Rhod-2 AM | AAT Bioquest

Rhod-2, AM

CAS#: 145037-81-6

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence. Rhod-2 AM is cell-permeable version of Rhod-2.

<strong>The intracellular distribution of free Ca<sup>2+</sup> was analyzed by laser scanning confocal microscopy.</strong><br />Intracellular free Ca<sup>2+</sup> was detected by red fluorescent probe dihydrorhod-2 AM (Rhod-2 AM). The nuclei were stained with DAPI (blue). The pEZ-LV203 vector harboring the eGFP reporter gene produced green fluorescent protein. Source: <strong>Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca<sup>2+</sup> dyshomeostasis in hepatocarcinoma PLC cells </strong>by Shi M et al., <em>PLOS,</em> <em> </em>Nov. 2017.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21060	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-2, AM *CAS#: 145037-81-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.971 µL	444.856 µL	889.711 µL	4.449 mL	8.897 mL
5 mM	17.794 µL	88.971 µL	177.942 µL	889.711 µL	1.779 mL
10 mM	8.897 µL	44.486 µL	88.971 µL	444.856 µL	889.711 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1123.96 g/mol	=	mL	x	mM	=	mmol

Citations

View all 34 citations: Citation Explorer

Activation of store-operated calcium entry and mitochondrial respiration by enterovirus 71 is essential for efficient virus replication

Authors:

Hsu, Bang-Yan and Tsai, Ya-Hui and Weng, Ta-Chou and Kung, Szu-Hao

Journal:

mBio (2025): e03717--24

Evidence for the cardiodepressive effects of the plasticizer di-2-ethylhexylphthalate (DEHP)

Authors:

Swift, Luther M and Roberts, Anysja and Pressman, Jenna and Guerrelli, Devon and Allen, Samuel and Haq, Kazi T and Reisz, Julie A and D’Alessandro, Angelo and Posnack, Nikki Gillum

Journal:

bioRxiv (2023): 2023--05

Alterations in Mitochondria-Associated Endoplasmic Reticulum Membranes Under Oxidative Stress in R28 Cells

Authors:

Yang, Yuting and Wu, Jihong and Lu, Wei and Dai, Yiqin and Zhang, Youjia and Sun, Xinghuai

Dynamic changes in $\beta$-cell [Ca2+] regulate NFAT activation, gene transcription, and islet gap junction communication

Authors:

Miranda, Jose G and Schleicher, Wolfgang E and Wells, Kristen L and Ramirez, David G and Landgrave, Samantha P and Benninger, Richard KP

Journal:

Molecular metabolism (2022): 101430

Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome

Authors:

Yoon, Jin-Young and Daneshgar, Nastaran and Chu, Yi and Chen, Biyi and Hefti, Marco and Irani, Kaikobad and Song, Long-Sheng and Brenner, Charles and Abel, E Dale and London, Barry and others,

Journal:

bioRxiv (2022)

References

View all 8 references: Citation Explorer

Protein kinase C and myocardial calcium handling during ischemia and reperfusion: lessons learned using Rhod-2 spectrofluorometry

Authors:

Stamm C, del Nido PJ.

Journal:

Thorac Cardiovasc Surg (2004): 127

Cytosolic calcium in the ischemic rabbit heart: assessment by pH- and temperature-adjusted rhod-2 spectrofluorometry

Authors:

Stamm C, Friehs I, Choi YH, Zurakowski D, McGowan FX, del Nido PJ.

Journal:

Cardiovasc Res (2003): 695

Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory

Authors:

Du C, MacGowan GA, Farkas DL, Koretsky AP.

Journal:

Biophys J (2001): 549

Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching

Authors:

Du C, MacGowan GA, Farkas DL, Koretsky AP.

Journal:

Cell Calcium (2001): 217

Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions

Authors:

Lannergren J, Westerblad H, Bruton JD.

Journal:

J Muscle Res Cell Motil (2001): 265

Physical properties

Dissociation constant (K_d, nM)	570
Molecular weight	1123.96
Solvent	DMSO

Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	145037-81-6

Instrument settings

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	540
Emission	590
Cutoff	570
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on September 20, 2024