Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3*
Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.
![With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3%2Ffigure-for-tide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3_0ATQK.jpg&w=640&q=75)
![With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3%2Ffigure-for-tide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3_0ATQK.jpg&w=640&q=75)
![With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ftide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3%2Ffigure-for-tide-fluor-3-acid-tf3-acid-superior-replacement-for-cy3_0ATQK.jpg&w=128&q=25)
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 178.667 µL | 893.336 µL | 1.787 mL | 8.933 mL | 17.867 mL |
5 mM | 35.733 µL | 178.667 µL | 357.334 µL | 1.787 mL | 3.573 mL |
10 mM | 17.867 µL | 89.334 µL | 178.667 µL | 893.336 µL | 1.787 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
Tide Fluor™ 2 acid [TF2 acid] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 3 azide [TF3 azide] | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 3 alkyne [TF3 alkyne] | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 5WS acid [TF5WS acid] *Superior replacement for Cy5* | 649 | 664 | 250000 | 0.027 |
Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red* | 578 | 602 | 90000 | 0.436 |
Tide Fluor™ 6WS acid [TF6WS acid] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
Tide Fluor™ 7WS acid [TF7WS acid] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
Tide Fluor™ 8WS acid [TF8WS acid] *Near Infrared Emission* | 785 | 801 | 250000 | 0.109 |
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Citations
View all 8 citations: Citation Explorer
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
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Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
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