Name: ReadiUse™ RBC Lysis/Fixation Solution
Brand: AAT Bioquest
SKU: 37012
Availability: InStock

ReadiUse™ RBC Lysis/Fixation Solution

ReadiUse™ RBC Lysis/Fixation Solution is a one-step reagent that simultaneously lyses red blood cells and fixes leukocytes while preserving phosphorylation states, ideal for intracellular flow cytometry.

One-Step RBC Lysis & Leukocyte Fixation: Simplifies intracellular flow cytometry by combining lysis and fixation in a single step
Preserves Phosphorylation States: Maintains in vivo phosphorylation for accurate intracellular signaling analysis
Flow Cytometry Optimized: Ensures cell integrity and light-scattering properties for reliable results
Validated for Human & Mouse Blood: Effective for both species and comparable to traditional PBMC isolation methods
Flexible Protocols: Compatible with phospho-specific antibody staining and lyse/wash or no-wash protocols

General packaging for Cat. No. 37012 and 37013. Your actual packaging may differ.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
37012	50 mL	Price
37013	100 mL	Price

Overview

The ReadiUse™ RBC Lysis/Fixation Solution is specifically formulated for efficient red blood cell lysis and concurrent leukocyte fixation in whole blood, making it ideal for intracellular flow cytometry applications. Following cell stimulation, such as with kinase activators like phorbol esters, the solution provides a one-step process that lyses RBCs and fixes leukocytes, effectively inhibiting kinases, phosphatases, and proteases. This preserves the phosphorylation state as it exists in vivo, enabling precise analysis of intracellular signaling events. The ReadiUse™ RBC Lysis/Fixation Solution is optimized to maintain the light-scattering properties of cells, ensuring that cell integrity is preserved during flow cytometric analysis. It is validated for use with both human and mouse whole blood, providing RBC lysis that is as effective as density gradient centrifugation in isolating peripheral blood mononuclear cells (PBMCs) and performing lymphocyte subset analyses. Moreover, the reagent is compatible with phospho-specific antibody staining in conjunction with surface markers (e.g., CD markers). The ReadiUse™ solution is suitable for both lyse/wash and lyse/no-wash immunofluorescent staining protocols, offering flexibility depending on the experimental requirements.

References

View all 50 references: Citation Explorer

"Candidatus Siderophilus nitratireducens": a putative nap-dependent nitrate-reducing iron oxidizer within the new order Siderophiliales.

Authors:

Corbera-Rubio, Francesc and Stouten, Gerben R and Bruins, Jantinus and Dost, Simon F and Merkel, Alexander Y and Müller, Simon and van Loosdrecht, Mark C M and van Halem, Doris and Laureni, Michele

Journal:

ISME communications (2024): ycae008

Risk factors for allogeneic red blood cell transfusion in adult spinal deformity surgery.

Authors:

Iijima, Yasushi and Kotani, Toshiaki and Sakuma, Tsuyoshi and Akazawa, Tsutomu and Kishida, Shunji and Ueno, Keisuke and Ise, Shohei and Ogata, Yosuke and Mizutani, Masaya and Shiga, Yasuhiro and Minami, Shohei and Ohtori, Seiji

Journal:

Asian spine journal (2024): 579-586

Latent infections in conversion total hip arthroplasty following internal fixation of femoral neck fractures: a systematic review and meta-analysis of diagnostic methods.

Authors:

Poursalehian, Mohammad and Lotfi, Mohadeseh and Mortazavi, Seyed Mohammad Javad

Journal:

Archives of orthopaedic and trauma surgery (2024)

The effects of two fixation methods on blood loss in patients with trochanteric fracture: dynamic hip screw vs. proximal femoral nail anti-rotation.

Authors:

Sivro, Mirza and Lazović, Faruk and Omerović, Ðemil and Papović, Adnan

Journal:

Medicinski glasnik : official publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina (2023): 249-254

Assistive diagnostic indicators for infections related to lumbar posterior interbody fusion internal fixation: platelet count and mean platelet volume.

Authors:

Zhang, Yadong and Li, Houkun and Wang, Wentao and Shan, Lequn and Hao, Dingjun

Journal:

Journal of orthopaedic surgery and research (2023): 883

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on January 4, 2026