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Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS*

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Ordering information
Price ()
Catalog Number2242
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight355.34
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.187
Extinction coefficient (cm -1 M -1)20000
Excitation (nm)341
Emission (nm)448
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Tide Fluor™ 3 azide [TF3 azide]
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Tide Fluor™ 5WS azide [TF5WS azide]
Tide Fluor™ 5WS alkyne [TF5WS alkyne]
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Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red*
Tide Fluor™ 4 amine [TF4 amine] *Superior replacement for ROX and Texas Red*
Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red*
Tide Fluor™ 6WS acid [TF6WS acid] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]*Superior replacement for Cy5.5*
Tide Fluor™ 4 azide [TF4 azide]
Tide Fluor™ 4 alkyne [TF4 alkyne]
Tide Fluor™ 6WS azide [TF6WS azide]
Tide Fluor™ 6WS alkyne [TF6WS alkyne]
Tide Fluor™ 7WS azide [TF7WS azide]
Tide Fluor™ 7WS alkyne [TF7WS alkyne]
Tide Fluor™ 8WS azide [TF8WS azide] *Near Infrared Emission*
Tide Fluor™ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*
Tide Fluor™ 7WS acid [TF7WS acid] *Superior replacement for Cy7*
Tide Fluor™ 7WS amine [TF7WS amine] *Superior replacement for Cy7*
Tide Fluor™ 7WS, succinimidyl ester [TF7WS SE]*Superior replacement for Cy7*
Tide Fluor™ 8WS acid [TF8WS acid] *Near Infrared Emission*
Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission*
Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*
Tide Fluor™ 3WS acid [TF3WS acid] *Superior replacement for Cy3*
Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3*
Tide Fluor™ 3WS amine [TF3WS amine] *Superior replacement for Cy3*
Tide Fluor™ 2WS acid [TF2WS acid] *Superior replacement for FITC*
Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC*
Tide Fluor™ 2WS Amine [TF2WS amine] *Superior replacement for FITC*
Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*
Show More (43)

OverviewpdfSDSpdfProtocol


Molecular weight
355.34
Correction Factor (280 nm)
0.187
Extinction coefficient (cm -1 M -1)
20000
Excitation (nm)
341
Emission (nm)
448
TF1 is designed to be a superior fluorophore alternative to EDANS. TF1 has (a). much stronger absorption; (b). much stronger fluorescence intensity; and (3). much less environment-sensitive fluorescence. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to EDANS. TF1-labeled peptides and nucleotides exhibit much stronger fluorescence and higher sensitivity than the ones labeled with EDANS. In pairing with our Tide Quencher™ 1 (TQ1), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM281.421 µL1.407 mL2.814 mL14.071 mL28.142 mL
5 mM56.284 µL281.421 µL562.841 µL2.814 mL5.628 mL
10 mM28.142 µL140.71 µL281.421 µL1.407 mL2.814 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.187
Extinction coefficient (cm -1 M -1)20000
Excitation (nm)341
Emission (nm)448

Citations


View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

References


View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519