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ReadiLink™ Rapid mFluor™ Red 780 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Red 780 dyes have fluorescence excitation and emission maxima of ~629 nm and ~780 nm respectively. Like Alexa Fluor 750-APC tandem, it has strong absorption at 633 nm, making its conjugates readily excitable by 633 nm red laser. These spectral characteristics make mFluor™ Red 780 an excellent alternative to Alexa Fluor 700-APC tandem. mFluor™ Red 780 is a small organic molecule that is much easier to use than the Alexa Fluor 750-APC tandem. mFluor™ Red 780 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components to perform two separate labeling reactions with no column purification needed. Each of the two vials of mFluor™ Red 780 provided in the kit is optimized for labeling ~50 µg antibody. mFluor™ Red 780 antibody labeling kit provides a convenient method to label small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the mFluor™ Red 780 dye.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Flow cytometric analysis of CD45 expression in differentiated HL-60 cells quantified using an anti-CD45 antibody labeled using the ReadiLink&trade; Rapid mFluor&trade; Red 780 Antibody Labeling Kit (Cat No. 1131).<strong>&nbsp;</strong>HL-60 cells were treated with 1.25% DMSO for 4 days to differentiate. Live cells were then incubated with 1 &micro;g/mL anti-CD45-mFluor&trade; 780 or IgG-mFluor&trade; 780 control and analyzed by NovoCyte.
Flow cytometric analysis of CD45 expression in differentiated HL-60 cells quantified using an anti-CD45 antibody labeled using the ReadiLink&trade; Rapid mFluor&trade; Red 780 Antibody Labeling Kit (Cat No. 1131).<strong>&nbsp;</strong>HL-60 cells were treated with 1.25% DMSO for 4 days to differentiate. Live cells were then incubated with 1 &micro;g/mL anti-CD45-mFluor&trade; 780 or IgG-mFluor&trade; 780 control and analyzed by NovoCyte.
CatalogSize
Price
Quantity
11312 Labelings
Price
 
Spectral properties

Absorbance (nm)630
Correction factor (260 nm)0.101
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)629
Emission (nm)767
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 30, 2025