing and Hu, Weiping and Wang, Xiao and Ren, Yue and Wang, Kankai and Lin, Xinhua and Zhang, Jing

Journal:

Journal of Advanced Research (2025)

Biosilica 3D Micromorphology of Geodiidae Sponge Spicules Is Patterned by F-Actin

Authors:

Voronkina, Alona and C{\'a}rdenas, Paco and Adam, J{\"o}rg and Meissner, Heike and Nowacki, Krzysztof and Joseph, Yvonne and Tabachnick, Konstantin R and Ehrlich, Hermann

Journal:

Microscopy research and technique (2025)

Atomic force microscopy--based assessment of multimechanical cellular properties for classification of graded bladder cancer cells and cancer early diagnosis using machine learning analysis

Authors:

Zhu, Xinyao and Qin, Rui and Qu, Kaige and Wang, Zuobin and Zhao, Xuexia and Xu, Wei

Journal:

Acta Biomaterialia (2023): 358--373

Synthetic Collagen-like Polymer That Undergoes a Sol--Gel Transition Triggered by O--N Acyl Migration at Physiological pH

Authors:

Ichise, Shinichiro F and Koide, Takaki

Journal:

International Journal of Molecular Sciences (2022): 1584

Interfacing Perforated Eardrums with Graphene-Based Membranes for Broadband Hearing Recovery

Authors:

Li, Chunyan and Xiong, Zhiyuan and Zhou, Lei and Huang, Weiluo and He, Yushi and Li, Linpeng and Shi, Haibo and Lu, Jiayu and Wang, Jian and Li, Dan and others,

Journal:

Advanced Healthcare Materials (2022): 2201471

References

View all 127 references: Citation Explorer

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B., undefined

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes

Authors:

Luo H, Hallen-Adams HE, Walton JD.

Journal:

J Biol Chem (2009): 18070

Physical properties

Molecular weight	~1100
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026