FAQ

Posted on July 16, 2024
What are the types of cell fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on March 16, 2023
What is the main difference between preparing a sample for fluorescence microscopy versus light microscopy?
Cellular Structures and OrganellesFixationMicroscopy

Posted on March 9, 2023
What are the procedures of preparation of the biological specimens to be analyzed under a microscope?
FixationMicroscopyParaffin embedded sectionsfixed cells

Posted on March 9, 2023
What is the procedure for heat fixation?
FixationIHCImmunohistochemistry (IHC)Microscopybacteriafixed cells

Posted on December 8, 2022
What are the factors that will affect the rate and effectiveness of tissue fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the types of fixatives?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the general types of fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the commonly used chemical fixatives for microscopy?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the functions of fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the properties of an ideal fixative?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What are the differences between formalin and formaldehyde?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
Is formaldehyde a crosslinking agent?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
Why do tissues require fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on December 8, 2022
What is the purpose of cell fixation?
Chemical ReagentsBuffer Preparations and RecipesFixationfixed cells

Posted on April 24, 2020
Is it okay to stain cells for both intercellular and surface markers for FACS analysis?
Chemical ReagentsCell Cycle AnalysisCellular ProcessesCellular Structures and OrganellesFixationPermeabilizationPhysiological Probes

Posted on April 17, 2020
Which is the best conditions for methanol fixation of microtubules?
Bioconjugation Chemical ReagentsClassic DyesFixationPhysiological Probesfixed cells

Posted on April 17, 2020
What is the difference between PLL and PDL for surface coating?
Cell CultureFixationadherent cellsfixed cells Chemical Reagentsfluorescence microscope

Posted on April 17, 2020
How can I attach suspension cells to glass slides for fluorescence microscopy investigation?
FixationPBSfixed cellsfluorescence microscope Chemical Reagents

Posted on April 17, 2020
Techniques for storage of tissues post-fixation?
FixationStoragefixed cells Chemical ReagentsImmunohistochemistry (IHC)

Posted on April 17, 2020
What is the best fixation for fluorescence microscopy of GFP tagged proteins?
FixationPBSfixed cellsfluorescence microscope

Posted on February 25, 2020
What happens when you fix cells?
Fixation

Posted on January 4, 2020
What does rhodamine phalloidin do?
iFluor dyesActinF-actinFixationactin stainingactin polymerization inhibitorfluorescently labeled phalloidinphalloidin conjugatesphalloidin

Posted on December 11, 2019
What is Gram staining?
E. coliFixativesFixationbacteriaGram staining

Posted on December 11, 2019
Can I Gram stain living cells?
Cell AssayCell CytotoxicityFixationGram stainingbacteriafluorescence imaginglive cell assay

Posted on August 21, 2019
How do I prepare fixed cells for the labeling?
FixationfixableFixativescell-permeable probes

Posted on April 2, 2019
Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
Actin iFluor dyesCell AssayFixationF-actinFixativesfluorescence imagingfluorescence microscopephalloidin

Posted on March 22, 2018
What is the difference between formaldehyde and paraformaldehyde in cell fixation?
ActinF-actinFixationPhalloidin