FAQ

Posted on December 1, 2020
What are the reasons for high background in ELISA?
ELISAAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)BioconjugationHorseradish Peroxidase (HRP) and Poly-HRP

Posted on October 14, 2020
Is cAMP a second messenger?
ELISAPhysiological Probes

Posted on September 17, 2020
How do I set up a Sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISA

Posted on April 24, 2020
Which method is better for measuring the amount of antibodies in a sample, ELISA or FACS?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)ELISAEnzymesPeptide and Oligonucleotide

Posted on April 24, 2020
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer

Posted on April 24, 2020
What is the difference between capture antibody and marker-antigen?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISAIHCImmunohistochemistry (IHC)

Posted on April 17, 2020
Which fluorescent substrate should I use for B-galactosidase system for microscopy?
β-galactosidaseEnzymesbeta-galactosidasefluorescence microscopeELISAEnzyme-Linked Immunosorbent Assay (ELISA)lac OperonlacZ

Posted on March 13, 2020
What is an Indirect ELISA?
ELISAprimary antibodysecondary antibodyCell AssaySignal Amplificationmicroplate reader

Posted on January 6, 2020
What is a Direct ELISA?
HRPTMBELISADirect ELISAcolorimetricprimary antibodyHRP-labeled primary antibody

Posted on December 6, 2019
How do you activate T cells?
Cell AssayELISAIHCT cellantibody labelingmicroplate

Posted on September 6, 2019
What controls do I need for ELISA?
AmpliteControlsConjugationELISAHRPIgGantibody labelingenzyme-labeled

Posted on June 12, 2019
Can I customize ELISA techniques for my experiment?
cell assayhorseradish peroxidaseCell Assayhumanlive cell assaymicroplatemicroplate readerready-to-useCustomizationELISA

Posted on June 12, 2019
Are there different kinds of ELISA?
Cell AssayConjugationfluorescent plate readermicroplate readerELISADirect ELISA

Posted on April 2, 2019
What does my lab need to run ELISA?
Cell AssayELISADirect ELISAabsorbanceflow cytometerfluorescence microplate readermicroplate readerprimary antibody

Posted on April 2, 2019
How long does it take to get results from ELISA?
Cell AssayDirect ELISAELISA

Posted on April 2, 2019
What is a Competition ELISA?
ELISAprimary antibodysecondary antibodymicroplatemicroplate readerenzyme-labeledcell assayCell AssayCalculating Concentrationwavelength

Posted on March 28, 2019
What is a Sandwich ELISA?
ELISAprimary antibodyHRP-labeled primary antibodysecondary antibodySignal Amplificationmicroplatemicroplate readerConjugationhorseradish peroxidaseCalculating Concentration

Posted on March 23, 2019
Why should I use ELISA Testing?
cell assayhorseradish peroxidaseabsorbanceELISAmicroplate readerprimary antibodyDirect ELISACustomization

Posted on March 22, 2019
What kind of microplate should I use for ELISA?
wavelengthhorseradish peroxidasemolecular weightfluorescent microscopefluorescence microplate readermicroplate readermicroplateELISAexcitation wavelengthemission wavelengthready-to-useCustomizationAmplite

Posted on March 21, 2019
Do I have to use microplates for ELISA?
colorimetrichumanmicroplate readermicroplateELISAprimary antibodyready-to-usespectrophotometercentrifuge