Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

Trypan UltraBlue™, sodium salt

0.1 M aqueous solution

Trypan UltraBlue™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan UltraBlue is less toxic to cells, in particular, having minimal effect on cell surface receptors. Our Trypan UltraBlue™ sodium salt is highly purified. Our Trypan UltraBlue™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan UltraBlue™ is highly purified, and can be used up to 0.5 mM with minimal cell cytotoxicity. Our recommended concentration is 0.25 mM.

<p>The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2455	1 mL	Price

References

View all 106 references: Citation Explorer

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract

Authors:

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.

Journal:

J Cataract Refract Surg (2006): 1483

A prospective study on trypan blue capsule staining under air vs under viscoelastic

Authors:

Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.

Journal:

Eye (2006): 820

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity

Authors:

Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.

Journal:

Am J Ophthalmol (2006): 754

Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium

Authors:

Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.

Journal:

Graefes Arch Clin Exp Ophthalmol (2005): 917

Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells

Authors:

Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.

Journal:

Invest Ophthalmol Vis Sci (2005): 304

Physical properties

Molecular weight	~1000
Solvent	Water

Spectral properties

Absorbance (nm)

609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025