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AAT Bioquest

FAQ

Posted on March 20, 2023
What are the similarities between multicellular and unicellular?
Cellular Structures and OrganellesDNANucleusPlasma Membrane


Posted on February 22, 2023
How can I tell whether a regulator protein is an activator or an inhibitor?
Cellular Structures and OrganellesDNADNA and RNANucleic AcidsNucleus


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 11, 2023
What are the differences between genes and chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What is the relationship between genes and chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What do chromosomes look like?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the differences between DNA and chromosome?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the major types of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the functions of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the differences between the prokaryotic and eukaryotic chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the similarities between the prokaryotic and eukaryotic chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationPhysiological Probes


Posted on January 11, 2023
What are the main components of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 6, 2023
What are the differences between eukaryotic DNA and prokaryotic DNA?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCellular Structures and OrganellesDNAImmunohistochemistry (IHC)Nucleic AcidsNucleus


Posted on September 16, 2022
Where does DNA replication occur?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesDNA


Posted on August 25, 2022
Do chloroplasts have DNA?
Cellular ProcessesCellular Structures and OrganellesDNAOrganellesplant cells


Posted on August 3, 2021
Do you have any dyes that can stain the nuclei in live cells? Are they also fixable and can I use them to stain fixed cells?
Nuclei StainingDNAFluorescenceemission wavelengthfixablefixed cellsfluorescence imaginglive cell analysisnuclear DNAMicroscopy


Posted on November 9, 2020
What are the steps of DNA extraction?
DNADNA extractionGenomic DNAProtocol


Posted on October 19, 2020
Why does my DNA oligonucleotide have mutations?
Peptide and Oligonucleotide LabelingDNADNA and RNAPeptide and Oligonucleotide


Posted on October 14, 2020
What are the differences between transcription and replication?
Cell Structures and OrganellesDNARNA


Posted on October 14, 2020
What is Gibson Assembly used for?
DNARNA


Posted on October 13, 2020
What are the differences between transcription and translation?
Cell Structures and OrganellesDNARNA


Posted on October 13, 2020
What is transcription in biology?
DNARNA


Posted on September 30, 2020
What is the best DNA extraction method for genomics applications?
DNADNA and RNA QuantitationDNA and RNADNA extractionPolymerase Chain Reaction (PCR)


Posted on September 30, 2020
How do I extract DNA from bacteria?
DNADNA extraction


Posted on September 28, 2020
How do I isolate plasmid DNA from E coli?
DNADNA and RNAPhysiological Probes


Posted on September 25, 2020
How do I remove genomic DNA contamination from isolated plasmid DNA?
DNADNA and RNA QuantitationDNA PurityDNA and RNA


Posted on September 25, 2020
How do DdNTPs stop a sequencing reaction?
DNA


Posted on September 18, 2020
What is the difference between plasmid DNA and chromosomal DNA?
DNAintracellular pH


Posted on September 16, 2020
What is gene sequencing?
DNAdna sequencinggene sequencing


Posted on September 2, 2020
How do I purify a contaminated DNA sample?
DNARNANucleusPhysiological Probes


Posted on September 1, 2020
What is DdNTP?
DdNTPddTTPDNADNA sequencing


Posted on July 22, 2020
What methods are used in next-generation sequencing (NGS) to amplify templates?
DNADNA and RNADNA sequencingPolymerase Chain Reaction (PCR)gene sequencing


Posted on July 22, 2020
How does bridge amplification work in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by synthesis approach in next-generation sequencing (NGS)?
Cellular processesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by ligation approach in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is a reversible terminator used in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What types of reversible terminators are available for next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the major platforms for next generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is the general procedure of Illumina sequencing?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is pyrosequencing?
DNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does pyrosequencing work?
DNADNA and RNA QuantitationDNA and RNADNA sequencingEnzymesgene sequencing


Posted on July 22, 2020
What is third-generation sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does propidium iodide staining work?
Cell Cycle AnalysisCell Cycle AssaysCell ViabilityCell Viability AssaysCellular ProcessesDNAPropidium iodide (PI)


Posted on July 22, 2020
How do you design sgRNA sequences for CRISPR-Cas9?
DNADNA and RNAEnzymesRNAbacteriadna sequencing


Posted on July 22, 2020
What is difference between CRISPR interference and CRISPR activation?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on July 22, 2020
What is the difference between CRISPRi and RNAi?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on July 22, 2020
How does siRNA work in RNA interference (RNAi)?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on July 22, 2020
What are the differences between miRNA and siRNA?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What are the different kinds of RNAs and what do they do?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is gene silencing?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is the difference between gene silencing and gene knockout?
DNADNA and RNANucleic AcidsRNAgene sequencing


Posted on July 22, 2020
How do antisense oligonucleotides work in gene silencing?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideRNAgene sequencing


Posted on July 22, 2020
What are the types of DNA repair mechanisms?
DNADNA and RNANucleic AcidsRNAdsDNAssDNA


Posted on July 22, 2020
What is the difference between DNA damage and DNA mutation?
DNADNA and RNADNA sequencingRNAdsDNAssDNA


Posted on July 22, 2020
What is the role of p53 in DNA damage repair system?
Cell Cycle AnalysisDNADNA and RNAProteinRNA


Posted on July 22, 2020
What is fluorescence in situ hybridization (FISH)?
DNADNA and RNAFISHFluorescenceNucleic AcidsRNAfluorescence microscopein situ hybridization


Posted on July 22, 2020
What are the general steps of fluorescence in situ hybridization (FISH) experiments?
DNADNA and RNAFISHFluorescencePolymerase Chain Reaction (PCR)RNAin situ hybridizationssDNA


Posted on July 22, 2020
What is nick translation?
DNADNA and RNADNA sequencingFISHPeptide and Oligonucleotidein situ hybridization


Posted on June 22, 2020
What is the protospacer adjacent motif (PAM) in CRISPR-Cas9?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How is Cas prevented from cutting its own CRISPR locus?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How does CRISPR-Cas9 edit genes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What are the two main types of DNA repair mechanisms employed in CRISPR-Cas9?
DNADNA and RNAEnzymesbacteriadna sequencing


Posted on June 22, 2020
How is CRISPR-Cas9 used in gene knockout and knock-in?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is dCas9? And what is its application?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What are introns?
DNADNA and RNADNA sequencingNucleic AcidsProteinRNAgene sequencing


Posted on June 22, 2020
What are the types of introns?
Antibodies and ProteomicsDNADNA and RNAProteinRNA


Posted on June 22, 2020
What are the functions of introns?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the three major steps in mRNA processing?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is the function of the 5’ cap and 3’ poly-A tail in mRNA?
DNADNA and RNAPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What are exons?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNA


Posted on June 22, 2020
What is needed for translation?
Cellular ProcessesDNADNA and RNAProteinRNA


Posted on June 22, 2020
What is the function of ribosome?
Cellular ProcessesDNADNA and RNANucleic AcidsProteinRNA


Posted on June 22, 2020
What is a codon?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What are the three stages of translation?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What are the 3 binding sites for tRNA in the ribosome?
Cellular processesDNADNA and RNANucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on June 22, 2020
What happens to mRNA after it gets translated?
Cellular ProcessesDNADNA and RNANucleic AcidsProteinRNA


Posted on June 22, 2020
What is exon shuffling?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on June 22, 2020
What is genetic recombination?
Cellular ProcessesDNADNA and RNANucleic AcidsRNA


Posted on June 22, 2020
What is recombinant DNA?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is molecular cloning?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between PCR and molecular cloning regarding DNA replication?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is a cloning vector in molecular biology?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the essential features of a plasmid cloning vector?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
Why are plasmids used as vectors in genetic engineering?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is transformation in molecular biology?
Cellular ProcessesDNADNA and RNANucleic AcidsTransfectionTransformation


Posted on June 22, 2020
What is horizontal gene transfer?
Cellular ProcessesConjugationDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Transformation


Posted on June 22, 2020
What is cloned DNA used for?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What is the difference between cloning and expression vectors?
Cellular ProcessesDNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What is CRISPR?
DNADNA and RNADNA sequencingNucleic Acidsbacteria


Posted on June 22, 2020
What is the CRISPR-Cas system in prokaryotes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What are the three stages in the CRISPR-Cas defense system?
DNADNA and RNAEnzymesbacteriadna sequencing


Posted on June 22, 2020
What is CRISPR-Cas9 technology?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is a single guide RNA?
DNADNA and RNADNA sequencingEnzymesRNA


Posted on June 22, 2020
What are the main components of CRISPR-Cas9 technique?
DNADNA and RNAEnzymesRNAbacteria


Posted on June 22, 2020
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
What is a sense strand (or coding strand)?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)dsDNAssDNA


Posted on June 22, 2020
Why should primers used in PCR have similar melting temperatures?
DNAPCRPCR primerPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What is the difference between melting temperature and annealing temperature?
DNAPCRPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What are the advantages of Taq polymerase in PCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of Taq polymerase?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of polymerases used in PCR?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What are the three stages of a PRC process?
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of PCR technology?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How does SYBR Green work in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
How do hydrolysis probes work in qPCR? probes work?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What is the melting curve analysis in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on May 18, 2020
Why is RNA extraction tougher than DNA extraction?
Cellular Structures and OrganellesDNADNA and RNADNA extractionExtractionRNA


Posted on May 9, 2020
What is the function of MgCl2 in PCR?
Chemical ReagentsDNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Polymerasesmagnesium


Posted on May 9, 2020
What is the best way to store a buffy coat?
Antibodies and ProteomicsDNADNA extractionIHCImmunohistochemistry (IHC)Storage


Posted on April 24, 2020
What is the difference between 'operon' and 'transcription unit'?
Cellular ProcessesCellular Structures and OrganellesDNADNA and RNAEnzymesNucleic AcidsPeptide and OligonucleotideProteinRNA


Posted on April 24, 2020
What is the difference between agroinfiltration and agroinfection?
Antibodies and ProteomicsCell CultureCellular Structures and OrganellesDNAplant cells


Posted on February 19, 2020
How do I improve transfection efficiency?
ControlsDNA Concentration CalculatorDNAStorageTransfectioncell culturedegree of labelinglipid


Posted on February 19, 2020
My cells are dying after transfection-what solutions do you recommend?
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death


Posted on September 12, 2019
What is the difference between agarose and polyacrylamide gels?
DNAProteinagarose gelpolyacrylamide gelIgGelectrophoresis


Posted on February 27, 2019
What is the extinction coefficient of DRAQ5?
extinction coefficientDRAQ5DNA


Posted on July 24, 2018
What is the difference between DAPI and Hoechst?
DNADAPILive or DeadHoechst Dyes


Posted on July 8, 2018
Is your nucleic acid stain, Nuclear Red LCS1 (Cat# 17542), fixable?
DNAfixableNuclei Staining


Posted on July 8, 2018
Other than Ethidium Bromide (EBr), what are some alternative options for staining nucleic acids in agarose gels?
EBrDNASYBR Goldagarose gelnucleic acidSYBR Greenethidium bromide


Posted on March 8, 2018
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
DNARNAdual stainsnucleic acidfluorescence imaging