QXY7 acid [equivalent to QSY-7 NHS ester]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 771.81 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 561 |
| Correction Factor (280 nm) | 0.22 |
| Extinction coefficient (cm -1 M -1) | 900001 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Dyes by Functional Group
Molecular weight 771.81 | Absorbance (nm) 561 | Correction Factor (280 nm) 0.22 | Extinction coefficient (cm -1 M -1) 900001 |
QXY7 is the same molecule to QSY-7 acid (ThermoFisher). QXY7 has a broad and intense absorption at ~550 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications paring with Cy3, TAMRA, Alexa Fluor 555, iFluor 647 or other spectrally similar fluorescent dyes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of QXY7 acid [equivalent to QSY-7 NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 129.566 µL | 647.828 µL | 1.296 mL | 6.478 mL | 12.957 mL |
| 5 mM | 25.913 µL | 129.566 µL | 259.131 µL | 1.296 mL | 2.591 mL |
| 10 mM | 12.957 µL | 64.783 µL | 129.566 µL | 647.828 µL | 1.296 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 561 |
| Correction Factor (280 nm) | 0.22 |
| Extinction coefficient (cm -1 M -1) | 900001 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) | Correction Factor (260 nm) |
| ICG acid | 789 | 814 | - | - | 0.076 | - |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.151 | 0.073 | 0.07 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 | 0.02 |
| Cy7NS acid | 756 | 779 | 250000 | 0.3 | 0.036 | 0.05 |
| AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | - | 0.153 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* | 336 | 455 | 5900 | - | 0.107 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | - | 0.107 | - |
| DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | - | - | - | - | 0.516 | - |
| BCECF acid *CAS#: 85138-49-4* | 504 | 527 | 90000 | - | - | - |
Show More (11) | ||||||
Images
Figure 1. Chemical structure for QXY7 acid [equivalent to QSY-7 NHS ester].
References
View all 14 references: Citation Explorer
Structural exploration and Förster theory modeling for the interpretation of gas-phase FRET measurements: Chromophore-grafted amyloid-β peptides.
Authors: Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: The Journal of chemical physics (2015): 025101
Authors: Kulesza, Alexander and Daly, Steven and MacAleese, Luke and Antoine, Rodolphe and Dugourd, Philippe
Journal: The Journal of chemical physics (2015): 025101
Action-FRET: probing the molecular conformation of mass-selected gas-phase peptides with Förster resonance energy transfer detected by acceptor-specific fragmentation.
Authors: Daly, Steven and Poussigue, Frédéric and Simon, Anne-Laure and MacAleese, Luke and Bertorelle, Franck and Chirot, Fabien and Antoine, Rodolphe and Dugourd, Philippe
Journal: Analytical chemistry (2014): 8798-804
Authors: Daly, Steven and Poussigue, Frédéric and Simon, Anne-Laure and MacAleese, Luke and Bertorelle, Franck and Chirot, Fabien and Antoine, Rodolphe and Dugourd, Philippe
Journal: Analytical chemistry (2014): 8798-804
Fluorescence-based sensing of glucose using engineered glucose/galactose-binding protein: a comparison of fluorescence resonance energy transfer and environmentally sensitive dye labelling strategies.
Authors: Khan, Faaizah and Gnudi, Luigi and Pickup, John C
Journal: Biochemical and biophysical research communications (2008): 102-6
Authors: Khan, Faaizah and Gnudi, Luigi and Pickup, John C
Journal: Biochemical and biophysical research communications (2008): 102-6
A time-resolved, internally quenched fluorescence assay to characterize inhibition of hepatitis C virus nonstructural protein 3-4A protease at low enzyme concentrations.
Authors: Mao, Shi-Shan and DiMuzio, Jillian and McHale, Carolyn and Burlein, Christine and Olsen, David and Carroll, Steven S
Journal: Analytical biochemistry (2008): 1-8
Authors: Mao, Shi-Shan and DiMuzio, Jillian and McHale, Carolyn and Burlein, Christine and Olsen, David and Carroll, Steven S
Journal: Analytical biochemistry (2008): 1-8
Longer wavelength fluorescence resonance energy transfer depsipeptide substrates for hepatitis C virus NS3 protease.
Authors: Konstantinidis, Alex K and Richardson, Paul L and Kurtz, Kevin A and Tripathi, Rakesh and Chen, Chih-Ming and Huang, Peggy and Randolph, John and Towne, Danli and Donnelly, Jennifer and Warrior, Usha and Middleton, Tim and Kati, Warren M
Journal: Analytical biochemistry (2007): 156-67
Authors: Konstantinidis, Alex K and Richardson, Paul L and Kurtz, Kevin A and Tripathi, Rakesh and Chen, Chih-Ming and Huang, Peggy and Randolph, John and Towne, Danli and Donnelly, Jennifer and Warrior, Usha and Middleton, Tim and Kati, Warren M
Journal: Analytical biochemistry (2007): 156-67
M-DNA: A novel metal ion complex of DNA studied by fluorescence techniques.
Authors: Wettig, Shawn D and Wood, David O and Aich, Palok and Lee, Jeremy S
Journal: Journal of inorganic biochemistry (2005): 2093-101
Authors: Wettig, Shawn D and Wood, David O and Aich, Palok and Lee, Jeremy S
Journal: Journal of inorganic biochemistry (2005): 2093-101
Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22
Fluorescence assay of SIRT protein deacetylases using an acetylated peptide substrate and a secondary trypsin reaction.
Authors: Marcotte, Patrick A and Richardson, Paul L and Richardson, Paul R and Guo, Jun and Barrett, Leo W and Xu, Nan and Gunasekera, Angelo and Glaser, Keith B
Journal: Analytical biochemistry (2004): 90-9
Authors: Marcotte, Patrick A and Richardson, Paul L and Richardson, Paul R and Guo, Jun and Barrett, Leo W and Xu, Nan and Gunasekera, Angelo and Glaser, Keith B
Journal: Analytical biochemistry (2004): 90-9
Resonance energy transfer for assessing the molecular integrity of proteins for local delivery.
Authors: Wu, David and Edelman, Elazer R
Journal: Biotechnology and bioengineering (2004): 406-12
Authors: Wu, David and Edelman, Elazer R
Journal: Biotechnology and bioengineering (2004): 406-12
Evaluation of the phosphorescent palladium(II)-coproporphyrin labels in separation-free hybridization assays.
Authors: Burke, Martina and O'Sullivan, Paul J and Soini, Aleksi E and Berney, Helen and Papkovsky, Dmitri B
Journal: Analytical biochemistry (2003): 273-80
Authors: Burke, Martina and O'Sullivan, Paul J and Soini, Aleksi E and Berney, Helen and Papkovsky, Dmitri B
Journal: Analytical biochemistry (2003): 273-80
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)