AAT Bioquest

Application Notes


Coomassie staining is a widely used technique for visualizing proteins present in SDS-PAGE gels, which is essential for protein detection and analysis in biochemical research. The detection, quantification, and analysis of various proteins allow researchers and scientists to better investigate

Posted July 15, 2024

Antibody labeling (also, bioconjugation) is a fundamental technique in biomedical research and diagnostics, enabling the precise attachment of molecules to antibodies for a wide range of in vitro and in vivo applications. There are several methods for attaching functional ligands to antibodies,

Posted June 21, 2024

Western blot is a technique used to identify specific proteins from a complex mixture of proteins. Prior to Western blot, samples undergo a size-based protein separation step through gel electrophoresis. Western blot includes the transfer of size-based separated proteins onto a solid support and

Posted May 24, 2024

Flow cytometry is a technology used to rapidly analyze cells or particles in a buffered salt-based solution. Flow cytometry uses one, or multiple, lasers to determine the visible light scatter given off by each cell. This light scatter is measured in two specific directions, forward and sideways,

Posted May 2, 2024

Cell lysis, also known as cell disruption, is a common laboratory technique aimed at extracting target nucleic acids or proteins inside the cell. In cell lysis or cellular disruption is a method in which the cell membrane is broken down or destroyed. In this process, subcellular components like DNA,

Posted April 30, 2024