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Tide Fluor™ 1 azide [TF1 azide]

TF1 is designed to be a superior fluorophore alternative to EDANS. TF1 has (a). much stronger absorption; (b). much stronger fluorescence intensity; and (3). much less environment-sensitive fluorescence. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to EDANS. TF1-labeled peptides and nucleotides exhibit much stronger fluorescence and higher sensitivity than the ones labeled with EDANS. In pairing with our Tide Quencher™ 1 (TQ1), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF1 product is reactive to alkynes, and useful for click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 1 azide [TF1 azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM331.895 µL1.659 mL3.319 mL16.595 mL33.19 mL
5 mM66.379 µL331.895 µL663.79 µL3.319 mL6.638 mL
10 mM33.19 µL165.948 µL331.895 µL1.659 mL3.319 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Page updated on October 12, 2024

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Catalog Number2236
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Physical properties

Molecular weight

301.30

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.187

Extinction coefficient (cm -1 M -1)

20000

Excitation (nm)

341

Emission (nm)

448

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).