AAT Bioquest


Posted on March 13, 2020
What is an Indirect ELISA?
ELISAprimary antibodysecondary antibodyCell AssaySignal Amplificationmicroplate reader

Posted on February 19, 2020
What does TUNEL mean? What is a TUNEL assay?
apoptosisTUNELCell AssayCell Cycle Analysiscell death

Posted on February 19, 2020
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer

Posted on February 19, 2020
My cells are dying after transfection-what solutions do you recommend?
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death

Posted on January 2, 2020
Why does my sample with the most dense cells have the least amount of fluorescence for resazurin?
Cell CytotoxicityCell Assaycell deathcell toxicity

Posted on December 11, 2019
Can I Gram stain living cells?
Cell AssayCell CytotoxicityFixationGram stainingbacteriafluorescence imaginglive cell assay

Posted on December 10, 2019
Why do cells recovered from liquid nitrogen have lower viability?
cell cultureCell Assaylive cell analysis

Posted on December 10, 2019
What are the differences between traditional vs modern Gram staining?
Cell AssayColorimetricGram stainingfluorimetriclive cell assaybacteria

Posted on December 6, 2019
How do you activate T cells?
Cell AssayELISAIHCT cellantibody labelingmicroplate

Posted on October 22, 2019
What are the differences between MTT and WST assays?
AbsorbanceCell ViabilityCell AssayCell CytotoxicityColorimetricWST-8cell proliferation

Posted on September 27, 2019
What is an ADME-Tox assay?
Cell Assaylive cell assay

Posted on August 26, 2019
Why are cells lysed?
Cell AssayCell ViabilityWestern Blotcell lysishomogenouscentrifugeIHCSignal Amplificationantibody labelingimmunohistochemistry

Posted on June 13, 2019
How to do compensation in flow cytometer with JC-10 stained cells?
Cell AssayJC-10compensation correctionlive cell analysislive cell assay

Posted on June 13, 2019
How do you detach cells for flow cytometric analysis when using Cell Meter™ Fluorimetric Intracellular ROS Activity Assay?
ROSCell Assayflow cytometer

Posted on June 12, 2019
Can I customize ELISA techniques for my experiment?
cell assayhorseradish peroxidaseCell Assayhumanlive cell assaymicroplatemicroplate readerready-to-useCustomizationELISA

Posted on June 12, 2019
Are there different kinds of ELISA?
Cell AssayConjugationfluorescent plate readermicroplate readerELISADirect ELISA

Posted on April 2, 2019
What does my lab need to run ELISA?
Cell AssayELISADirect ELISAabsorbanceflow cytometerfluorescence microplate readermicroplate readerprimary antibody

Posted on April 2, 2019
How long does it take to get results from ELISA?
Cell AssayDirect ELISAELISA

Posted on April 2, 2019
Does EDTA interfere with your Cell Meter™ Colorimetric Antioxidant Activity Assay Kit (Cat # 15900)?
AbsorbanceH2O2HRPABTSCell Assay

Posted on April 2, 2019
Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
Actin iFluor dyesCell AssayFixationF-actinFixativesfluorescence imagingfluorescence microscopephalloidin

Posted on April 2, 2019
Can Live or Dead™ Fixable Dead Cell Staining Kit (Cat# 22601) be used to stain cells after fixation?
Cell AssayOrganelle Stainingfluorimetric

Posted on April 2, 2019
What is the size range of mammalian cells?
Cell Assay

Posted on April 2, 2019
What is a Competition ELISA?
ELISAprimary antibodysecondary antibodymicroplatemicroplate readerenzyme-labeledcell assayCell AssayCalculating Concentrationwavelength

Posted on July 8, 2018
What are the differences between your fluorimetric peroxynitrite assays (Cat# 16315, 16316 and 16317)?
ROSCell Assayflow cytometerperoxynitrite anionfluorescence imagingfluorescent microscope

Posted on February 26, 2018
How can I lyse my cells without lysing the nuclear membrane?
cell lysisCell Assay

Posted on January 18, 2018
Before staining actin with phalloidin dye conjugates, how should I fixate my sample?
fluorimetricOrganelle StainingActinCell AssayPhalloidinFixatives