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Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
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ShippingStandard overnight for United States, inquire for international
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Physical properties
Molecular weight~1000
Spectral properties
Absorbance (nm)609
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Trypan UltraBlue™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan UltraBlue is less toxic to cells, in particular, having minimal effect on cell surface receptors. Our Trypan UltraBlue™ sodium salt is highly purified. Our Trypan UltraBlue™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan UltraBlue™ is highly purified, and can be used up to 0.5 mM with minimal cell cytotoxicity. Our recommended concentration is 0.25 mM.


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Spectral properties

Absorbance (nm)609



View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304
Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208
Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938