Phenol Red, sodium salt *CAS 34487-61-6*

Phenol Red, sodium salt

CAS 34487-61-6

Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator that is frequently used in cell biology laboratories. Its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Above pH 8.1, phenol red turns a bright pink (fuschia) color. When cells are grown in tissue culture, the medium in which they grow is held close to the physiological pH. A small amount of phenol red added to the growth media will have a pink-red color under normal conditions. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2460	50 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Phenol Red, sodium salt *CAS 34487-61-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	265.703 µL	1.329 mL	2.657 mL	13.285 mL	26.57 mL
5 mM	53.141 µL	265.703 µL	531.406 µL	2.657 mL	5.314 mL
10 mM	26.57 µL	132.852 µL	265.703 µL	1.329 mL	2.657 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	376.36 g/mol	=	mL	x	mM	=	mmol

References

View all 106 references: Citation Explorer

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract

Authors:

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.

Journal:

J Cataract Refract Surg (2006): 1483

A prospective study on trypan blue capsule staining under air vs under viscoelastic

Authors:

Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.

Journal:

Eye (2006): 820

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity

Authors:

Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.

Journal:

Am J Ophthalmol (2006): 754

Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium

Authors:

Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.

Journal:

Graefes Arch Clin Exp Ophthalmol (2005): 917

Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells

Authors:

Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.

Journal:

Invest Ophthalmol Vis Sci (2005): 304

Physical properties

Molecular weight	376.36
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure
UNSPSC	12352200
CAS	34487-61-6

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