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Tide Quencher™ 1 azide [TQ1 azide]

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
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Physical properties
Molecular weight358.42
SolventDMSO
Spectral properties
Absorbance (nm)492
Correction Factor (260 nm)0.147
Correction Factor (280 nm)0.194
Extinction coefficient (cm -1 M -1)20000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Tide Quencher™ 6WS maleimide [TQ6WS maleimide]
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Tide Quencher™ 7WS acid [TQ7WS acid]
Tide Quencher™ 7WS amine [TQ7WS amine]
Tide Quencher™ 7WS maleimide [TQ7WS maleimide]
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]
Tide Quencher™ 1 succinimidyl ester [TQ1 SE]
Tide Quencher™ 2 acid [TQ2 acid]
Tide Quencher™ 2 amine [TQ2 amine]
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*
Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]
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Tide Quencher™ 3 acid [TQ3 acid]
Tide Quencher™ 3 amine [TQ3 amine]
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*
Tide Quencher™ 3 maleimide [TQ3 maleimide]
Tide Quencher™ 3WS acid [TQ3WS acid]
Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]
Tide Quencher™ 3 succinimidyl ester [TQ3 SE]
Tide Quencher™ 3 alkyne [TQ3 alkyne]
Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]
Tide Quencher™ 5.1WS acid [TQ5.1WS acid]
Tide Quencher™ 5.1WS amine [TQ5.1WS amine]
Tide Quencher™ 5.1 CPG [TQ5.1 CPG] *500 Å*
Tide Quencher™ 5.1 CPG [TQ5.1 CPG] *1000 Å*
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide]
Tide Quencher™ 5.1WS succinimidyl ester [TQ5.1WS SE]
Tide Quencher™ 5.1WS azide [TQ5.1WS azide]
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Tide Quencher™ 7.1WS acid [TQ7.1WS acid]
Tide Quencher™ 7.1WS amine [TQ7.1WS amine]
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Tide Quencher™ 7.1 CPG [TQ7.1 CPG] *1000 Å*
Tide Quencher™ 7.1WS maleimide [TQ7.1WS maleimide]
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Tide Quencher™ 7.1WS azide [TQ7.1WS azide]
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Tide Quencher™ 7.2WS acid [TQ7.2WS acid]
Tide Quencher™ 7.2WS amine [TQ7.2WS amine]
Tide Quencher™ 7.2 CPG [TQ7.2 CPG] *500 Å*
Tide Quencher™ 7.2 CPG [TQ7.2 CPG] *1000 Å*
Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide]
Tide Quencher™ 7.2WS succinimidyl ester [TQ7.2WS SE]
Tide Quencher™ 7.2WS azide [TQ7.2WS azide]
Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne]
Tide Quencher™ 8WS acid [TQ8WS acid]
Tide Quencher™ 8WS amine [TQ8WS amine]
Tide Quencher™ 8 CPG [TQ8 CPG] *500 Å*
Tide Quencher™ 8WS maleimide [TQ8WS maleimide]
Tide Quencher™ 8WS succinimidyl ester [TQ8WS SE]
Tide Quencher™ 8WS alkyne [TQ8WS alkyne]
Tide Quencher™ 8 CPG [TQ8 CPG] *1000 Å*
Show More (68)

OverviewpdfSDSpdfProtocol


Molecular weight
358.42
Absorbance (nm)
492
Correction Factor (260 nm)
0.147
Correction Factor (280 nm)
0.194
Extinction coefficient (cm -1 M -1)
20000
TQ1 is designed to be a superior quencher alternative to DABCYL. Compared to DABCYL, TQ1 have (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ1 product is reactive to alkynes, and useful for click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 1 azide [TQ1 azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM279.002 µL1.395 mL2.79 mL13.95 mL27.9 mL
5 mM55.8 µL279.002 µL558.005 µL2.79 mL5.58 mL
10 mM27.9 µL139.501 µL279.002 µL1.395 mL2.79 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)492
Correction Factor (260 nm)0.147
Correction Factor (280 nm)0.194
Extinction coefficient (cm -1 M -1)20000

Images


Citations


View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on &beta;-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

References


View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
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Journal: Biotechniques (2009): 519