logo
AAT Bioquest

FAQ

Posted on February 20, 2024
What are the advantages of SMRT sequencing?
DNA and RNA QuantitationDNA sequencingGene Expression Analysis & GenotypingNucleic AcidsNucleusSanger Sequencing


Posted on February 20, 2024
What are the disadvantages of SMRT sequencing?
DNA and RNA QuantitationDNA sequencingGene Expression Analysis & GenotypingNucleic AcidsNucleusSanger Sequencing


Posted on February 20, 2024
What are the components required to set up the chain termination PCR?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on February 20, 2024
What does each component in chain termination PCR do?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on February 20, 2024
What are the different techniques commonly used to amplify DNA fragments before sequencing?
DNA and RNA QuantitationDNA sequencingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Sanger Sequencing


Posted on May 2, 2023
What are features of next generation sequencing?
DNADNA and RNADNA sequencingNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on January 23, 2023
What are the similarities between promoters and enhancers?
DNA and RNA QuantitationDNA and RNADNA sequencingPolymerase Chain Reaction (PCR)


Posted on January 18, 2023
What are the steps of emulsion PCR?
DNA and RNA QuantitationDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on September 1, 2020
What is DdNTP?
DdNTPddTTPDNADNA sequencing


Posted on July 22, 2020
What is third-generation sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What methods are used in next-generation sequencing (NGS) to amplify templates?
DNADNA and RNADNA sequencingPolymerase Chain Reaction (PCR)gene sequencing


Posted on July 22, 2020
How does bridge amplification work in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by synthesis approach in next-generation sequencing (NGS)?
Cellular processesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by ligation approach in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is a reversible terminator used in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What types of reversible terminators are available for next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the major platforms for next generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is the general procedure of Illumina sequencing?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is pyrosequencing?
DNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does pyrosequencing work?
DNADNA and RNA QuantitationDNA and RNADNA sequencingEnzymesgene sequencing


Posted on July 22, 2020
What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the different kinds of RNAs and what do they do?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is nick translation?
DNADNA and RNADNA sequencingFISHPeptide and Oligonucleotidein situ hybridization


Posted on July 22, 2020
What is the difference between DNA damage and DNA mutation?
DNADNA and RNADNA sequencingRNAdsDNAssDNA


Posted on July 22, 2020
What is gene silencing?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What are the differences between miRNA and siRNA?
DNADNA and RNADNA sequencingNucleic AcidsRNA


Posted on July 22, 2020
What is the difference between CRISPRi and RNAi?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on July 22, 2020
What is difference between CRISPR interference and CRISPR activation?
DNADNA and RNADNA sequencingEnzymesRNAbacteria


Posted on June 22, 2020
What is dCas9? And what is its application?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How is CRISPR-Cas9 used in gene knockout and knock-in?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How does CRISPR-Cas9 edit genes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
How is Cas prevented from cutting its own CRISPR locus?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is the protospacer adjacent motif (PAM) in CRISPR-Cas9?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What are introns?
DNADNA and RNADNA sequencingNucleic AcidsProteinRNAgene sequencing


Posted on June 22, 2020
What is CRISPR?
DNADNA and RNADNA sequencingNucleic Acidsbacteria


Posted on June 22, 2020
What is the CRISPR-Cas system in prokaryotes?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is CRISPR-Cas9 technology?
DNADNA and RNADNA sequencingEnzymesbacteria


Posted on June 22, 2020
What is a single guide RNA?
DNADNA and RNADNA sequencingEnzymesRNA


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
Is next-generation sequencing (NGS) better than Sanger sequencing?
DNA and RNA QuantitationDNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the difference between next-generation (NGS) and high-throughput sequencing (HTS)?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
How long does next-generation sequencing (NGS) take?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is targeted next-generation sequencing (NGS)?
DNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)dna sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
How pure does DNA have to be for next-generation sequencing (NGS)?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencing


Posted on May 9, 2020
What is a next-generation sequencing (NGS) library?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencingqPCR


Posted on May 9, 2020
Can next-generation sequencing (NGS) sample preparation be automated?
DNA and RNADNA sequencingPhysiological Probesgene sequencing


Posted on May 9, 2020
What is next-generation sequencing (NGS)?
Chemical ReagentsDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)gene sequencing


Posted on April 17, 2020
What is the difference between uses of Agarose gel electrophoresis and Polyacrylamide gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)polyacrylamide gelMacromoleculeNucleic AcidsPhysiological Probes


Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on April 7, 2020
What rhodamine dye do you have for automated DNA sequencing and nucleotide labeling?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesDNA sequencingFluoresceinsRhodamines and Rhodamine DerivativesPhysiological Probes


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)