Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | ~1000 |
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 609 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Cell Viability Assays
Molecular weight ~1000 | Absorbance (nm) 609 |
Trypan UltraBlue™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan UltraBlue is less toxic to cells, in particular, having minimal effect on cell surface receptors. Our Trypan UltraBlue™ sodium salt is highly purified. Our Trypan UltraBlue™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan UltraBlue™ is highly purified, and can be used up to 0.5 mM with minimal cell cytotoxicity. Our recommended concentration is 0.25 mM.
Images
Figure 1. The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
References
View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132
Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702
Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938
Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208
Application notes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V