Name: Portelite™ Rapid Fluorimetric Endotoxin Detection Kit
Brand: AAT Bioquest
SKU: 60008
Availability: InStock

Portelite™ Rapid Fluorimetric Endotoxin Detection Kit

Portelite™ Rapid Fluorimetric Endotoxin Detection Kit is a fluorimetric assay kit for quantifying endotoxin (LPS) in biological samples.

Detection Principle: Endotoxin activates LAL-mediated hydrolysis of Endotoxin Green™.
Detection Range: Measures approximately 0.002–1 EU/mL endotoxin.
Workflow: Includes a 30-minute LAL incubation before fluorescence detection.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers at Ex 480 nm / Em 510–580 nm.

E.coli Endotoxin dose response was measured in a 0.2 mL, thin-wall PCR tube using CytoCite™ with Green fluorescent channel. As low as 0.002 EU/mL of E.coli Endotoxin can be detected with 10 minutes incubation.

Protocol

COA

Catalog	Size	Price	Quantity
60008	50 Tests	Price
60009	250 Tests	Price

Overview

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria. LPS is a potent stimulator of the vertebrate innate immune system and can cause fever, septic shock and eventually death. It is also recognized as a biomarker for the detection of bacterial pathogen invasion, and responsible for the development of inflammatory response and endotoxic shock in extreme cases. Detection of LPS in biological materials, such as protein, peptide or antibody sample, is a critical task for biomanufacturing and bioprocessing. Portelite™ Rapid Fluorimetric Endotoxin Detection Kit uses Endotoxin Green™, a sensitive fluorogenic substrate. Endotoxin Green™ can be hydrolyzed in the presence of endotoxins and the Limulus Amebocyte Lysate (LAL), an extract of blood cells from a horseshoe crab, to generate strong green fluorescence. The endotoxin activity is proportional to the fluorescence intensity resulted from the hydrolysis of Endotoxin Green™. The kit is optimized for Cytocite™ and Qubit™ fluorometers, and can detect a broad range of endotoxin (from 1 EU/ml to 0.002EU/ml) present in the sample.

Components

Catalog 60008 (50 Tests)

Component A: Endotoxin Green™	1 Vial
Component B: Endotoxin-Free Water	1 Bottle (25 mL)
Component C: Limulus Amebocyte Lysate	1 Vial
Component D: E.coli Endotoxin Standard	1 Vial (100 EU/mL, 100 µL)
Component E: DMSO	1 Vial (100 µL)

Catalog 60009 (250 Tests)

Component A: Endotoxin Green™	1 Vial
Component B: Endotoxin-Free Water	2 Bottles (125 mL/bottle)
Component C: Limulus Amebocyte Lysate	1 Bottle
Component D: E.coli Endotoxin Standard	2 Vials (100 EU/mL, 100 µL)
Component E: DMSO	1 Vial (600 µL)

Citations

View all 33 citations: Citation Explorer

A Validation Study of the Limulus Amebocyte Lysate Test as an End-Product Endotoxin Test for Polyvalent Horse Snake Antivenom

Authors:

Sheraba, N. S., Diab, M. R., Yassin, A. S., Amin, M. A., Zedan, H. H.

Journal:

PDA J Pharm Sci Technol (2019): 562-571

Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

Authors:

Uchida, T., Kaku, Y., Hayasaka, H., Kofuji, M., Momose, N., Miyazawa, H., Ueda, Y., Ito, K., Ookawara, S., Morishita, Y.

Journal:

Med Devices (Auckl) (2019): 429-433

Evaluation of a portable test system for assessing endotoxin activity in raw milk

Authors:

Suzuki, Y., Suzuki, K., Shimamori, T., Tsuchiya, M., Niehaus, A., Lakritz, J.

Journal:

J Vet Med Sci (2016): 49-53

Detection of Endotoxin Contamination of Graphene Based Materials Using the TNF-alpha Expression Test and Guidelines for Endotoxin-Free Graphene Oxide Production

Authors:

Mukherjee, S. P., Lozano, N., Kucki, M., Del Rio-Castillo, A. E., Newman, L., Vazquez, E., Kostarelos, K., Wick, P., Fadeel, B.

Journal:

PLoS One (2016): e0166816

COMPARISON OF QuantiFERON(R) TB GOLD TEST RESULTS BEFORE AND AFTER ENDOTOXIN CONTAMINATION

Authors:

Seto, J., Suzuki, Y., Ahiko, T.

Journal:

Kekkaku (2016): 49-52

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

CytoCite Fluorometer
Excitation	480 nm
Emission	510-580 nm
Instrument specification(s)	0.2 mL, thin-wall PCR tube

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Page updated on October 14, 2026