logo
AAT Bioquest

Posted on May 30, 2023
How do multicellular organisms reproduce?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on May 30, 2023
What is the significance of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 30, 2023
What are the examples of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 30, 2023
What are the types of unicellular organisms?
Bioconjugation Chemical ReagentsCellular Structures and OrganellesNucleusPhysiological Probesbacteria


Posted on May 24, 2023
What types of cells can I sort?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the factors that affect the actual yield of the sorted cell samples?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What effect will the cell sorting process have on my cells' viability?
Chemical ReagentsCell ViabilityFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the techniques of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryCell Sorting Fluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What is the significance of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
How are cells separated?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What are the applications of cell sorting?
Chemical ReagentsFISHFlow Cytometry ReagentsFluorescenceImmunohistochemistry (IHC)Spectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
How do I prepare the samples for cell separation?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 10, 2023
What are the characteristics of dextran?
Bioconjugation Chemical Reagentsdextranlive cell tracking


Posted on May 10, 2023
What are the advantages of fluorophore-labeled dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the applications of dextran conjugates?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the degrees of labeling of the dextran conjugates?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the general properties of FITC-dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the general properties of TRITC-dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What factors affect the stability of dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
How do I dissolve dextran?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are labeled dextran conjugates used for?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
How are dextran conjugates purified?
Bioconjugation Chemical ReagentsPurificationdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the factors that can affect TRITC-dextran conjugation?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What is TRITC-dextran conjugate used for?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the factors that can affect dextran-FITC conjugation?
Bioconjugation Chemical Reagentsdextranlive cell analysislive cell tracking


Posted on May 10, 2023
What are the applications of dextran-FITC conjugation?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsMicroscopySpectral Flow Cytometrydextranlive cell analysislive cell tracking


Posted on May 9, 2023
What are the key measures of performance for cell separation methods?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
How does cell sorting work?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the differences between magnetic-activated cell sorting and FACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the pros of MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the mechanisms used to label cells in MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 2, 2023
What are the components in the sample loading buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What is a pH scale in chemistry?
Chemical ReagentsFluoresceinsIntracellular pHPhysiological Probes


Posted on April 26, 2023
What's the importance of pH in biological systems?
Chemical ReagentsClassic DyesFluoresceinsIntracellular pHPhysiological Probes


Posted on April 26, 2023
What is the pH for an acidic substance?
Chemical ReagentsClassic DyesFluoresceinsIntracellular pHPhysiological Probes


Posted on April 26, 2023
What materials are needed to make a phosphate buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
Why is a phosphate buffer solution especially useful for biological applications?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What criteria should I use to determine the type of lysis buffer required for an experiment?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What are the common lysis buffers for general protein extraction?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
How do I determine the concentration of an unknown acid or base solution?
Chemical ReagentsBuffer Preparations and Recipesbuffer


Posted on April 26, 2023
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 14, 2023
How do I identify pure substances and mixtures?
Chemical ReagentsStock SolutionWorking Solution


Posted on April 14, 2023
How do I know whether a substance is pure or impure?
Chemical ReagentsStock SolutionWorking Solution


Posted on April 14, 2023
Can homogeneous mixtures be separated?
Chemical ReagentsBuffer Preparations and RecipesStock SolutionWorking Solution


Posted on April 14, 2023
How do I separate mixtures and solutions?
Chemical ReagentsBuffer Preparations and RecipesStock SolutionWorking Solution


Posted on March 28, 2023
What are the differences between mixture and solution?
Chemical Reagents


Posted on March 28, 2023
How are mixtures classified?
Chemical ReagentsStock SolutionWorking Solution


Posted on March 28, 2023
Are solutions homogeneous?
Chemical ReagentsStock SolutionWorking Solutionbuffer


Posted on March 28, 2023
What are the types of solutions?
Chemical Reagents


Posted on March 28, 2023
What are the properties of a solution?
Chemical Reagents


Posted on March 28, 2023
What are the most hazardous solvents?
Chemical Reagents


Posted on March 28, 2023
What are organic solvents made of?
Chemical Reagents


Posted on March 28, 2023
What are the examples of solvent?
Chemical Reagents


Posted on March 28, 2023
Is water a solute or solvent?
Chemical Reagents


Posted on March 28, 2023
What are the differences between solute and solvent?
Chemical Reagents


Posted on March 20, 2023
What are the differences between disinfection and sterilization?
Chemical ReagentsCell CultureCell culture contaminationbacteriacell lineschemical contamination


Posted on March 17, 2023
What are the different types of mixture based on the size of the particles involved?
Chemical ReagentsBuffer Preparations and RecipesStock Solutionbuffer


Posted on March 17, 2023
What are the factors that affect solubility?
Chemical Reagents ToolsPhysiological Probes


Posted on March 17, 2023
How does temperature affect solubility?
Chemical Reagents ToolsPhysiological Probes


Posted on March 17, 2023
What causes solubility?
Chemical Reagents ToolsPhysiological Probes


Posted on March 17, 2023
What is a solubility test?
Chemical Reagents ToolsPhysiological Probes


Posted on March 17, 2023
What are the differences between dissociation and solvation?
Chemical Reagents ToolsPhysiological Probes


Posted on March 17, 2023
What are the differences between solution and suspension?
Chemical Reagents ToolsPhysiological ProbesStock Solution


Posted on March 17, 2023
What are the differences between solution and colloid?
Chemical Reagents ToolsPhysiological ProbesStock Solution


Posted on March 17, 2023
What are the types of colloids?
Chemical ReagentsPhysiological Probes


Posted on March 17, 2023
What are the differences between lipophilic and hydrophilic?
Chemical Reagents ToolsPhysiological Probes


Posted on February 14, 2023
Why is the acidity of phenols is higher in comparison to alcohols?
Chemical Reagents


Posted on February 14, 2023
What are the physical properties of phenol acidity?
Chemical Reagents


Posted on February 14, 2023
What are the factors that affect the acidity of phenols?
Chemical Reagents


Posted on February 14, 2023
What are the differences between phenol and cyclohexanol?
Chemical Reagents


Posted on February 3, 2023
How do I speed up a reaction?
Chemical ReagentsCell MetabolismEnzymesPhysiological Probes


Posted on January 30, 2023
What are the properties of glycine?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 27, 2023
Is glycine a weak acid?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 27, 2023
What are the differences between glycine and glycerin?
Chemical Reagents


Posted on January 27, 2023
What are the similarities between glycine and glycerin?
Chemical Reagents


Posted on January 27, 2023
What is the simplest amino acid?
Chemical Reagents


Posted on January 27, 2023
Why is glycine so important? (biology)
Chemical Reagents


Posted on January 27, 2023
What are the functions of glycine?
Chemical Reagents


Posted on January 27, 2023
What is glycine made of?
Chemical Reagents


Posted on January 27, 2023
What are the uses of glycine?
Chemical Reagents


Posted on January 27, 2023
What are the differences between magnesium glycinate and magnesium gluconate?
Chemical Reagents


Posted on January 25, 2023
What are the factors affecting fluorescence?
Chemical ReagentsClassic DyesPhysiological Probes


Posted on January 18, 2023
What is the structure of DMSO?
Chemical ReagentsDMSO


Posted on January 13, 2023
What are the differences between bioluminescence and fluorescence?
Bioconjugation Chemical ReagentsEnzymesFluorescenceLuciferasefluorescence imagingluminescense


Posted on January 13, 2023
What are the types of molecules that exhibit fluorescence?
Bioconjugation Chemical ReagentsCoumarinsCyaninesFluoresceinsFluorescenceRhodamines and Rhodamine Derivativesfluorescence imaging


Posted on January 13, 2023
What should I consider when choosing a fluorescence spectrometer?
Chemical ReagentsFluorescenceSpectral Flow Cytometryemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the different types of spectrometers used in fluorometry?
Bioconjugation Chemical ReagentsFluorescenceemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the differences between absorbance and fluorescence?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 10, 2023
What types of proteins are produced by attached ribosomes?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What types of proteins are produced by free ribosomes?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
Where are free ribosomes located?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What is the significance of free ribosomes?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What are the differences between free and attached ribosomes?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What are the similarities between free and attached ribosomes?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What are the differences between ribosomes and endoplasmic reticulum?
Chemical ReagentsCellular Structures and OrganellesEndoplasmic Reticulum (ER)OrganellesPhysiological ProbesProtein


Posted on January 10, 2023
What converts mRNA into a protein?
Chemical ReagentsCellular Structures and OrganellesOrganellesPhysiological ProbesProtein


Posted on January 9, 2023
What is the role of ATP in active transport?
Chemical ReagentsATPCell Proliferation AssaysCell SignalingCellular Structures and OrganellesEnzymesMembrane MarkerPlasma membrane


Posted on January 6, 2023
What are the MMPs enzymes in cells?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingEnzymesMMPProtein


Posted on January 6, 2023
What are the functions of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell SignalingPhysiological ProbesProtein


Posted on January 6, 2023
What is the structure of proteoglycans?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingProtein


Posted on January 6, 2023
What are the types of structural proteins in the extracellular matrix and their functions?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCellular Structures and OrganellesPhysiological Probes


Posted on December 14, 2022
What is atomic mass?
Chemical ReagentsAntibody and Protein LabelingPhysiological Probes


Posted on December 13, 2022
What are the differences between atoms and molecules?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the examples of an atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Can a molecule have only one atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is a simple molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
How do atoms become molecules?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Why can't we see an atom with the naked eye?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the limitations of Dalton's atomic theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are the general principles of Dalton's atomic theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is the basis for Dalton’s Atomic Theory?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What are atoms made of?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
What is the size of an atom?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Is salt a molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 13, 2022
Is oxygen a molecule?
Bioconjugation Chemical ReagentsCell Metabolism


Posted on December 8, 2022
What are the factors that will affect the rate and effectiveness of tissue fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the types of fixatives?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the general types of fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the commonly used chemical fixatives for microscopy?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the functions of fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the properties of an ideal fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What are the differences between formalin and formaldehyde?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Is formaldehyde a crosslinking agent?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
Why do tissues require fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on December 8, 2022
What is the purpose of cell fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells


Posted on November 30, 2022
What would happen without mitosis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
What is binary fission?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesNucleusPhysiological Probes


Posted on November 30, 2022
How does binary fission work?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
What is cytokinesis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 30, 2022
When does cytokinesis occur?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
How is cytokinesis different in plants and animals?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
When is cytokinesis complete?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
How many times does cytokinesis occur in meiosis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 30, 2022
What is the purpose of mitosis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesPhysiological Probes


Posted on November 21, 2022
How does the cell membrane protect the cell?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
How does the cell membrane change over time?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
Does the cell membrane allow something to move out of the cell?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
What molecules prevent cell membranes from dissolving?
Chemical ReagentsCell Plasma Membrane probesPhysiological ProbesPhysiological probesPlasma Membrane


Posted on November 21, 2022
What is the structure of the cell membrane?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
What organelles have two membranes?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
Is the cell membrane hydrophobic?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
Which part of the cell membrane is hydrophobic?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
What organelles does a plant cell have but an animal cell doesn't?
Bioconjugation Chemical ReagentsCellular ProcessesCellular Structures and OrganellesPhysiological Probes


Posted on November 21, 2022
What organelles have their own DNA and ribosomes?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesMitochondriaPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
What cellular components are common to prokaryotic and eukaryotic cells?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesPhysiological ProbesPlasma Membrane


Posted on November 21, 2022
What are the functions of intermediate filaments?
Bioconjugation Chemical ReagentsCellular ProcessesCellular Structures and Organelles


Posted on November 21, 2022
What are the functions of actin filaments?
Bioconjugation Chemical ReagentsActinCellular ProcessesCellular Structures and OrganellesMitochondriaPhysiological Probes


Posted on November 16, 2022
What are the single membrane organelles?
Chemical ReagentsCellular Structures and OrganellesClassic DyesEndoplasmic Reticulum (ER)Golgi ApparatusLysosomesPhysiological Probes


Posted on November 16, 2022
What are cellular membranes made of?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesClassic DyesPhysiological ProbesPlasma Membrane


Posted on November 16, 2022
What is the largest membrane-bound organelle in eukaryotic cells?
Chemical ReagentsCellular Structures and OrganellesClassic DyesNucleic AcidsNucleusPhysiological Probes


Posted on November 16, 2022
What are the similarities between cell organelles and cell inclusions?
Chemical ReagentsCellular Structures and OrganellesClassic DyesPhysiological ProbesPlasma Membrane


Posted on November 16, 2022
What are the differences between cell organelles and cell inclusions?
Chemical ReagentsCellular Structures and OrganellesClassic DyesEndoplasmic Reticulum (ER)Golgi ApparatusLysosomesMitochondriaNucleusPhysiological Probes


Posted on November 16, 2022
What are the general features of inclusion bodies?
Chemical ReagentsCellular Structures and OrganellesClassic DyesOrganellesPhysiological Probes


Posted on November 16, 2022
Can ribosomes be considered inclusion bodies?
Chemical ReagentsCellular Structures and OrganellesClassic DyesOrganellesPhysiological Probes


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What are the differences between GFP and YFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What activates GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the applications of GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingChemical ReagentsClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What's the relationship between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the differences between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the types of fluorophores?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic DyesFluoresceinsProteinRhodamines and Rhodamine Derivatives


Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
How are FITC signals detected?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFluoresceinsImmunohistochemistry (IHC)amine-reactiveimmunocytochemistry


Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
How do I dissolve a FITC-dextran?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
Is FITC soluble in water?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What does FITC bind to?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization


Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence


Posted on November 8, 2022
Are all zwitterions pH neutral?
Chemical ReagentsBioconjugationClassic DyesDye to Peptide Conjugation


Posted on November 7, 2022
Why are Annexin V conjugates and 7-AAD used together?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesClassic DyesFluoresceinsNucleusannexin V


Posted on November 1, 2022
What are the functions of actin based thin filaments?
Bioconjugation Chemical ReagentsActinCell Structures and OrganellesMitochondria


Posted on October 24, 2022
What are the different types of ligands?
Chemical ReagentsCell Structures and OrganellesClassic DyesNucleusPhysiological Probes


Posted on October 14, 2022
Are coumarins water-soluble?
Chemical ReagentsBioconjugationClassic DyesCoumarins


Posted on October 14, 2022
Are coumarins useful for immunofluorescence?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)BioconjugationClassic DyesCoumarins


Posted on October 14, 2022
Are coumarin dyes pH sensitive?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What color do coumarins fluoresce?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What are the applications of coumarin dyes?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What does 'coumarin nucleus' mean?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Is coumarin a carcinogen?
Chemical ReagentsBioconjugationClassic DyesCoumarins


Posted on October 7, 2022
What are the differences between intracellular and intercellular signaling?
Chemical ReagentsCell Cycle AnalysisCellular ProcessesPhysiological Probes


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 16, 2022
Why is it important for cells to divide?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
What are the types of cell division?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
How do cells divide?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
What are the phases of a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
Is interphase part of mitosis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
Where does DNA replication occur?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesDNA


Posted on September 16, 2022
Is the chromosome number the same in the daughter cells as it was in the parent cell?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
What is the shortest phase of a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
How long does the cell cycle take?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
Which organisms perform mitosis?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Processes


Posted on September 16, 2022
Do mitochondria produce ATP?
Chemical ReagentsATPCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesEnergyMitochondriamitochondria membrane potential


Posted on September 16, 2022
Why is the number of ATP not known exactly?
Chemical ReagentsATPCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesEnergy


Posted on September 16, 2022
What are the examples of energy storage molecules?
Chemical ReagentsATPCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesEnergy


Posted on September 16, 2022
How do I choose an ATP Assay?
Chemical ReagentsATPCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesEnergy


Posted on September 16, 2022
How does the ATP bioluminescence assay work?
Chemical ReagentsATPCellular ProcessesD-LuciferinLuciferasechemiluminescencefirefly luciferaseluciferinluminescense


Posted on September 16, 2022
What are the advantages of the ATP bioluminescence assays?
Chemical ReagentsCellular ProcessesD-LuciferinLuciferasechemiluminescencefirefly luciferaseluciferinluminescense


Posted on September 16, 2022
What substrates are used for the ATP bioluminescence assays?
Chemical ReagentsATPCellular ProcessesD-LuciferinLuciferasechemiluminescencefirefly luciferaseluciferinluminescense


Posted on September 16, 2022
Where is ATP made in the cell?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyMitochondria


Posted on September 13, 2022
What are the examples of channel proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What is the function of channel proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What is the structure of channel proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What are the functions of the carrier proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What are the examples of carrier proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What is the mechanism of carrier proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What are the types of channel proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesProteinion channelmembrane proteins


Posted on September 13, 2022
What are the differences between channel and carrier proteins?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular Ionsion channel


Posted on September 13, 2022
What are the functions of ATP?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probesion channel


Posted on September 13, 2022
What is ATP made of?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probes


Posted on September 13, 2022
How is ATP used?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probes


Posted on September 13, 2022
How does ATP work?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probes


Posted on September 13, 2022
What are the differences between cyclic AMP and AMP?
Chemical ReagentsATPCell SignalingCellular ProcessesIntracellular IonsIntracellular ProbesSecond Messenger


Posted on September 13, 2022
What are the similarities between cyclic AMP and AMP?
Chemical ReagentsCell SignalingCellular ProcessesIntracellular IonsIntracellular Probes


Posted on September 13, 2022
How is ATP produced from AMP or ADP?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyEnzymesIntracellular IonsIntracellular Probes


Posted on September 13, 2022
How is energy stored in ATP?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probes


Posted on September 13, 2022
What is produced when ATP is hydrolyzed?
Chemical ReagentsATPCell SignalingCellular ProcessesEnergyIntracellular IonsIntracellular Probes


Posted on August 30, 2022
What are the principles of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 30, 2022
What are the steps of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 30, 2022
What are the uses of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical Reagentsspectrophotometer


Posted on June 2, 2022
What assays should I use to determine total protein content?
Chemical ReagentsCellular ProcessesPhysiological ProbesAntibody and Protein Labeling


Posted on June 2, 2022
What is the difference between Tide Quencher 2 (TQ2) and Tide Quencher 2WS (TQ2WS) products?
Tide Quencher Dyes Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingAntibody and Protein Labeling Chemical Reagents


Posted on November 14, 2020
How do you purify oligonucleotide conjugates by HPLC?
Bioconjugation Chemical Reagents Peptide and Oligonucleotide LabelingConjugationPeptide and Oligonucleotide


Posted on September 29, 2020
What is the difference between Western Blot and ELISA?
Antibodies and Proteomics Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on July 22, 2020
How can I dissociate adherent cells?
Antibodies and Proteomics Chemical ReagentsCell Cycle and ProliferationEnzymesadherent cells


Posted on July 22, 2020
What is calcein AM?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes


Posted on July 22, 2020
What does calcein AM stain?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes


Posted on July 22, 2020
How does calcein AM work?
Chemical ReagentsCalcein AMCell ViabilityCell Viability AssaysCellular ProcessesFluoresceinsGreen FluorescencePhysiological Probes


Posted on July 22, 2020
How does DAPI work?
Antibodies and Proteomics Bioconjugation Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesDAPI


Posted on July 22, 2020
How does trypan blue work?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on July 15, 2020
6-ROX is predominately used as a reference dye for performing PCR detections, however, 6-ROX is very unstable compared to other rhodamine dyes. What is a better dye replacement?
Chemical ReagentsDNA and RNADNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
How does alcohol denature a protein?
Antibodies and Proteomics Chemical ReagentsProtein


Posted on June 1, 2020
Why is fluorescence more sensitive than UV-VIS absorption spectroscopy?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on June 1, 2020
What is the mitosis phase in a cell cycle and what are the phases of mitosis phase?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
How long is the duration of each phase in a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
How does BrdU labeling work?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the role/function of SDS in SDS-PAGE?
Chemical Reagentsdetergent


Posted on June 1, 2020
What is the difference between cell proliferation and cell growth?
Chemical ReagentsCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesClassic Dyescell proliferation


Posted on June 1, 2020
What is cell division and types?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
What are cell cycle and cell cycle phases?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on May 18, 2020
What is FSC and SSC in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
Why does trypan blue quench FITC or GFP in flow cytometry?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is trypan blue (TB) assay?
Chemical ReagentsCell ViabilityCell Viability AssaysImmunohistochemistry (IHC)Live or Deadcell-impermeable


Posted on May 18, 2020
Are there upgraded trypan blue derivatives for cell viability testing?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesFluoresceinsFluorescencePhysiological Probes


Posted on May 18, 2020
What is cell viability assay?
Antibodies and Proteomics Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesImmunohistochemistry (IHC)


Posted on May 18, 2020
What is fluorescent activated cell sorting (FACS)?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What are the beneficial activities of reactive oxygen species (ROS)?
Chemical ReagentsCellular MetabolismCellular ProcessesPhysiological ProbesROSROS ProbesReactive Oxygen Species (ROS)


Posted on May 18, 2020
How do DNA binding dyes bind to DNA?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Physiological ProbesPropidium iodide (PI)TUNEL


Posted on May 18, 2020
What is endosomal pH?
Chemical ReagentsCellular ProcessesCellular Structures and OrganellesEnzymesIntracellular pHPhysiological ProbespH probes


Posted on May 18, 2020
What's the difference between an early and late endosome?
Chemical ReagentsCellular ProcessesCellular Structures and OrganellesEnzymesIntracellular pHPhysiological ProbespH probes


Posted on May 18, 2020
What is the function of chloroform in RNA extraction?
Chemical ReagentsCellular Structures and OrganellesDNA and RNAFlow Cytometry ReagentsPurificationRNASpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is difference between therapeutic and prophylactic group in research?
Antibodies and Proteomics Chemical ReagentsImmunohistochemistry (IHC)


Posted on May 18, 2020
How do you detect apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 18, 2020
How do you identify stages of apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCellular ProcessesCellular Structures and OrganellesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 18, 2020
What does EDTA do in agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisChelatorEDTAGel Electrophoresisbuffer


Posted on May 18, 2020
How do you evaluate the transfection efficiency?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 18, 2020
What is the difference between transient and stable transfection?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 18, 2020
What transfection methods are commonly used in biology?
Chemical ReagentsDNA and RNALipofectamineLipofectamine 2000Lipofectamine 3000Nucleic AcidsTransfectionTransfection Reagents


Posted on May 18, 2020
What is the difference between Side Scatter Pulse Width (SSC-w) and Side Scatter Pulse Height (SSC-h)?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometrymultiplex


Posted on May 9, 2020
What is next-generation sequencing (NGS)?
Chemical ReagentsDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?
Chemical ReagentsAV-FITCAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesPropidium iodide (PI)annexin V


Posted on May 9, 2020
What is the function of DMSO in PCR?
Chemical ReagentsDMSOPCRPolymerase Chain Reaction (PCR)


Posted on May 9, 2020
Can apoptosis be detected by cell cycle studies?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesTUNELannexin V


Posted on May 9, 2020
What is the function of MgCl2 in PCR?
Chemical ReagentsDNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Polymerasesmagnesium


Posted on May 9, 2020
Which probe should I use to evaluate change in membrane potential, JC-1 or TMRE?
Chemical ReagentsCellular ProcessesClassic DyesJC-1JC-10Membrane Potential and ChannelsMitochondriamitochondria membrane potential


Posted on May 9, 2020
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer


Posted on May 9, 2020
Which viability test is better: MTT or trypan blue?
Chemical ReagentsCell Cycle and ProliferationCell CytotoxicityCell Membrane IntegrityCell Proliferation AssaysCell ViabilityCell Viability AssaysLive or Dead


Posted on May 9, 2020
Can mitochondrial tracking probes be used with flow cytometry?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsMitochondriaSpectral Flow Cytometryflow cytometrymitochondria membrane potential


Posted on May 9, 2020
What methods are best for stimulating intracellular calcium release?
Chemical ReagentsCalcium IndicatorsCell SignalingCellular ProcessesPhysiological Probes


Posted on May 9, 2020
What methods are best for inhibiting intracellular calcium release?
Chemical ReagentsCalcium IndicatorCell SignalingCellular ProcessesChelatorEGTAPhysiological Probes


Posted on May 9, 2020
What is the effect of phenol red in DMEM (Dulbecco’s Modified Eagle Medium)?
Chemical ReagentsCell CultureIHCImmunohistochemistry (IHC)


Posted on May 9, 2020
What is the difference between nuclease-free water and ultrapure water?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic Acids


Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex


Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent


Posted on April 24, 2020
What is the difference between staining buffer and FACS buffer?
Chemical ReagentsBSABuffer Preparations and RecipesEDTAImmunohistochemistry (IHC)PBSbuffer


Posted on April 24, 2020
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence


Posted on April 24, 2020
Why might polyclonal antibodies give better results than monoclonal antibodies?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Physiological Probes


Posted on April 24, 2020
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer


Posted on April 24, 2020
What is the difference between antibiotics and antibacterials?
Chemical Reagentsbacteriainhibitor


Posted on April 24, 2020
What is the difference between silica gels and bare silica?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAffinity ChromatographyChromatographic techniques


Posted on April 24, 2020
How do you dilute primary antibodies from 1:200 to 1:1000?
Chemical ReagentsBuffer Preparations and RecipesCalculating ConcentrationIHCImmunohistochemistry (IHC)Serial Dilutionbuffer


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
Is it okay to stain cells for both intercellular and surface markers for FACS analysis?
Chemical ReagentsCell Cycle AnalysisCellular ProcessesCellular Structures and OrganellesFixationPermeabilizationPhysiological Probes


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 24, 2020
What is in antiserum?
Antibodies and Proteomics Bioconjugation Chemical ReagentsProtein


Posted on April 24, 2020
What is the difference between acetone dehydration and ethanol dehydration?
Chemical Reagentsethanol


Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
How does PBS clean cells?
Chemical ReagentsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)PBSbuffer


Posted on April 17, 2020
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer


Posted on April 17, 2020
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer


Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization


Posted on April 17, 2020
Why is 100% Ethanol used in rehydration of paraffin embedded tissues?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Paraffin embedded sectionsProtein


Posted on April 17, 2020
Does PBS buffer have any effect on cell adhesion?
Buffer Preparations and RecipesPBSadherent cellsbuffer Chemical Reagents


Posted on April 17, 2020
Techniques for storage of tissues post-fixation?
FixationStoragefixed cells Chemical ReagentsImmunohistochemistry (IHC)


Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent


Posted on April 17, 2020
How can I attach suspension cells to glass slides for fluorescence microscopy investigation?
FixationPBSfixed cellsfluorescence microscope Chemical Reagents


Posted on April 17, 2020
What is the difference between PLL and PDL for surface coating?
Cell CultureFixationadherent cellsfixed cells Chemical Reagentsfluorescence microscope


Posted on April 17, 2020
Why do we use Citrate buffer in Antigen retrieval step during IHC?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)buffer Chemical ReagentsBuffer Preparations and Recipes


Posted on April 17, 2020
When should DTT be added in tissue lysis?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesPhysiological Probesbuffer


Posted on April 17, 2020
Which is the best conditions for methanol fixation of microtubules?
Bioconjugation Chemical ReagentsClassic DyesFixationPhysiological Probesfixed cells


Posted on April 17, 2020
What is blocking buffer in IHC?
Antibodies and ProteomicsBSAImmunohistochemistry (IHC)PBSbuffer Chemical ReagentsIHC


Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer


Posted on April 17, 2020
Is Gelatin or BSA best for blocking in immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAimmunofluorescence Chemical Reagents


Posted on April 17, 2020
What is the role of sodium azide in flow cytometry?
Antibodies and Proteomics Chemical ReagentsFlow Cytometry ReagentsFluorescencePhysiological ProbesSpectral Flow Cytometry


Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent


Posted on April 17, 2020
What is the exact role of NH4Cl (ammonium chloride) in aqueous barbier type reactions?
Chemical Reagents


Posted on April 17, 2020
What are the differences between stable and permanent transfection/transduction?
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic AcidsPhysiological ProbesTransfectionTransfection Reagents


Posted on April 17, 2020
What is the purpose of a buffered solution used for RNase treatment?
Chemical ReagentsBuffer Preparations and RecipesDNA PurityDNA and RNAEnzymesNucleic Acidsbuffer


Posted on April 17, 2020
What is the purpose of using cold PBS in the washing step of Annexin V staining?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell MetabolismCell ViabilityCellular ProcessesPBSannexin V


Posted on April 17, 2020
What % of BSA to use for blocking step?
Antibodies and Proteomics Chemical ReagentsBSABuffer Preparations and RecipesPhysiological Probesbovine serum albuminbuffer


Posted on April 7, 2020
What rhodamine dye do you have for automated DNA sequencing and nucleotide labeling?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesDNA sequencingFluoresceinsRhodamines and Rhodamine DerivativesPhysiological Probes


Posted on April 7, 2020
Share several ways of labeling an oligonucleotide with fluorescein like 6-FAM/6-HEX/6-TET phosphoramidite?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesFluoresceinsPhysiological Probes


Posted on April 2, 2020
What is the role of magnesium in cell metabolism?
Chemical ReagentsCellular MetabolismCellular ProcessesClassic DyesFluoresceinsmagnesium