Name: Tris NTA azide
Brand: AAT Bioquest
SKU: 12635
Price: 750 USD
Availability: InStock

Tris NTA azide

Tris-NTA Azide combines high-affinity His-tag binding with click chemistry compatibility, enabling site-specific labeling of polyhistidine-tagged proteins.

Trivalent His-Tag Chelator: Three NTA groups provide nM affinity for His-tagged proteins
Multivalent Binding Advantage: Delivers markedly more stable protein complexes compared to transient mono-NTA binding
Bioorthogonal Click Chemistry: Azide group compatible with both CuAAC and strain-promoted cycloaddition (SPAAC) reactions
Selective Protein Detection: Enables His-tag specific labeling in cell lysates and on live cell surfaces for purification and imaging

The chemical structure of Tris NTA azide.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12635	1 mg	Price

Overview

Tris NTA azide is an excellent building block to develop probes for detecting polyHIS tag proteins via the well-known click chemistry. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tris NTA azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.16 µL	420.801 µL	841.602 µL	4.208 mL	8.416 mL
5 mM	16.832 µL	84.16 µL	168.32 µL	841.602 µL	1.683 mL
10 mM	8.416 µL	42.08 µL	84.16 µL	420.801 µL	841.602 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1188.21 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Heterogeneous biocatalysis by magnetic nanoparticle immobilized biomass-degrading enzymes derived from microbial cultures.

Authors:

Gupta, Mani and Bandyopadhyay, Arghya and Sinha, Sushant Kumar and Suksham, and Samanta, Abhisek and Mondal, Surajit and Bhattacharyya, Sayan and Datta, Supratim

Journal:

Journal of materials chemistry. B (2025): 3644-3652

Fabrication of lignin-based sub-micro hybrid particle as a novel support for adenylate cyclase immobilization.

Authors:

Jiang, Dahai and Xu, Ting and Xiao, Honggang and Xu, Wangbo and Zhu, Qingqing and Liu, Dong and Chen, Yong and Ying, Hanjie and Niu, Huanqing and Li, Ming

Journal:

Colloids and surfaces. B, Biointerfaces (2024): 113658

The chromatin remodeler SMARCA5 binds to d-block metal supports: Characterization of affinities by IMAC chromatography and QM analysis.

Authors:

Andrikopoulos, Prokopis C and Čabart, Pavel

Journal:

PloS one (2024): e0309134

Simultaneous Identification of Vitamins B1, B3, B5, and B6 by an Engineered Nanopore.

Authors:

Ouyang, Yusheng and Wang, Kefan and Jia, Wendong and Zhang, Panke and Huang, Shuo

Journal:

Nano letters (2024): 11944-11953

Histidine tag modified magnetic beads for analysis of arsenic binding proteins.

Authors:

Nan, Kai and He, Man and Chen, Beibei and Hu, Bin

Journal:

Analytica chimica acta (2024): 342554

Physical properties

Molecular weight	1188.21
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 15, 2025