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AAT Bioquest

Application Notes

 


Gel Staining Gel electrophoresis stains are chemicals used to visualize and identify fragmented proteins, DNA, and RNA in a gel. Stains for nucleic acids are typically fluorescent which can intercalate into their double helix structure. Common stains for proteins include Coomassie blue, silver,

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Posted August 28, 2023

The concentration of the gel is crucial for any gel electrophoresis experiment, and the optimal gel concentration, regardless of matrix type, for an experiment depends on the sizes of the starting proteins and desired fragments size.   Agarose Gel For any gel used in electrophoresis, the concentration

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Posted August 28, 2023

Gel electrophoresis is a technique that uses electrical current to separate DNA, RNA or other proteins based on their size, shape, and charge. Gel construction is simple, quick, and relatively inexpensive and finished gels can have the consistency of a firm Jello to a rubberier substance, depending

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Posted August 28, 2023

Gel electrophoresis is the fractionation of a protein, DNA, or RNA based on the size, shape, and/or net charges of the macromolecules. Briefly, charged particles are forced to migrate from one direction to another under the influence of an externally applied electric field. The gel matrix acts as a

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Posted August 22, 2023

The Degree of Labeling (DOL), also referred to as the Degree of Substitution (DOS), plays a crucial role in characterizing and optimizing bioconjugates, particularly those involving fluorophore-labeled proteins. Expressed as a molar ratio representing the label-to-protein proportion, the

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Posted May 16, 2023