Streptavidin-Xtra™ IF647
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (656 nm) | 0.0793 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 656 |
Emission (nm) | 670 |
Quantum yield | 0.251 |
Intended use | Research Use Only (RUO) |
Overview | SDSProtocol |
Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.03 | Correction Factor (656 nm) 0.0793 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 656 | Emission (nm) 670 | Quantum yield 0.251 |
Platform
Flow cytometer
Excitation | 633 nm laser |
Emission | 660/20 nm filter |
Instrument specification(s) | APC channel |
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 µL (For Cat# 46006) or 1 mL (For Cat# 46007) of ddH2O to the vial to make a 1 mg/mL stock solution.
Note: This constituted stock solution will be in PBS with 0.2% BSA.
PREPARATION OF WORKING SOLUTION
For IF, the suggested staining concentration is 1-5 ug/ml. For FACS, the suggested concentration is at 0.1-0.5 ug / 100 uL / million cells in the staining buffer.
Note: PBS + 0.1% BSA can be used as a staining buffer.
Note: For the best performance of each application, the optimal concentration of this reagent needs to be carefully determined.
Note: The suggested working dilution is provided as a guide only. It is recommended that the users titrate the product in their tests using proper positive and negative controls.
SAMPLE EXPERIMENTAL PROTOCOL
- Block and treat the samples with antibodies of interest as per the manufacturer's recommendations.
Add biotin-conjugated secondary antibody working solution in the samples at appropriate concentration and duration.]
Note: Please verify the compatibility and type of your biotin-conjugated antibody with the primary antibody used in the experiment. For example, If the primary antibody is a mouse antibody, then a goat Anti-Mouse antibody bound with biotin can be used for the assay.
- Incubate the cells with Streptavidin-Xtra™ working solution at room temperature for 30 minutes to 1 hour. Note: Optimal time for incubation needs to be determined carefully.
- Remove the working solution and resuspend the cells in your choice of buffer.
- Take the image using the fluorescence microscope or record the intensity using flow cytometer.
Spectrum
Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (656 nm) | 0.0793 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 656 |
Emission (nm) | 670 |
Quantum yield | 0.251 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Streptavidin-Xtra™ IF488 | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
Streptavidin-Xtra™ IF555 | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
Streptavidin-Xtra™ IF594 | 568 | 587 | 1000001 | 0.571 | 0.34 | 0.15 |
Images
Hela cells were fixed with 4% paraformaldehyde for 30 minutes, permeabilized with 0.02% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. Fixed Hela cells were then stained with 1 µg/mL alpha Tubulin Mouse Monoclonal Antibody for 1 hour at room temperature, followed by GxM IgG-biotin (Cat# 16729) stain and then visualized with Streptavidin-Xtra™ iFluor 647 and Streptavidin-Alexa Fluor™ 647. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
Citations
Authors: Jia, Liqiu and Weng, Shufeng and Wu, Jing and Tian, Xiangxiang and Zhang, Yifan and Wang, Xuyang and Wang, Jing and Yan, Dongmei and Wang, Wanhai and Fang, Fang and others,
Journal: Gut microbes (2022): 2117503
Authors: Wei, Y. P., Liu, X. P., Mao, C. J., Niu, H. L., Song, J. M., Jin, B. K.
Journal: Biosens Bioelectron (2018): 99-103
Authors: Yang, Z., Lan, Q., Li, J., Wu, J., Tang, Y., Hu, X.
Journal: Biosens Bioelectron (2017): 312-318
Authors: Hao, K., He, Y., Lu, H., Pu, S., Zhang, Y., Dong, H., Zhang, X.
Journal: Anal Chim Acta (2017): 114-120
Authors: Mishina, M., Minamihata, K., Moriyama, K., Nagamune, T.
Journal: Biomacromolecules (2017): 311
Authors: Guo, Q., Han, J. J., Shan, S., Liu, D. F., Wu, S. S., Xiong, Y. H., Lai, W. H.
Journal: Biosens Bioelectron (2016): 990-995
Authors: Chen, J., Liu, Y., Ji, X., He, Z.
Journal: Biosens Bioelectron (2016): 221-8
Authors: Lakshmipriya, T., Gopinath, S. C., Tang, T. H.
Journal: PLoS One (2016): e0151153
Authors: Mishina, M., Minamihata, K., Moriyama, K., Nagamune, T.
Journal: Biomacromolecules (2016): 1978-84
Authors: Mortberg, A., Meinke, S., Berg, P., Killie, M. K., Kjeldsen-Kragh, J., Jaras, K., Refsum, E., Hoglund, P., Wikman, A.
Journal: J Immunol Methods (2016): 9-15