Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 514 Conjugate

Phalloidin-iFluor® 514 Conjugate is a yellow-fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of 511/527 nm.
Enhanced Photostability: Compatible with TRITC fluorescence microscope filter sets.
Applications: Suitable for actin cytoskeleton staining and multicolor fluorescence microscopy.

Fluorescence image of HeLa cells stained with Phalloidin-iFluor® 514 Conjugate. Fixed HeLa cells were stained with Phalloidin-iFluor® 514 Conjugate (Cat. #23116) for F-actin detection and counterstained with Hoechst 33342 (Cat. #17530) for nuclear staining. Images were acquired using FITC and DAPI filter sets and overlaid to show F-actin in green and nuclei in blue.

Protocol

SDS

Catalog	Size	Price	Quantity
23116	300 Tests	Price

Overview

This yellow fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 514-labeled phalloidin) selectively binds to F-actins. Phalloidin derivatives are convenient probes used at nanomolar concentrations for labeling, identifying, and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin and filamin into stable "islands" of aggregated actin polymers. Yet, it does not interfere with stress fibers, i.e., thick bundles of microfilaments. The property of phalloidin is a valuable tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have been enormously helpful in localizing actin filaments in living or fixed cells and visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in studying actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

References

View all 127 references: Citation Explorer

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B., undefined

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity

Authors:

Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.

Journal:

Toxicol Appl Pharmacol (2009): 21

Physical properties

Molecular weight	~1800
Solvent	DMSO

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026