logo
AAT Bioquest

FAQ

Posted on March 9, 2023
What are the most commonly used biological stains?
Classic DyesDAPIPhysiological Probesethidium bromide


Posted on January 26, 2023
What are the commonly used non-fixed cell viability dyes?
Cell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesDAPINucleusPropidium iodide (PI)


Posted on December 10, 2022
Does DAPI stain all cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 10, 2022
Can DAPI bind to RNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 10, 2022
Do I have to fix cells before DAPI staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
Is DAPI toxic to cells?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What is DAPI a marker of?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What organelle does DAPI stain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
Why is DAPI used as a counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What should I consider when choosing counterstain?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular ProcesssesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What procedures may be required to prepare a sample for cellular staining?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on December 2, 2022
What are the common cell stains?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)


Posted on December 2, 2022
What laser excites DAPI?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCellular ProcessesCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Nucleus


Posted on July 22, 2020
How does DAPI work?
Antibodies and Proteomics Bioconjugation Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesDAPI


Posted on May 18, 2020
How do DNA binding dyes bind to DNA?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingCell Cycle AssaysCellular Structures and OrganellesDAPIImmunohistochemistry (IHC)Physiological ProbesPropidium iodide (PI)TUNEL


Posted on May 9, 2020
Is it possible to use DAPI and PI simultaneously?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDAPIFluorescenceImmunohistochemistry (IHC)Propidium iodide (PI)


Posted on April 24, 2020
What is the right DAPI concentration to use in a flow cytometry experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on July 24, 2018
What is the difference between DAPI and Hoechst?
DNADAPILive or DeadHoechst Dyes