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Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red*

With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weight562.65
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.436
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)578
Emission (nm)602
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red*
Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5*
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Tide Fluor™ 6WS azide [TF6WS azide]
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Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission*
Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*
Tide Fluor™ 3WS maleimide [TF3WS maleimide] *Superior replacement for Cy3*
Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3*
Tide Fluor™ 3WS amine [TF3WS amine] *Superior replacement for Cy3*
Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC*
Tide Fluor™ 2WS maleimide [TF2WS Maleimide] *Superior replacement for FITC*
Tide Fluor™ 2WS Amine [TF2WS amine] *Superior replacement for FITC*
Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*
Show More (35)

OverviewpdfSDSpdfProtocol


Molecular weight
562.65
Correction Factor (280 nm)
0.436
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
578
Emission (nm)
602
TF4 is designed to be a superior fluorophore alternative to ROX and Texas Red®. TF4 has (a). stronger fluorescence intensity; (b). higher conjugation yield; and (c). longer shelf life. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to ROX and Texas Red®. In pairing with our Tide Quencher™ 4 (TQ4), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM177.73 µL888.652 µL1.777 mL8.887 mL17.773 mL
5 mM35.546 µL177.73 µL355.461 µL1.777 mL3.555 mL
10 mM17.773 µL88.865 µL177.73 µL888.652 µL1.777 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.436
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)578
Emission (nm)602

Images


Citations


View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on &beta;-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

References


View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519