Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Cytocite™ and Qubit™ Fluorometers*

Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit

Optimized for Cytocite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit is a fluorimetric assay kit for quantifying total nucleic acids, including dsDNA, ssDNA, and RNA in samples.

Detection Principle: Helixyte™ Green All fluoresces upon binding nucleic acids.
Sensitivity: Lower detection limit can extend down to 10 pg.
Specificity: Binds dsDNA, ssDNA, and RNA without discrimination.
Workflow: 2-minute room-temperature incubation before reading.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers at Ex/Em 480/530 nm.

Comparison of total nucleic acid dose response using the Qubit™ fluorometer (blue) or CytoCite™ fluorometer (red).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17631	200 Tests	Price

Overview

Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit is designed to rapidly measure the total amounts of nucleic acids, including double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) and RNA in a sample. The kit has all the essential reagents, including Helixyte™ Green ssDNA reagent, dilution buffer, and prediluted DNA standards. Helixyte™ Green All reagent is a sensitive fluorescent nucleic acid probe for measuring the total amounts of nucleic acids in a sample that may contain double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), RNA and long oligonucleotides. Helixyte™ Green All reagent indiscriminately binds to dsDNA, ssDNA and RNA. Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit is optimized for measuring the total amounts of nucleic acids with CytoCite™ or Qubit® fluorometers.

References

View all 13 references: Citation Explorer

Accurate bulk quantitation of droplet digital PCR.

Authors:

Sun, Chen and Liu, Leqian and Vasudevan, Harish N and Chang, Kai-Chun and Abate, Adam R

Journal:

bioRxiv : the preprint server for biology (2021)

Nucleic Acid Quantitation with Log-Linear Response Hybridization Probe Sets.

Authors:

Wu, Lucia R and Fang, John Z and Khodakov, Dmitriy and Zhang, David Yu

Journal:

ACS sensors (2020): 1604-1614

Nucleic Acid Extraction from Human Biological Samples.

Authors:

Mullegama, Sureni V and Alberti, Michael O and Au, Cora and Li, Yan and Toy, Traci and Tomasian, Vanina and Xian, Rena R

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 359-383

MICROFLUIDIC DEVICES FOR LABEL-FREE AND NON-INSTRUMENTED QUANTITATION OF UNAMPLIFIED NUCLEIC ACIDS BY FLOW DISTANCE MEASUREMENT.

Authors:

Chatterjee, Debolina and Mansfield, Danielle S and Woolley, Adam T

Journal:

Analytical methods : advancing methods and applications (2014): 8173-8179

Helicase-dependent amplification of nucleic acids.

Authors:

Cao, Yun and Kim, Hyun-Jin and Li, Ying and Kong, Huimin and Lemieux, Bertrand

Journal:

Current protocols in molecular biology (2013): 15.11.1-15.11.12

Spectral properties

Excitation (nm)	509
Emission (nm)	527

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR tube

CytoCite Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR tube

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Fax
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 14, 2026

Component A: Helixyte™ Green All	1 vial (0.25 mL-200X DMSO)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: Nucleic Acid Standard #1	1 vial (1 mL, 0 ng/µL)
Component D: Nucleic Acid Standard #2	1 vial (1 mL, 10 ng/µL)