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Streptavidin-Xtra™ IF555
Streptavidin conjugates are widely used in combing with biotin conjugates for detecting a variety of biological targets such as proteins, nucleic acids and other molecules. They are used as an ideal choice for many biological detections such as immunofluorescence microscopy, flow cytometry, western blot and other biological applications since streptavidin has a strong affinity binding biotin which is not affected over a broad range of pH and temperature. AAT Bioquest® offers a variety of streptavidin conjugates labeled with the classic fluorescent dyes (for example: FITC, TRITC, Texas Red®, Cy3®, Cy5® and Cy7®) and also our superior water soluble, photostable iFluor® and mFluor™ dyes. However, the conventional biotin-avidin detection systems are still limited by the limited signal intensity of the existing fluorescent conjugates. The Streptavidin Xtra™ iFluor® conjugates are a new family of super bright streptavidin conjugates with nearly identical excitation and emission properties to Alexa Fluor fluorophores with 3~5 folds signal improvement. It is a set of powerful tools to detect low abundance targets in cell imaging or flow cytometry. iFluor® 555 is one of the most common orange/red fluorescence colors for the Cy3 channel imaging.
Images of Hela cells stained with Streptavidin-Xtra&trade; iFluor® conjugates and Streptavidin Alexa Fluor &trade; conjugate.<br />Hela cells were fixed with 4% paraformaldehyde for 30 minutes, permeabilized with 0.02% Triton&trade; X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. Fixed Hela cells were then stained with 1 &micro;g/mL alpha Tubulin Mouse Monoclonal Antibody for 1 hour at room temperature, followed by GxM IgG-biotin (Cat# 16729) stain and then visualized with Streptavidin-Xtra&trade; iFluor 555 and Streptavidin-Alexa Fluor&trade; 555 . &nbsp;Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
Images of Hela cells stained with Streptavidin-Xtra&trade; iFluor® conjugates and Streptavidin Alexa Fluor &trade; conjugate.<br />Hela cells were fixed with 4% paraformaldehyde for 30 minutes, permeabilized with 0.02% Triton&trade; X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. Fixed Hela cells were then stained with 1 &micro;g/mL alpha Tubulin Mouse Monoclonal Antibody for 1 hour at room temperature, followed by GxM IgG-biotin (Cat# 16729) stain and then visualized with Streptavidin-Xtra&trade; iFluor 555 and Streptavidin-Alexa Fluor&trade; 555 . &nbsp;Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
protocol
Protocol
COA
CatalogSize
Price
Quantity
46002100 ug
Price
460031 mg
Price
 
Spectral properties
Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling
Intended useResearch Use Only (RUO)
Instrument settings
Flow cytometer
Excitation532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet (Catalog 46002)
Safety Data Sheet (Catalog 46003)
Certificate of Analysis
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Page updated on October 30, 2025