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AAT Bioquest

Posted on November 18, 2023
Can RNA and DNA ladders be used interchangeably?
DNA and RNA QuantitationGel ElectrophoresisPCR Detection of Viral DNA/RNADNA & RNA Markers and Ladders


Posted on October 10, 2023
What causes cells to stop growing?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProlferationCell Cycle and ProliferationCell SenescenceDNA and RNA Quantitation


Posted on September 18, 2023
What are the differences between cell biology and molecular biology?
BioconjugationCellular Structures and OrganellesDNA and RNA QuantitationPhysiological Probes


Posted on September 18, 2023
Do microbes have DNA?
BioconjugationCellular Structures and OrganellesDNA and RNA QuantitationMycoLight Dyes and Kitsbacteria


Posted on September 18, 2023
What are the similarities between biology and microbiology?
Cellular Structures and OrganellesDNA and RNA QuantitationPhysiological Probesbacteria


Posted on September 18, 2023
What are the differences between biology and microbiology?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationMycoLight Dyes and KitsPhysiological Probesbacteria


Posted on August 29, 2023
What are molecular beacons used for?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the best ways to store a molecular beacon preparation?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
What are the reasons for the low signal-to-background ratio after the synthesis and purification of a molecular beacon and how do I solve the problem?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)PurificationReal-Time PCR (qPCR)


Posted on August 23, 2023
Is gel electrophoresis a type of chromatography?
Chromatographic techniquesDNA and RNA QuantitationGel Electrophoresis


Posted on August 23, 2023
What are the differences between chromatography and electrophoresis?
Chromatographic techniquesDNA and RNA QuantitationGel Electrophoresis


Posted on August 16, 2023
What are the requirements of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the steps involved in DNA based microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the principles of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the applications of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the differences between DNA microarray and RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the pros of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the cons of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the cons of RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the pros of RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the differences between karyotype and chromosome microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the similarities between karyotype and chromosome microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the types of microarrays based on the mode of preparation?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the types of microarrays based on the probes used?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the limitations of karyotyping?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What can CMAs not detect?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the different types of chromosome studies?
DNA and RNA QuantitationFISHNucleic AcidsNucleusgene sequencingin situ hybridization


Posted on August 16, 2023
What are the differences between microarray and next generation sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the similarities between microarray and next generation sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the challenges of chromosomal microarrays?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the benefits of SNPs?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What types of disorders can chromosome microarray analysis diagnose?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What is the general workflow of SNP microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 14, 2023
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the applications of PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq polymerase do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do I do if my PCR isn't working?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq buffer do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do forward and reverse primers do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the similarities between forward and reverse primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the steps involved in getting the reverse primer sequence?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the guidelines should I follow when designing primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why is it necessary to purify PCR products?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why are quenchers used in real time PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use one-step as opposed to two-step qRT-PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing


Posted on August 10, 2023
How do I measure histone modification?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingDNA and RNA QuantitationProtein


Posted on August 10, 2023
What are the advantages of native ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNA and RNA Quantitation


Posted on August 10, 2023
What are the disadvantages of Cross-linking ChIP?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDNA and RNA Quantitation


Posted on August 10, 2023
What is the basic principle behind RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 10, 2023
How does RNA-Seq contribute to the understanding of gene expression patterns?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What types of RNA can be analyzed using RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What sequencing technologies are commonly used for RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
How much sequencing depth is required for RNA-seq experiments?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What method can I use to remove ribosomal RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What are the different types of RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What are the advantages of long-read sequencing?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What main parameters should I consider when setting up a new RNA-seq experiment?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 9, 2023
How can I access and analyze a publicly available RNA-seq database?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
How do I prepare an RNA sequencing library?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are the challenges of RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
Why is RNA-seq considered superior to microarrays?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are some challenges and solutions in single cell RNA-Seq data analysis?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are the key steps involved in RNA-Seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)


Posted on August 4, 2023
What are the generally used detection methods in real time PCR (qPCR)?
Antibodies and ProteomicsAntibody and Protein LabelingDNA and RNA QuantitationPCR Detection of Viral DNA/RNAReal-Time PCR (qPCR)


Posted on August 3, 2023
What are the types and mechanisms of traditional vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What is removed during mRNA processing?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on August 3, 2023
How is transcribed mRNA processed in eukaryotes?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on August 3, 2023
How is mRNA related to DNA?
Antibodies and ProteomicsDNA and RNA QuantitationEnzymesNucleic AcidsNucleus


Posted on August 3, 2023
What is the significance of mRNA degradation?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the pathways of mRNA degradation?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
How are mRNA vaccines different from traditional vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
Can mRNA alter DNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
Besides vaccines, what other applications does mRNA have?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the pros of using mRNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the cons of using mRNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What is the difference between an mRNA and a viral vector vaccine?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What is the difference between a DNA and RNA vaccine?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the pros of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are cons of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What is the mechanism of DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the similarities between mRNA and DNA vaccines?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the similarities between RNA and mRNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
What are the differences between RNA and mRNA?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
Why is the mRNA not equal in length to the DNA it was transcribed from?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 3, 2023
How does an mRNA get into the nuclear pore?
Antibodies and ProteomicsDNA and RNA QuantitationNucleic AcidsNucleus


Posted on July 28, 2023
Where is chromatin found?
Cell Cycle AnalysisCell Cycle AssaysCellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
What are the types of chromatin?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 28, 2023
Which type of chromatin is generally more active?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusChromatin Immunoprecipitation (ChIP)


Posted on July 10, 2023
What is the significance of synthesis of aminoacyl-tRNAs?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAProteinamino acid sequence


Posted on July 10, 2023
What are the similarities between transfection and transduction?
Chemical ReagentsDNA and RNA QuantitationNucleic AcidsTransfectionTransfection Reagents


Posted on July 10, 2023
What factors affect transfection efficiency?
Chemical reagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the applications of transfection?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the steps for transfection with calcium phosphate?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the different types of transfection?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What factors should I consider when I choose transfection reagents?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What factors should I consider when I optimize the efficacy of my calcium phosphate transient transfection?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the differences between transfection and transduction?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the differences between generalized and specialized transduction?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagents


Posted on July 10, 2023
What are the steps involved in specialized transduction?
Chemical ReagentsDNA and RNA QuantitationTransfectionTransfection Reagentsbacteria


Posted on July 10, 2023
How does protein synthesis occur in prokaryotes?
DNA and RNA QuantitationNucleusPhysiological ProbesProtein to Protein Conjugation


Posted on July 10, 2023
How does protein synthesis occur in eukaryotes?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Protein


Posted on July 10, 2023
What is the role of nucleotides in protein synthesis?
Cell SignalingCellular ProcessesDNA and RNA QuantitationDNA and RNAProteinamino acid sequence


Posted on June 12, 2023
How are DNA sequences used to make proteins?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What is DNA made of?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What are the differences between B-DNA and Z-DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What type of DNA is found in humans?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
How is Z-DNA different from other forms of DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What are the differences between mitochondrial DNA and nuclear DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What is the structure of DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
Where is DNA found in cells?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on June 12, 2023
What are the different types of DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on June 12, 2023
What are the properties of DNA?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on May 20, 2023
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on May 20, 2023
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing


Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 2, 2023
What factors should I consider before I use restriction enzymes to cut DNA into small fragments?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on May 2, 2023
How does the cell protect its own DNA from disassembly?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleus


Posted on May 2, 2023
What are the two ways to label DNA molecules?
BioconjugationCellular Structures and OrganellesChemical ReagentsDNADNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on April 27, 2023
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium


Posted on April 27, 2023
What happens if I add too little MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAEnzymesPCR primerPolymerase Chain Reaction (PCR)


Posted on April 27, 2023
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on April 19, 2023
What is the mechanism of endonuclease?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleic Acids


Posted on April 19, 2023
How does a promoter affect DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleic Acids


Posted on April 19, 2023
What is the role of a promoter?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic Acids


Posted on April 19, 2023
What are the similarities between DNA and cDNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What is the basic structure of a gene?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusTransfectionTransfection Reagentsgene sequencing


Posted on April 19, 2023
Where are alleles located?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the functions of genes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusgene sequencing


Posted on April 19, 2023
What are the functions of alleles?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between genes and alleles?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between gene and DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
Is junk DNA completely useless?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What does DNA do?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on April 19, 2023
What are the differences between the sugar in DNA and the sugar in RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on March 1, 2023
What is the end product of transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the differences between cDNA and genomic DNA library?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the significance of cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the workflow of cDNA library construction?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the advantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the disadvantages of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the steps to construct a genomic DNA library?
DNA and RNA QuantitationDNA and RNAEnzymesGenomic DNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the applications of cDNA library?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on March 1, 2023
Where does the transcription terminate?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)PolymerasesReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
Are enhancers necessary for transcription?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the promoter sequences?
DNA and RNA QuantitationDNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)


Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor


Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What are the similarities between CDS and cDNA?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 23, 2023
Where is reverse transcriptase found?
DNA and RNA QuantitationDNA and RNAE. coliEnzymesbacteria


Posted on February 23, 2023
What are the differences between CDS and cDNA?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
How do I control and regulate gene expression?
DNA and RNA QuantitationDNA and RNA


Posted on February 23, 2023
What are the applications for reverse transcriptase?
DNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)Reverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What is the function of reverse transcriptase?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
How does reverse transcriptase work?
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What are the different technologies available to analyze transcription factors?
DNA and RNA QuantitationDNA and RNAWestern Blot


Posted on February 23, 2023
What are the methods used to measure and detect transcription?
DNA and RNA QuantitationDNA and RNANucleusRNAReal-Time PCR (qPCR)in situ hybridizationqPCR


Posted on February 23, 2023
What are the major enzymes used in DNA transcription?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNAEnzymesNucleusPolymerases


Posted on February 22, 2023
What are the similarities in the transcription between eukaryotes and prokaryotes?
Cell Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the differences in the transcription between eukaryotes and prokaryotes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What is the function of promoters?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the functions of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 22, 2023
What are the types of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 22, 2023
What is the structure of transcription factors?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on February 22, 2023
Where are genes transcribed?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleuscytoplasm


Posted on February 22, 2023
Are all transcribed genes translated into proteins?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusProtein


Posted on February 20, 2023
What are the differences between mRNA, rRNA, and tRNA?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusRNA


Posted on February 20, 2023
How does mRNA, tRNA and rRNA work together?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What are the two different types of rRNAs?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where does the rRNA function in cells?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where is rRNA located?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What is the basic structure of RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What is the structural difference between ribose and deoxyribose?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
Where is mRNA synthesized?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
How does mRNA work?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 20, 2023
What happens during the synthesis of mRNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusRNA


Posted on February 17, 2023
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on February 17, 2023
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 14, 2023
What happens if DNA replication does not occur?
Cell Cycle AnalysisCell Cycle AssaysDNA and RNA QuantitationDNA and RNA


Posted on February 14, 2023
What is the process of DNA replication?
Cell Cycle AnalysisCell Cycle AssaysDNA and RNA QuantitationDNA and RNAPolymerases


Posted on February 14, 2023
How many types of RNA are formed during transcription?
DNA and RNA QuantitationDNA and RNARNA


Posted on February 13, 2023
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What are the advantages of PCR (polymerase chain reaction)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What are the common PCR additives?
BSADMSODNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)bovine serum albumindetergentmagnesium


Posted on February 13, 2023
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How do I find the concentration of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What primer concentration should I use for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)


Posted on February 2, 2023
What is the structure of ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Why are ribosomes often referred to as the cell factories?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Where are ribosomes made?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Do ribosomes contain DNA?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the differences between 70s and 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the similarities between 70s ribosomes and 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the subunits of 70s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the subunits of 80s ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What does "S" refer to in a 70s ribosome and 80s ribosome?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the binding sites on ribosomes?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
How do eukaryotic ribosome binding sites work?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the differences between Shine Dalgarno and Kozak Sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What are the similarities between Shine Dalgarno and Kozak Sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
Where is the shine dalgarno sequence found?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on February 2, 2023
What is the significance of the shine dalgarno sequence?
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation


Posted on January 30, 2023
How do bacterial ribosome binding sites work?
Cell Structures and OraganellesDNA and RNA QuantitationNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 25, 2023
What are the general principles of plasmid isolation?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 23, 2023
What are the types of DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
How do acetylation and phosphorylation affect DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What is a histone made of?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What are the major functions of histones in the cell?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What is the order of DNA packaging in eukaryotes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNA QuantitationNucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What role do histones play in DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What is the importance of DNA packaging?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
How is DNA packaged in a cell?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological Probes


Posted on January 23, 2023
What are the similarities between promoters and enhancers?
DNA and RNA QuantitationDNA and RNADNA sequencingPolymerase Chain Reaction (PCR)


Posted on January 20, 2023
What are the similarities between plasmid DNA and genomic DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the common types of plasmids?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the applications of plasmids?
Cellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the properties of a plasmid?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the methods for plasmid detection?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
What are the important features required for a plasmid vector?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 20, 2023
How do I increase plasmid yield?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNA


Posted on January 20, 2023
How is plasmid removed from DNA?
BioconjugationCellular ProcessesCellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleus


Posted on January 18, 2023
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on January 18, 2023
What are the steps of emulsion PCR?
DNA and RNA QuantitationDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 18, 2023
How can I avoid primer-dimer formation during PCR amplification?
DNA and RNA QuantitationDNA and RNAPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on January 16, 2023
What are the differences between chromosomes and chromatids?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
How many chromatids are required to form a duplicated chromosome?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the differences between autosomes and allosomes?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the similarities between autosomes and chromosomes?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What is the smallest human chromosome?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What is the largest human chromosome?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the types of chromosomal mutations?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the classifications of structural chromosomal mutations?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the types of chromosomal number mutations?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the advantages of chromosomal mutations?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the disadvantages of chromosomal mutations?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
Where are chromosomes found in the body?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
How do scientists study chromosomes?
Cellular Structures and OrganellesDNA and RNA QuantitationFISHNucleusPhysiological Probesin situ hybridization


Posted on January 16, 2023
How many chromosomes do humans have?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
How do chromosome abnormalities happen?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the differences between gene mutation and chromosome mutation?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the similarities between gene mutation and chromosome mutation?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the differences between plasmid DNA and genomic DNA?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 16, 2023
What are the similarities between plasmids and chromosomes?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 11, 2023
What are the differences between genes and chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What is the relationship between genes and chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What do chromosomes look like?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the differences between DNA and chromosome?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the major types of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the functions of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the differences between the prokaryotic and eukaryotic chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 11, 2023
What are the similarities between the prokaryotic and eukaryotic chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationPhysiological Probes


Posted on January 11, 2023
What are the main components of chromosomes?
Cellular Structures and OrganellesDNADNA and RNA QuantitationNucleusPhysiological Probes


Posted on January 6, 2023
What are the differences between eukaryotic transcription and prokaryotic transcription?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on December 5, 2022
What are the differences between exome sequencing and whole genome sequencing?
Cell Structures and OraganellesDNA and RNA QuantitationNucleusPhysiological ProbesSanger Sequencing


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 10, 2022
What is the significance of ATP?
BioconjugationCell Proliferation AssaysCellular ProcessesDNA and RNA QuantitationEnzymesPolymerases


Posted on October 22, 2022
Is RNA editing better than DNA editing?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on October 22, 2022
What are the similarities between RNA splicing and alternative splicing?
BioconjugationCell Structures and OrganellesDNA and RNA Quantitation


Posted on October 22, 2022
What are the major steps involved in RNA processing?
BioconjugationCell Structures and OrganellesDNA and RNA QuantitationDNA and RNA Quantitation


Posted on October 14, 2022
How does stress change a cell?
Cell Structures and OrganellesCellular ProcessesDNA and RNA QuantitationMitochondriaPhysiological Probes


Posted on October 14, 2022
What does acridine orange stain?
BioconjugationCell Cycle AssaysCell Structures and OrganellesCellular ProcessesDNA and RNA QuantitationNucleus


Posted on October 4, 2022
What is the main function of the TE buffer?
Buffer Preparations and RecipesDNA and RNA QuantitationPhysiological ProbesTools


Posted on October 4, 2022
Why is Tris used in the TE buffer?
ToolsBuffer Preparations and RecipesDNA and RNA QuantitationPhysiological Probes


Posted on September 26, 2022
What are the disadvantages of Nanodrop light spectrophotometer?
DNA and RNA QuantitationNucleusPhysiological ProbesFluorescence Measurements and Instruments


Posted on September 26, 2022
What's the difference between qubit and nanodrop?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusFluorescence Measurements and Instruments


Posted on September 26, 2022
What are the differences between qPCR and ddPCR?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on September 19, 2022
What are the differences between gel electrophoresis and SDS-PAGE?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
Is SDS-PAGE a type of gel electrophoresis?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What is the advantage of a discontinuous buffer system?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What is the advantage of treating protein samples with SDS and β-mercaptoethanol?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What is the purpose of preparing a master mix?
Cell Structures and OrganellesDNA and RNA QuantitationPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on September 19, 2022
What are the examples of gel electrophoresis?
Cell Structures and OraganellesDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on August 25, 2022
What are the differences between restriction enzyme type 1, 2 and 3?"
DNA and RNA QuantitationEnzymesGel ElectrophoresisPhysiological Probes


Posted on March 25, 2022
What are the differences between linear and circular DNA?
Cell Structures and OrganellesNucleusPhysiological ProbesDNA and RNA Quantitation


Posted on December 5, 2020
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 23, 2020
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on September 30, 2020
What is the best DNA extraction method for genomics applications?
DNADNA and RNA QuantitationDNA and RNADNA extractionPolymerase Chain Reaction (PCR)


Posted on September 25, 2020
How do I remove genomic DNA contamination from isolated plasmid DNA?
DNADNA and RNA QuantitationDNA PurityDNA and RNA


Posted on July 22, 2020
How does bridge amplification work in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by synthesis approach in next-generation sequencing (NGS)?
Cellular processesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by ligation approach in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is a reversible terminator used in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What types of reversible terminators are available for next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is the general procedure of Illumina sequencing?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does pyrosequencing work?
DNADNA and RNA QuantitationDNA and RNADNA sequencingEnzymesgene sequencing


Posted on July 22, 2020
What is third-generation sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on June 22, 2020
What is the difference between transcription and reverse transcription?
DNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What are the advantages of Taq polymerase in PCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of Taq polymerase?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of polymerases used in PCR?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What are the three stages of a PRC process?
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of PCR technology?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How does SYBR Green work in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
How do hydrolysis probes work in qPCR? probes work?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How do you design a fluorescent hydrolysis probe in qPCR?
DNA and RNA QuantitationDNA and RNAReal-Time PCR (qPCR)qPCR


Posted on June 22, 2020
What is the melting curve analysis in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 1, 2020
What is gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
What is the function of electrophoresis buffer?
DNA and RNA QuantitationDNA and RNAGel Electrophoresisbuffer


Posted on June 1, 2020
What is the difference between denaturing and non-denaturing (native) gels?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisNucleic AcidsPhysiological Probes


Posted on June 1, 2020
What affects the migration rate of nucleic acid in gel?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPhysiological Probesethidium bromide


Posted on June 1, 2020
Why aren’t SDS-PAGE gels horizontal?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresis


Posted on May 9, 2020
Is next-generation sequencing (NGS) better than Sanger sequencing?
DNA and RNA QuantitationDNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Can next-generation sequencing (NGS) be used for virus genomes?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)RNA


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 24, 2020
What is the difference between ethidium homodimer and dihydroethidium in terms of staining?
Peptide and Oligonucleotide LabelingCell ViabilityDNA and RNA QuantitationDNA and RNAFluorescenceNucleic AcidsPhysiological Probes


Posted on April 17, 2020
What is the difference between uses of Agarose gel electrophoresis and Polyacrylamide gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)polyacrylamide gelMacromoleculeNucleic AcidsPhysiological Probes


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)