Trypan UltraBlue™, sodium salt 0.1 M aqueous solution

The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	~1000
Solvent	Water

Spectral properties

Absorbance (nm)

609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

~1000

Absorbance (nm)

609

Trypan UltraBlue™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan UltraBlue is less toxic to cells, in particular, having minimal effect on cell surface receptors. Our Trypan UltraBlue™ sodium salt is highly purified. Our Trypan UltraBlue™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan UltraBlue™ is highly purified, and can be used up to 0.5 mM with minimal cell cytotoxicity. Our recommended concentration is 0.25 mM.

Spectrum

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Spectral properties

Absorbance (nm)

609

Images

Figure 1. The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).

References

View all 106 references: Citation Explorer

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483

A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754

Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132

Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702

Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938

Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395

Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152

Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208