FAQ

Posted on February 2, 2023
Do ribosomes contain DNA?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the differences between 70s and 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the similarities between 70s ribosomes and 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the subunits of 70s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the subunits of 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the binding sites on ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
How do eukaryotic ribosome binding sites work?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
What are the differences between Shine Dalgarno and Kozak Sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on February 2, 2023
Where is the shine dalgarno sequence found?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation

Posted on January 20, 2023
What are the important features required for a plasmid vector?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus

Posted on December 13, 2022
Why do 60S & 40S ribosomal subunits make an 80S ribosome instead of the 100S?
BioconjugationCell Structures and OrganellesMitochondriaNucleus

Posted on December 13, 2022
Which organelle is known as the protein factory of the cell?
BioconjugationCell Structures and OrganellesMitochondria

Posted on November 19, 2022
How do lipids get in the plasma membrane?
BioconjugationCell Structures and OrganellesMitochondriaPlasma Membrane

Posted on November 17, 2022
How do I stain cells with propidium iodide?
Apoptosis and NecrosisBioconjugationCell Cycle AssaysCell Proliferation AssaysCell Viability AssaysCellular ProcessesNucleus

Posted on November 16, 2022
What are the similarities between MTT and MTS assays?
BioconjugationCell Proliferation AssaysCell Viability AssaysCellular Processes

Posted on November 16, 2022
What are the limitations of MTT assays?
BioconjugationCell Cycle AssaysCell Proliferation AssaysCell Viability AssaysCellular Processes

Posted on November 16, 2022
Can DAPI stain dead cells?
Antibody and Protein LabelingBioconjugationCell Cycle AssaysCell CytotoxicityCell Proliferation AssaysCell Structures and OrganellesCellular Processes

Posted on November 10, 2022
Which metabolic pathway produces the most ATP?
BioconjugationCell Proliferation AssaysCell SignalingCellular ProcessesEnzymesPolymerases

Posted on November 10, 2022
What are the similarities between photosynthesis and cellular respiration?
BioconjugationCell Proliferation AssaysCellular ProcessesEnzymesPolymerases

Posted on November 10, 2022
What is the significance of ATP?
BioconjugationCell Proliferation AssaysCellular ProcessesDNA and RNA QuantitationEnzymesPolymerases

Posted on November 9, 2022
Where is ATP produced?
BioconjugationCell ProliferationCellular ProcessesEnzymesPolymerases

Posted on November 9, 2022
What are the similarities between B cell receptor and antibody?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationCellular Processes

Posted on November 8, 2022
Are all zwitterions pH neutral?
Chemical ReagentsBioconjugationClassic DyesDye to Peptide Conjugation

Posted on November 8, 2022
What kind of washing buffer should I use for immunoprecipitation protocols?
ToolsBioconjugationBuffer Preparations and Recipes

Posted on November 4, 2022
What are the types of myofilaments?
ActinBioconjugationCell Proliferation AssaysCell Structures and OrganellesCellular Processes

Posted on November 4, 2022
What is myosin made of?
ActinBioconjugationCell Proliferation AssaysCell Structures and OrganellesCellular Processes

Posted on November 4, 2022
Where is myosin found?
ActinBioconjugationCell Proliferation AssaysCell Structures and OrganellesCellular ProcessesMitochondria

Posted on November 4, 2022
Do microfilaments have polarity?
ActinBioconjugationCell Structures and OrganellesCellular ProcessesMitochondria

Posted on November 4, 2022
Is ATP required to provide energy for actin polymerization?
ActinBioconjugationCell SignalingCellular ProcessesEnzymesCell Proliferation

Posted on November 4, 2022
How do actin filaments form?
Antibody and Protein LabelingBioconjugationCell Structures and OrganellesPhalloidin Conjugates

Posted on October 31, 2022
What are the similarities between G actin and F actin?
ActinAntibody and Protein LabelingBioconjugationCell Structures and OrganellesMitochondriaPhalloidin Conjugates

Posted on October 26, 2022
Is MTT cell permeable?
BioconjugationCell Cycle AssaysCell Proliferation AssaysCell Structures and OrganellesCell Viability AssaysCellular Processes

Posted on October 26, 2022
What does trypan blue do?
BioconjugationCell Structures and OrganellesCell Viability AssaysCellular Processes

Posted on October 26, 2022
Why is DMSO added in MTT assay?
BioconjugationCell Cycle AssaysCell Proliferation AssaysCell Structures and OrganellesCell Viability AssaysNucleus

Posted on October 24, 2022
What are the functions of receptors?
BioconjugationCell Cycle AssaysCell MetabolismCell Structures and OrganellesCellular Processes

Posted on October 22, 2022
What are the similarities between RNA splicing and alternative splicing?
BioconjugationCell Structures and OrganellesDNA and RNA Quantitation

Posted on October 22, 2022
What are the major steps involved in RNA processing?
BioconjugationCell Structures and OrganellesDNA and RNA QuantitationDNA and RNA Quantitation

Posted on October 22, 2022
What are the differences between a linear and crosslinked polymer?
BioconjugationProtein to Protein ConjugationBuccutite Crosslinkers and Kits

Posted on October 20, 2022
What are the differences between epithelial and endothelial cells?
BioconjugationCell Structures and OrganellesCellular Processes

Posted on October 19, 2022
What are the differences between organs and organelles?
BioconjugationCell Structures and OrganellesCellular ProcessesMitochondria

Posted on October 19, 2022
What are the differences between plasma membrane and cell membrane?
BioconjugationCell Structures and OraganellesMitochondriaPlasma Membrane

Posted on October 18, 2022
Do lysosomes contain DNA?
BioconjugationCell Structures and OraganellesLysosomesMitochondria

Posted on October 18, 2022
What happens if there are no mitochondria in a eukaryotic cell?
BioconjugationCell Proliferation AssaysCell Structures and OrganellesEnzymesMitochondriaATP

Posted on October 14, 2022
What are the types of lysosomes?
BioconjugationCell Structures and OrganellesCellular ProcessesCellular Structures and OrganellesLysosomes

Posted on October 14, 2022
What does acridine orange stain?
BioconjugationCell Cycle AssaysCell Structures and OrganellesCellular ProcessesDNA and RNA QuantitationNucleus

Posted on October 14, 2022
Is propidium iodide cell permeable?
Antibodies and ProteomicsBioconjugationCell Cycle AssaysCell Structures and Organelles

Posted on October 14, 2022
What are the common types of microscope light sources?
Antibodies and ProteomicsBioconjugationNucleusPhysiological Probes

Posted on October 14, 2022
Is coumarin legal?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Where does coumarin come from?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Are coumarins water-soluble?
Chemical ReagentsBioconjugationClassic DyesCoumarins

Posted on October 14, 2022
Is coumarin a natural chemical?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Is there a better dye than AMCA?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
What are coumarin derivatives used for?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Are coumarins useful for immunofluorescence?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)BioconjugationClassic DyesCoumarins

Posted on October 14, 2022
What is coumarin used for?
BioconjugationChemical ReagentsClassic DyesCoumarins

Posted on October 14, 2022
Is coumarin a carcinogen?
Chemical ReagentsBioconjugationClassic DyesCoumarins

Posted on October 14, 2022
What are the principles of IR spectroscopy/vibrational spectroscopy?
Antibody and Protein LabelingAntibody and ProteomicsBioconjugationClassic Dyes

Posted on October 13, 2022
Where are ribosomes located?
BioconjugationCell Structures and OrganellesDNA and RNA QuatitationEndoplasmic Reticulum (ER)

Posted on October 13, 2022
What are ribosomes?
BioconjugationCell Structures and OrganellesDNA and RNA Quatitation

Posted on October 7, 2022
What are the differences between polyclonal and monoclonal antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on October 7, 2022
What are recombinant antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on October 7, 2022
What are the advantages of polyclonal antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on September 1, 2022
What are the purposes of a fluorescent marker?
BioconjugationCell Structures and OrganellesCell Viability AssaysCellular ProcessesClassic DyesCluster of Differentiation (CD Antibodies)Cyanines

Posted on September 1, 2022
What are the similarities between flow cytometry and immunohistochemistry?
BioconjugationCell Cycle AssaysCell Structures and OrganellesCellular ProcessesFlow Cytometry ReagentsImmunohistochemistry (IHC)

Posted on September 1, 2022
What are the differences between emission spectrum and absorption spectrum?
BioconjugationTools

Posted on September 1, 2022
What are the major differences between reporter genes and selectable markers?
Amplite® Reagents and Assay KitsBioconjugationEnzymesLuciferases

Posted on August 31, 2022
What is a crosslinked polymer?
Antibody and Protein LabelingBioconjugationPolymerase Chain Reaction (PCR)PolymerasesProtein to Protein Conjugation

Posted on August 30, 2022
What is the structure of epithelial cells?
BioconjugationCell Structures and OrganellesCellular ProcessesGolgi ApparatusMitochondria

Posted on August 30, 2022
How are epithelial tissues classified?
Antibodies and ProteomicsBioconjugationCell Structures and OrganellesCell Viability AssaysCellular Processes

Posted on August 29, 2022
What are the differences between immunoglobulin and antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on August 26, 2022
What are the similarities between epithelial and endothelial cells?
BioconjugationCell Structures and OrganellesCellular Processes

Posted on August 26, 2022
What are the similarities between tissues and organs?
BioconjugationCell Structures and OrganellesCell Viability AssaysCellular Processes

Posted on August 26, 2022
Which is the largest organelle in a cell?
BioconjugationCell Structures and OrganellesCell Viability AssaysCellular ProcessesDNA and RNA QuantitationNucleus

Posted on August 26, 2022
What are the differences between cells and tissues?
BioconjugationCell Structures and OrganellesEndoplasmic Reticulum (ER)LysosomesMitochondria

Posted on August 26, 2022
What are the differences between tissues and organs?
BioconjugationCell Structures and OrganellesCell Viability Assays

Posted on August 26, 2022
What is the primary component of the cell wall?
BioconjugationCell Structures and OrganellesCellular ProcessesGram Staining

Posted on August 26, 2022
What is the composition of the plasma membrane?
BioconjugationCell Structures and OrganellesPlasma Membrane

Posted on August 26, 2022
Do all bacteria have plasmids?
BioconjugationCell Structures and OrganellesDNA and RNA QuantitationGram StainingNucleus

Posted on August 25, 2022
What are the differences between phage display and hybridoma?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on August 23, 2022
What is the purpose of the gram staining technique in microbiology?
BioconjugationCell Structures and OrganellesCellular PorcessesGram Staining

Posted on August 18, 2022
What are the processing steps for messenger RNAs?
BioconjugationDNA and RNADNA and RNA QuantitationTransfection Reagents

Posted on August 18, 2022
What are the common types of RNA editing?
DNA and RNA QuantitationCell Structures and OrganellesMitochondriaBioconjugationNucleus

Posted on August 18, 2022
What happens during RNA editing?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationBioconjugationMitochondria

Posted on August 17, 2022
What are the main types of antibodies?
Antibody and Protein LabelingAntibodies and ProteomicsImmunohistochemistry (IHC)Antibody and Protein LabelingBioconjugation

Posted on August 17, 2022
What should be considered when generating monoclonal antibodies?
BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Antibodies and ProteomicsPhysiological Probes

Posted on August 12, 2022
Do eukaryotic cells have a nucleus?
NucleusBioconjugation

Posted on August 5, 2022
What are the differences between flow cytometry and immunohistochemistry?
Flow Cytometry ReagentsImmunohistochemistry (IHC)BioconjugationAntibodies and ProteomicsProtein to Protein Conjugation

Posted on August 5, 2022
What are the differences between IgG, IgM, IgE and IgD?
Antibody and Protein LabelingSecondary AntibodiesImmunohistochemistry (IHC)Bioconjugation

Posted on August 3, 2022
What are the common reporter genes?
Cellular ProcessesCell MetabolismEnzymesBioconjugationPhysiological Probes

Posted on August 2, 2022
What are the differences between B cell receptor and antibody?
Antibody and Protein LabelingBioconjugationAntibodies and ProteomicsImmunohistochemistry (IHC)

Posted on August 1, 2022
Do all cells have a cell wall?
NucleusCell Structures and OrganellesBioconjugationCell Cytotoxicity

Posted on August 1, 2022
What are the similarities between plant cells and animal cells?
Cell Structures and OrganellesBioconjugationCell MetabolismCellular Processes

Posted on July 27, 2022
What are the disadvantages of polyclonal antibodies?
Cellular ProcessesAntibody and Protein LabelingBioconjugationAntibodies and ProteomicsCrosslinkers

Posted on July 27, 2022
How does fluorescent microscopy work?
BioconjugationCell Structures and OrganellesPhysiological ProbesNucleus

Posted on July 21, 2022
What are the applications of phage display technology?
BioconjugationChemical ReagentsAntibodies and ProteomicsAntibody and Protein Labeling

Posted on July 21, 2022
What are the types of combinatorial antibody libraries?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on July 21, 2022
What is a hybridoma cell used for?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics

Posted on July 21, 2022
What does phage display detect?
Antibody and Protein LabelingProtein to Protein ConjugationAntibodies and ProteomicsBioconjugation

Posted on July 21, 2022
What portions of the antibody gene should be cloned for the generation of recombinant antibodies?
BioconjugationAntibody and Protein Labeling

Posted on July 21, 2022
How are recombinant antibodies produced?
Antibody and Protein LabelingAntibodies and ProteomicsBioconjugation

Posted on July 15, 2022
What is the difference between membrane potential and equilibrium potential?
Membrane Potential and ChannelsBioconjugation

Posted on July 15, 2022
What is the difference between membrane potential and action potential?
Membrane Potential and ChannelsBioconjugation

Posted on July 15, 2022
How does a cell maintain resting membrane potential?
BioconjugationMembrane Potential and Channels

Posted on July 15, 2022
How does temperature affect equilibrium potential?
Membrane Potential and ChannelsBioconjugation

Posted on July 15, 2022
Which side of the cell membrane is positive?
BioconjugationCell Structures and ORganelles

Posted on July 12, 2022
What is the alternative to propidium iodide for dead cell staining?
Cell CytotoxicityCellular ProcessesBioconjugationFlow Cytometry ReagentsCell Cycle AssaysDNA and RNA Quantitation

Posted on June 30, 2022
What are polyclonal antibodies?
BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibodies and Proteomics

Posted on June 30, 2022
What are the advantages of using primary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibody and Protein LabelingBioconjugation

Posted on June 30, 2022
What are the advantages of using secondary antibodies?
Antibody and Protein LabelingBioconjugationEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibodies and Proteomics

Posted on June 30, 2022
What are secondary antibodies?
BioconjugationEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibody and Protein Labeling

Posted on June 30, 2022
What are primary antibodies?
Physiological ProbesAntibody and Protein LabelingEnzyme-Linked Immunosorbent Assay (ELISA)Bioconjugation

Posted on June 29, 2022
How are recombinant antibodies made?
Antibodies and ProteomicsDNA and RNA QuantitationAntibody and Protein LabelingBioconjugationPhysiological Probes

Posted on June 29, 2022
What are the applications of antibodies?
BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)

Posted on June 29, 2022
What are the common types of antibodies?
Immunohistochemistry (IHC)Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on June 29, 2022
How are monoclonal antibodies made?
Antibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)

Posted on June 28, 2022
What are the types of recombinant antibody fragments?
Antibody and Protein LabelingEnzyme-Linked Immunosorbent Assay (ELISA)Antibodies and ProteomicsImmunohistochemistry (IHC)Bioconjugation

Posted on June 28, 2022
How do I select the right secondary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Bioconjugation

Posted on June 27, 2022
Why do I need both primary and secondary antibodies in western blot?
Antibody and Protein LabelingBioconjugationCrosslinkersProtein to Protein Conjugation

Posted on June 27, 2022
What are the main differences between a primary antibody and a secondary antibody?
Antibody and Protein LabelingBioconjugationAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)

Posted on June 24, 2022
What are the common types of primary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBioconjugation

Posted on June 24, 2022
What are the most common types of secondary antibodies?
BioconjugationAntibodies and ProteomicsEnzymesEnzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling

Posted on June 24, 2022
What are the Similarities Between Primary and Secondary Antibody?
Physiological ProbesAntibody and Protein LabelingEnzyme-Linked Immunosorbent Assay (ELISA)Bioconjugation

Posted on June 24, 2022
How do I choose primary and secondary antibodies?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics

Posted on June 24, 2022
What are the two types of immunofluorescence?
Antibody and Protein LabelingImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Bioconjugation

Posted on June 23, 2022
What is whole genome sequencing?
BioconjugationCoronavirus (SARS-CoV-2)DNA and RNA QuantitationPhysiological ProbesNucleus

Posted on June 23, 2022
What factors affect protein stability?
Antibody and Protein LabelingProtein to Protein ConjugationBioconjugation

Posted on June 17, 2022
What is loop-mediated isothermal amplification?
BioconjugationCell Structures and OrganellesPhysiological ProbesNucleusDNA and RNA Quantitation

Posted on June 17, 2022
What are the components of cytoplasm?
NucleusPhysiological ProbesCell Structures and OrganellesCellular ProcessesBioconjugation

Posted on June 15, 2022
Where is the cytosol located in a cell?
Cell Structures and OrganellesBioconjugationPhysiological Probes

Posted on June 10, 2022
What is the cell wall made of?
Cell Structures and OrganellesCellular ProcessesCell ViabilityBioconjugation

Posted on June 10, 2022
What are the basic phases of mitosis?
Cell Structures and OrganellesCell Cycle AssaysBioconjugationCellular Processes

Posted on June 9, 2022
Do fungi have cell walls?
Cell ViabilityCell Structures and OrganellesNucleusEnzymesBioconjugation

Posted on June 9, 2022
Where is the cell wall located?
Cell Structures and OrganellesBioconjugationCell Viability

Posted on June 6, 2022
What are the examples of eukaryotic cells?
BioconjugationNucleusCellular ProcessesCell ViabilityDNA and RNA QuantitationPhysiological Probes

Posted on June 6, 2022
How does a eukaryotic cell divide?
Cell Structures and OrganellesCell Cycle AssaysPhysiological ProbesCell MetabolismCellular ProcessesBioconjugation

Posted on May 27, 2022
How does DNA replication relate to mitosis?
Cell Cycle AssaysCell Structures and OrganellesDNA and RNA QuantitationBioconjugation

Posted on May 25, 2022
How to choose the right method of crosslinking?
CrosslinkersBioconjugationChemical ReagentsProtein to Protein Conjugation

Posted on May 25, 2022
What is crosslinking?
Protein to Protein ConjugationBioconjugationCrosslinkersChemical Reagents

Posted on May 25, 2022
What is chemical cross-linking?
BioconjugationProtein to Protein ConjugationCrosslinkersChemical Reagents

Posted on May 25, 2022
What is physical cross-linking?
Protein to Protein ConjugationCrosslinkersBioconjugation

Posted on May 24, 2022
What is the difference between mass spectroscopy and mass spectrometry?
Classic DyesBioconjugationAntibodies and ProteomicsAntibody and Protein Labeling

Posted on May 20, 2022
What is the most important characteristic of eukaryotic cells that distinguishes it from prokaryotic cells?
BioconjugationNucleusCell Structures and OrganellesPlasma MembraneDNA and RNA Quantitation

Posted on May 20, 2022
What are the characteristics of prokaryotic cells?
Cell Structures and OrganellesBioconjugationNucleusPlasma MembraneDNA and RNA Quantitation

Posted on May 20, 2022
Are eukaryotic cells unicellular or multicellular?
Cell Structures and OrganellesNucleusBioconjugation

Posted on May 13, 2022
What are the components of prokaryotic cells?
Cell Structures and OrganellesLysosomesCell CytotoxicityCellular ProcessesBioconjugation

Posted on May 13, 2022
What are the differences between compound and mixture?
CrosslinkersBioconjugationPhysiological Probes

Posted on May 13, 2022
Is energy always released or absorbed during a chemical reaction?
EnzymesCell MetabolismCellular ProcessesPhysiological ProbesBioconjugation

Posted on May 13, 2022
What are the differences between endothermic and exothermic reactions?
Physiological ProbesBioconjugationCell Metabolism

Posted on May 13, 2022
How do I tell if it's a physical or chemical change?
Classic DyesBioconjugationPhysiological Probes

Posted on May 12, 2022
Do atoms gain or lose mass when they react with each other?
Chemical ReagentsBioconjugationCell Metabolism

Posted on May 12, 2022
Why do some chemical bonds need more energy to break?
Chemical ReagentsClassic DyesCrosslinkersBioconjugation

Posted on May 9, 2022
What are the building blocks of nucleic acids?
NucleusDNA and RNA QuantitationPhysiological ProbesBioconjugation

Posted on May 9, 2022
What is the Difference Between Golgi Apparatus and Endoplasmic Reticulum?
Golgi ApparatusEndoplasmic Reticulum (ER)Cell Structures and OrganellesBioconjugation

Posted on May 6, 2022
What type of transport moves material against the concentration gradient with the use of energy?
Physiological ProbesBioconjugationCell ViabilityCellular ProcessesEnzymes

Posted on May 6, 2022
What route is used to export proteins from the cell?
Endoplasmic Reticulum (ER)Cell Structures and OrganellesBioconjugationGolgi ApparatusPlasma Membrane

Posted on April 28, 2022
What are the primary types of cells?
Cell Structures and OrganellesCell ViabilityPhysiological ProbesBioconjugationCell Viability

Posted on April 28, 2022
What are the differences between HeLa cells and cancer cells?
BioconjugationPhysiological ProbesCell Metabolism

Posted on April 27, 2022
What are the similarities between HeLa cells and cancer cells?
BioconjugationPhysiological Probes

Posted on April 27, 2022
What lipids are found in the plasma membrane?
BioconjugationCell Structures and Organelles

Posted on April 15, 2022
What are the differences between dilution and dilution factor?
BioconjugationTools

Posted on April 15, 2022
Is the cell membrane the same as the plasma membrane?
Cell Structures and OrganellesBioconjugation

Posted on April 14, 2022
What are the differences between lysine and arginine?
Cell Structures and OrganellesChemical ReagentsPhysiological ProbesBioconjugation

Posted on April 14, 2022
What are the categories of amino acids?
Cell Structures and OrganellesChemical ReagentsBioconjugationPhysiological ProbesEnzymes

Posted on March 25, 2022
What are the applications of capillary electrophoresis?
BioconjugationChemical ReagentsDyes by Functional GroupAntibody and Protein Labeling

Posted on March 25, 2022
How can I improve PCR efficiency?
BioconjugationChemical ReagentsDNA and RNA Quantitation

Posted on March 24, 2022
How do I determine the amplification efficiency of my qPCR assay?
Chemical ReagentsDNA and RNA QuantitationAntibody and Protein LabelingBioconjugation

Posted on March 24, 2022
What is RT-qPCR (Quantitative Reverse Transcription PCR)?
DNA and RNA QuantitationChemical ReagentsBioconjugationPhysiological Probes

Posted on March 22, 2022
What factors affect the specificity of PCR?
BioconjugationPhysiological ProbesPolymerase Chain Reaction (PCR)DNA and RNA Quantitation

Posted on March 22, 2022
What are the steps of reverse transcription?
BioconjugationNucleusDNA and RNA QuantitationPhysiological Probes

Posted on March 22, 2022
Which viruses use reverse transcriptase?
NucleusDNA and RNA QuantitationBioconjugationPhysiological Probes

Posted on March 22, 2022
What is a common reverse transcriptase?
BioconjugationNucleusDNA and RNA Quantitation

Posted on March 18, 2022
What are the common affinity tags for protein purification?
BioconjugationPhysiological ProbesAntibody and Protein LabelingAntibodies and Proteomics

Posted on March 18, 2022
What is the role of NaCl in lysis buffer?
BioconjugationPhysiological ProbesCell Culture

Posted on March 18, 2022
What is EDTA used for in chemistry?
Physiological ProbesBioconjugationAntibodies and Proteomics

Posted on March 18, 2022
What is Column Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on March 18, 2022
What is Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on March 18, 2022
What are the main phases of Column Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation

Posted on March 18, 2022
What are the commonly used chromatographic methods for protein purification?
BioconjugationPhysiological ProbesAntibody and Protein LabelingAntibodies and Proteomics

Posted on March 16, 2022
What is an elution buffer and why is it necessary?
BioconjugationToolsPhysiological Probes

Posted on March 11, 2022
Why is EDTA used in protein purification?
BioconjugationPhysiological ProbesAntibodies and Proteomics

Posted on March 11, 2022
Which chromatography is best for protein purification?
BioconjugationAntibodies and ProteomicsAntibody and Protein Labeling

Posted on March 11, 2022
How does column chromatography work?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation

Posted on March 11, 2022
How does affinity purification work?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation

Posted on March 4, 2022
What is affinity purification?
BioconjugationBiotin and Streptavidin

Posted on February 18, 2022
What are the differences between next-generation sequencing (NGS) and qPCR?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationBioconjugation

Posted on February 17, 2022
What is the purpose of DNA fragmentation?
BioconjugationCellular ProcessesCell Viability Assays

Posted on February 16, 2022
What are the main advantages of NGS over classical Sanger sequencing?
Coronavirus (SARS-CoV-2)BioconjugationDNA and RNA QuantitationNucleusEnzymes

Posted on February 10, 2022
What are the commonly used discontinuous gel buffer systems?
Gel ElectrophoresisPhysiological ProbesBioconjugationBuffer Preparations and Recipes

Posted on February 9, 2022
What are the differences between Sanger sequencing and NGS?
NucleusDNA and RNA QuantitationPhysiological ProbesBioconjugation

Posted on February 9, 2022
What is the difference between Immunoblot and Western Blot?
BioconjugationPhysiological ProbesBiotin and Streptavidin

Posted on February 4, 2022
What are the applications of Immunoblot?
Immunohistochemistry (IHC)BioconjugationPhysiological Probes

Posted on February 1, 2022
What are the different Next Generation Sequencing (NGS) technologies?
Physiological ProbesBioconjugationDNA and RNA Quantitaion

Posted on February 1, 2022
What are the differences between organoids and spheroids?
Physiological probesBioconjugation

Posted on February 1, 2022
What are the differences between organ cultures and cell cultures?
Membrane Potential and ChannelsPhysiological ProbesIntracellular IonsBioconjugation

Posted on January 28, 2022
What is a neurosphere formation?
Cell CytotoxicityCellular ProcessesPhysiological ProbesBioconjugation

Posted on January 28, 2022
What are the differences between 2D and 3D cell cultures?
Cell Cycle AssaysPhysiological ProbesBioconjugationCellular Processes

Posted on January 27, 2022
What are the differences between cell membrane and cell wall?
BioconjugationCell Structures and Organelles

Posted on January 27, 2022
What are the differences between active and passive membrane transport?
BioconjugationCell Structures and OrganellesPlasma Membrane

Posted on January 26, 2022
What kinds of passive transport are possible through the plasma membrane?
Plasma MembraneBioconjugationCell Structures and Organelles

Posted on January 26, 2022
What is the fluid mosaic model?
Plasma MembraneBioconjugationCell Structures and OrganellesPhysiological Probes

Posted on January 26, 2022
What are the two layers of the plasma membrane?
BioconjugationCell Structures and OrganellesPlasma Membrane

Posted on January 25, 2022
What types of membrane proteins are there?
Membrane Potential and ChannelsCell Structures and OrganellesBioconjugation

Posted on January 25, 2022
What is the lipid bilayer?
Cell Structures and OrganellesBioconjugation

Posted on January 20, 2022
What is the plasma membrane mostly made of?
BioconjugationCell Structures and OrganellesPlasma Membrane

Posted on January 20, 2022
What labels are used for the plasma membrane?
Plasma MembraneBioconjugation

Posted on January 20, 2022
How is the plasma membrane visualized?
BioconjugationCell Structures and Organelles

Posted on January 6, 2022
What is a tumoroid?
Physiological ProbesBioconjugation

Posted on January 6, 2022
What do the peaks in mass spectrometry represent?
Antibodies and ProteomicsPhysiological ProbesBioconjugation

Posted on December 23, 2021
What are the differences between ion exchange chromatography and affinity chromatography?
Antibodies and ProteomicsBioconjugationAntibody and Protein LabelingProtein to Protein Conjugation

Posted on December 23, 2021
What is the difference between 7-AAD and Propidium Iodide?
DNA and RNA QuantitationNucleusBioconjugationPhysiological Probes

Posted on December 21, 2021
What’s the difference between organ cultures and cell cultures?
BioconjugationPhysiological Probes

Posted on December 17, 2021
What are the different types of chromatography?
BioconjugationPhysiological ProbesDNA and RNA Quantitation

Posted on December 16, 2021
What is the difference between an isocratic and a gradient elution method?
Antibodies and ProteomicsBioconjugationPhysiological Probes

Posted on December 15, 2021
What elutes first in affinity chromatography?
BioconjugationPhysiological ProbesAntibodies and Proteomics

Posted on December 14, 2021
What is protein purification used for?
Physiological ProbesBioconjugationAntibody and Protein Labeling

Posted on December 14, 2021
What are the applications of immobilized metal affinity chromatography (IMAC)?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics

Posted on December 14, 2021
How do you elute the target protein in affinity chromatography?
Physiological ProbesBioconjugationBiotin and Streptavidin

Posted on November 15, 2021
What instruments are necessary to perform spectral flow cytometry?
Spectral Flow CytometryBioconjugationFlow Cytometry Reagents

Posted on November 15, 2021
What is spectral unmixing?
BioconjugationFlow Cytometry Reagents

Posted on November 11, 2021
Does spectral flow cytometry require signal subtraction?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
Who invented spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
What is the difference between conventional and spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
What is full spectrum flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on October 29, 2021
What’s the difference between FITC and FAM?
FluoresceinsPeptide and Oligonucleotide LabelingClassic DyesBioconjugation

Posted on October 20, 2021
What are the 3 top guidelines for using PerCP conjugates and tandems?
PE and APCBioconjugation

Posted on September 30, 2021
Is rhodamine a fluorochrome?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional GroupsBioconjugation

Posted on September 24, 2021
What is pre-adsorption?
Secondary AntibodiesPE and APCBioconjugationProtein to Protein Conjugation

Posted on September 24, 2021
What steps can be taken to avoid degradation of tandem dyes?
PE and APCBioconjugation

Posted on September 24, 2021
What is the major limitation of tandem dyes?
PE and APCBioconjugation

Posted on September 23, 2021
Are tandem dyes sensitive to fixatives and permeabilization agents?
PE and APCBioconjugation

Posted on September 23, 2021
Why are tandem dyes not recommended for intracellular staining?
BioconjugationPE and APC

Posted on September 23, 2021
What are tandem dyes? What is the advantage of using tandem dyes?
BioconjugationProtein to Protein ConjugationPE and APC

Posted on September 22, 2021
What are the common applications for secondary antibody conjugates?
Secondary AntibodiesBioconjugationImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling

Posted on September 21, 2021
What does HRP bind to?
BioconjugationAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPProtein to Protein Conjugation

Posted on September 13, 2021
What increases membrane fluidity?
BioconjugationPlasma Membrane

Posted on September 13, 2021
What are the main functions of the plasma membrane?
BioconjugationPlasma MembraneCell Structures and Organelles

Posted on September 13, 2021
What are the main proteins in the cell membrane?
BioconjugationCell Structures and Organelles

Posted on September 13, 2021
How does the cell membrane work?
Amplite® Reagents and Assay KitsBioconjugationCell Cytotoxicity

Posted on September 13, 2021
Does the plasma membrane contain cholesterol?
BioconjugationPlasma Membrane

Posted on September 13, 2021
Where is the cell membrane located?
BioconjugationCell Structure and Organelles

Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) the only thymidine analog?
Cell Proliferation AssaysBioconjugationCell CytotoxicityCell Viability

Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) staining performed in vivo?
Cell Proliferation AssaysBioconjugationCell ViabilityCell Cytotoxicity

Posted on September 10, 2021
Can BrdU (Bromodeoxyuridine) be used in vitro?
BioconjugationCell ViabilityCell CytotoxicityApoptosis and Necrosis

Posted on September 10, 2021
How does BrdU (Bromodeoxyuridine) cause mutation?
Cell ViabilityBioconjugationCell Cytotoxicity

Posted on September 9, 2021
What are terminal transferases?
BioconjugationCell ViabilityTUNEL

Posted on August 25, 2021
What is the unfolded protein response?
Endoplasmic Reticulum (ER)Cell Structures and OrganellesBioconjugation

Posted on August 18, 2021
What does methylene blue indicate?
BioconjugationHorseradish Peroxidase (HRP) and Poly-HRPEnzymes

Posted on August 18, 2021
Is methylene blue toxic?
EnzymesBioconjugation

Posted on August 18, 2021
What does Giemsa bind to?
Physiological ProbesDNA and RNA QuantitationBioconjugationNucleus

Posted on August 18, 2021
What is Giemsa stain?
NucleusDNA and RNA QuantitationCell Structures and OrganellesBioconjugation

Posted on August 13, 2021
What is the structure of (ICG)?
BioconjugationChemical ReagentsAntibody and Protein Labeling

Posted on August 13, 2021
Can indocyanine green (ICG) be conjugated to antibodies?
BioconjugationChemical ReagentsAntibody and Protein Labeling

Posted on August 12, 2021
Can indocyanine green (ICG) be used in Western blotting?
BioconjugationChemical Reagents

Posted on August 12, 2021
Is indocyanine green (ICG) used for small animal in vivo imaging?
BioconjugationChemical ReagentsClassic Dyes

Posted on August 12, 2021
What are indocyanine green (ICG) hydrazides used for?
BioconjugationChemical Reagents

Posted on August 12, 2021
Are there any alternatives to indocyanine green (ICG)?
Chemical ReagentsBioconjugation

Posted on August 10, 2021
What are indocyanine green (ICG) injections used for?
BioconjugationChemical ReagentsDyes by Functional Group

Posted on August 10, 2021
How is indocyanine green administered?
BioconjugationChemical Reagents

Posted on July 30, 2021
What are the most common ionic detergents?
BioconjugationCell Structures and Organelles

Posted on July 28, 2021
How do ionic detergents affect protein molecules?
Plasma MembraneBioconjugation

Posted on July 28, 2021
What is the difference between ionic and nonionic detergents?
Physiological ProbesBioconjugationPlasma Membrane

Posted on July 28, 2021
What do ionic detergents do?
BioconjugationCell Structure and Organelles

Posted on July 28, 2021
What is a common feature between ionic and nonionic detergents?
BioconjugationPlasma Membrane

Posted on July 27, 2021
Are there any alternatives to Cy5?
CyaninesBioconjugationiFluor Dyes and Kits

Posted on July 20, 2021
What kinds of biomolecules can cyanine dyes label?
BioconjugationAntibody and Protein Labeling

Posted on July 1, 2021
What color does Cy5 fluoresce?
BioconjugationPhysiological Probes

Posted on July 1, 2021
What color is Cy3?
BioconjugationClassic DyesCynines

Posted on July 1, 2021
Is PE Cy7 photosensitive?
PE and APCClassic DyesBioconjugation

Posted on July 1, 2021
Is Cy3 a fluorophore?
CyaninesClassic DyesBioconjugation

Posted on July 1, 2021
Is Cy5 hydrophobic?
CyaninesClassic DyesBioconjugation

Posted on June 17, 2021
What kind of targets can a lateral flow immunoassay (LFIA) detect?
EnzymesBioconjugation

Posted on June 8, 2021
Do cytokines cause pain?
BioconjugationPhysiological Probes

Posted on June 8, 2021
How are cytokine levels tested?
BioconjugationCell SignalingEnzyme-Linked Immunosorbent Assay (ELISA)

Posted on June 3, 2021
What is the meaning of immunoassay?
BioconjugationEnzyme-Linked Immunosorbent Assay (ELISA)Enzymes

Posted on June 3, 2021
How are lateral flow immunoassays (LFIAs) developed?
Bioconjugation

Posted on June 3, 2021
How does Immunoassay Test Work?
EnzymesBioconjugation

Posted on May 27, 2021
How many cells are required for immunoprecipitation?
Antibody and Protein LabelingBioconjugation

Posted on May 27, 2021
What is the purpose of chromatin immunoprecipitation?
Protein to Protein ConjugationBioconjugation

Posted on May 27, 2021
How do you increase immunoprecipitation efficiency?
BioconjugationAntibody and Protein Labeling

Posted on May 26, 2021
Is immunoprecipitation quantitative?
Antibody and Protein LabelingBioconjugation

Posted on May 26, 2021
How much protein is required for immunoprecipitation?
Antibody and Protein LabelingBioconjugation

Posted on May 26, 2021
What’s the difference between direct immunoprecipitation and indirect immunoprecipitation (IP)?
Antibody and Protein LabelingBioconjugation

Posted on May 26, 2021
What is the difference between immunoprecipitation and coimmunoprecipitation?
BioconjugationAntibodies and ProteomicsImmunohistochemistry (IHC)

Posted on May 25, 2021
What are the applications of immunoprecipitation?
Protein to Protein ConjugationBioconjugation

Posted on May 25, 2021
What is immunoprecipitation?
BioconjugationProtein to Protein Conjugation

Posted on May 20, 2021
What is input in immunoprecipitation?
Bioconjugation

Posted on May 19, 2021
Can luciferin be used as a substrate?
EnzymesLuciferasesBioconjugation

Posted on May 18, 2021
What is reciprocal immunoprecipitation?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation

Posted on May 12, 2021
What controls the rate at which luciferin breaks down?
LuciferasesEnzymesBioconjugation

Posted on May 7, 2021
What is a biotinylated secondary antibody?
BioconjugationSecondary Antibodies

Posted on May 7, 2021
What is biotin labeling?
BioconjugationAntibody and Protein Labeling

Posted on May 7, 2021
What is the difference between conjugated and unconjugated antibodies?
BioconjugationEnzyme-Linked Immunosorbent Assay (ELISA)

Posted on May 6, 2021
What kind of excitation light does Hoechst require?
NucleusDNA and RNABioconjugation

Posted on May 5, 2021
Is Hoechst cytotoxic?
NucleusDNA and RNABioconjugation

Posted on May 5, 2021
What does Hoechst stain?
NucleusDNA and RNABioconjugation

Posted on April 30, 2021
How is Forster distance calculated?
Bioconjugation

Posted on April 23, 2021
What are the essential components of the cell membrane?
Cell Structures and OrganellesBioconjugation

Posted on April 23, 2021
How are membranes classified according to their permeability?
Cell Structures and OrganellesBioconjugation

Posted on April 20, 2021
Which buffers are recommended for immobilizing biotinylated molecules?
BioconjugationAntibody and Protein Labeling

Posted on April 20, 2021
How many biotin binding-sites are there per streptavidin molecule?
BioconjugationBiotin and Streptavidin

Posted on March 17, 2021
What kind of detector is used in fluorimetry?
BioconjugationChemical ReagentsiFiFluor Dyes and Kits

Posted on March 17, 2021
What does distance have to do with FRET efficiency?
Peptide and Oligonucleotide LabelingFörster Resonance Energy Transfer (FRET)Bioconjugation

Posted on February 11, 2021
What is the difference between G1 and S phase in the cell cycle?
DNA and RNABioconjugationPhysiological ProbesNucleus

Posted on February 11, 2021
How much BSA should I add to PCR?
DNA and RNAPhysiological ProbesBioconjugation

Posted on February 11, 2021
What is the function of BSA in PCR?
BioconjugationAntibody and Protein Labeling

Posted on February 4, 2021
Why does DNA not form protein directly?
Physiological ProbesDNA and RNABioconjugation

Posted on February 3, 2021
What are the differences between prokaryotes and eukaryotes?
BioconjugationDNA and RNA

Posted on January 28, 2021
What are the results of exocytosis?
BioconjugationPlasma MembraneCell Signaling

Posted on January 26, 2021
What is the pH of DNA?
DNA and RNANucleusPhysiologicalBioconjugation

Posted on January 21, 2021
What is the function of endosomes?
BioconjugationCell Structure and Organelles

Posted on January 21, 2021
How does exocytosis affect the membrane?
BioconjugationCell Structures and Orgaelles

Posted on January 8, 2021
What is the relationship between DNA, RNA and proteins?
NucleusDNA and RNABioconjugationPhysiological Probes

Posted on January 8, 2021
Why are plasmids used in recombinant DNA technology?
BioconjugationCellular Processes

Posted on January 8, 2021
Can a virus get infected by a virus?
EnzymesBioconjugation

Posted on December 1, 2020
What are the reasons for high background in ELISA?
ELISAAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)BioconjugationHorseradish Peroxidase (HRP) and Poly-HRP

Posted on November 25, 2020
What are the common methods for nucleic acid labeling?
Physiological ProbesNucleusDNA and RNABioconjugation

Posted on November 9, 2020
How do I store my antibodies?
BioconjugationProtein to Protein Conjugation