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ReadiLink™ Rapid iFluor® 405 Antibody Labeling Kit
Microscale Optimized for Labeling 50 µg Antibody Per Reaction
ReadiLink™ Rapid iFluor® 405 Antibody Labeling Kit provides a convenient format for labeling antibodies with violet-excitable iFluor® 405 dye.
  • Microscale optimized: Designed for labeling 50 µg antibody per reaction
  • Two‑step protocol: Labeling consists of simple mixing steps without column purification
  • FeatureiFluor® 405 fluorescence: iFluor® 405 has excitation/emission maxima at 403/427 nm
  • Complete reagent set: Includes buffers and pre‑aliquoted dye for two reactions
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with CD4 (RPA-T4) antibody labeled using ReadiLink™ Rapid iFluor® 405 Antibody Labeling Kit (Cat. #1228). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V1-A channel, demonstrating clear detection of CD4⁺ lymphocytes.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with CD4 (RPA-T4) antibody labeled using ReadiLink™ Rapid iFluor® 405 Antibody Labeling Kit (Cat. #1228). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V1-A channel, demonstrating clear detection of CD4⁺ lymphocytes.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
12282 Labelings
Price
 
Physical properties
SolventDMSO
Spectral properties
Correction factor (260 nm)0.48
Correction factor (280 nm)0.77
Extinction coefficient (cm -1 M -1)
37000
1
Excitation (nm)403
Emission (nm)427
Quantum yield
0.91
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on April 5, 2026