FAQ

Posted on September 11, 2024
How do I remove cellular debris from my mixture?
Apoptosis and NecrosisCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)flow cytometry

Posted on July 16, 2024
What are the best methods to study apoptosis?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsGel ElectrophoresisMicroscopySpectral Flow Cytometry

Posted on June 25, 2024
What is wheat germ agglutinin (WGA)?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What's the structure of wheat germ agglutinin (WGA)?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What are the characteristics of wheat germ agglutinin (WGA)?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What are the applications of wheat germ agglutinin (WGA)?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What types of cells or tissues can be labeled with wheat germ agglutinin (WGA) marker?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What are the advantages of using wheat germ agglutinin (WGA) as a marker?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
How does wheat germ agglutinin (WGA) work as a marker?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
What forms are WGA available?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on June 25, 2024
How is WGA typically conjugated for us in experiments?
BioconjugationCellular Structures and OrganellesFlow Cytometry ReagentsPlasma MembraneSpectral Flow Cytometryfluorescence imaging

Posted on May 15, 2024
What are the limitations of spectral imaging technique?
Flow Cytometry ReagentsSpectral Flow Cytometry

Posted on May 15, 2024
What is spectral imaging?
Flow Cytometry ReagentsSpectral Flow Cytometry

Posted on April 10, 2024
What are the advantages of live cell imaging?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probeslive cell analysis

Posted on April 10, 2024
What are the limitations of live cell imaging?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probeslive cell analysis

Posted on April 10, 2024
What is image cytometry technique?
Chemical ReagentsCell Viability AssaysCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry

Posted on April 10, 2024
What is image cytometry technique used for?
Chemical ReagentsCell Viability AssaysCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probes

Posted on April 10, 2024
What are the advantages of image cytometry techniques?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probes

Posted on April 10, 2024
What are the advantages of flow cytometry?
Cellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry

Posted on April 10, 2024
What can I observe with image cytometry?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probes

Posted on April 10, 2024
What are the differences between image and flow cytometry?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probes

Posted on April 10, 2024
What is the principle of flow cytometry?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological Probes

Posted on April 10, 2024
What factors should I consider when designing intracellular flow experiments?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry

Posted on April 10, 2024
What are the applications of flow cytometry?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry

Posted on April 10, 2024
What is fluorescence-activated cell sorting (FACS)?
Cell SortingCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Physiological Probes

Posted on March 12, 2024
What are the applications of acridine orange stain?
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Nucleic AcidsNucleusOrganelle Staining

Posted on March 12, 2024
What is the principle of acridine orange stain?
Cellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleusOrganelle Staining

Posted on March 12, 2024
What are the advantages of acridine orange stain?
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleus

Posted on March 12, 2024
What are the limitations of acridine orange stain?
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleus

Posted on March 12, 2024
What are the chemical properties of acridine orange?
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleus

Posted on March 12, 2024
What is the general procedure of acridine orange staining?
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleus

Posted on February 2, 2024
Are there any reasons for using flow cytometry to measure calcium flux?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
Can I use the same calcium reagents for microscopy as I do for flow?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsMicroscopyPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
What does baseline fluorescence level represent for flow assays?
Flow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on February 2, 2024
Is it possible to measure real time calcium flux with a flow cytometer?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
Which calcium dyes work with flow cytometry lasers?
Calcium IndicatorsFlow Cytometry ReagentsFluoresceinsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
Can I combine Fura Red with Fluo-3 or Fluo-4 for ratiometric analysis?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
Should cells be equilibrated before a flow calcium assay?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
What kinds of stimulants are used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
What is the purpose of ionomycin in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
What buffers should be used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
What is the difference between negative control and baseline for flow cytometry calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on February 2, 2024
Are there any substitutes for probenecid in calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid

Posted on February 2, 2024
What is the purpose of probenecid in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid

Posted on February 2, 2024
Is a wash step required for calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry

Posted on January 16, 2024
What are the types of cell labeling?
FISHFlow Cytometry ReagentsMicroscopySpectral Flow Cytometryfluorescence microscopein situ hybridization

Posted on August 7, 2023
What are the advantages of allophycocyanin (APC)?
Antibodies and ProteomicsAntibody and Protein LabelingCellular PorcessesFlow Cytometry ReagentsPE and APC

Posted on August 7, 2023
What guidelines should I follow for successful tandem dye usage?
Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingFlow Cytometry ReagentsPE and APC

Posted on July 24, 2023
How is propidium iodide typically applied to cells?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysFlow Cytometry ReagentsImmunohistochemistry (IHC)Nucleic AcidsPropidium iodide (PI)Spectral Flow Cytometry

Posted on June 21, 2023
What are the principles of 5-CFDA-AM assay?
Chemical ReagentsCell Viability AssaysFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry

Posted on June 21, 2023
What is the principle of flow cytometric cell viability assays?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsLive or Dead

Posted on June 21, 2023
What are the limitations of fluorometric cell viability assays?
Apoptosis and NecrosisCell SignalingCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsLive or Dead

Posted on June 9, 2023
What are the methods used to analyze cell death?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesDNAFlow Cytometry ReagentsPropidium iodide (PI)Spectral Flow Cytometrycell death

Posted on June 9, 2023
What are cyclins and cyclin-dependent kinases, and how do they interact?
Cell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesEnzymesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Protein

Posted on June 8, 2023
What is the purpose of checkpoints in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
How do growth factors contribute to the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What is the major cell cycle control point in eukaryotes?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
How does a flow cytometer determine which part of the cell cycle a cell is in?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What are cyclins? What role do they play in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What are CDKs? What role do they play in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What is the mechanism of cyclin action?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What is the G0 part of the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
Where are the G1 checkpoints located?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What are the two types of regulators in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What is the importance of cell cycle control?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
When does DNA synthesis happen?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesDNAFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
How do the checkpoints of the cell cycle work?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
Which checkpoint of the cell cycle is considered the "point of no return"?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
Why do cells enter G0?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
Is the restriction point a checkpoint?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What controls the cell cycle at key checkpoints?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
How many checkpoints are there in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What is the significance of checkpoints in cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on June 8, 2023
What are checkpoints in the cell cycle?
Bioconjugation Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular ProcessesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)Immunohistochemistry (IHC)

Posted on May 24, 2023
What types of cells can I sort?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
What are the factors that affect the actual yield of the sorted cell samples?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
What effect will the cell sorting process have on my cells' viability?
Chemical ReagentsCell ViabilityFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
What are the techniques of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryCell Sorting Fluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
What is the significance of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
How are cells separated?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
What are the applications of cell sorting?
Chemical ReagentsFISHFlow Cytometry ReagentsFluorescenceImmunohistochemistry (IHC)Spectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
How do I prepare the samples for cell separation?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometryCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
Why should I use dead cell exclusion dye with my cell sorting experiment?
Flow Cytometry ReagentsFluorescenceLive or DeadSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
What are the general categories of cell sorting techniques?
Flow Cytometry ReagentsFluorescenceSpectral Flow CytometrycentrifugeCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on May 10, 2023
What are the applications of dextran-FITC conjugation?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsMicroscopySpectral Flow Cytometrydextranlive cell analysislive cell tracking

Posted on May 9, 2023
What advantages does FACS have over MACS?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on May 9, 2023
How do I choose the best cell separation method for my research?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on May 9, 2023
What are the key measures of performance for cell separation methods?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on May 9, 2023
What are the relationships between FACS and flow cytometry?
BioconjugationChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the applications of flow cytometry and FACS?
BioconjugationApoptosisCell Cycle AnalysisCell Proliferation AssaysChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
How does cell sorting work?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the differences between magnetic-activated cell sorting and FACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the pros of MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the two types of magnetic cell separation?
Flow Cytometry ReagentsFluoresceinsFluorescenceSpectral Flow Cytometry

Posted on May 9, 2023
What are the mechanisms used to label cells in MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry

Posted on January 26, 2023
How do I solve the dead cell issue?
Antibodies and Proteomics BioconjugationCell Viability AssaysFlow Cytometry ReagentsLive or DeadSpectral Flow Cytometry

Posted on January 26, 2023
How does the fixed cell viability staining dyes work?
Cell Viability AssaysCellular ProcessesDNA and RNAFlow Cytometry ReagentsLive or DeadProteinfixable

Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry

Posted on November 10, 2022
How do I dissolve a FITC-dextran?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry

Posted on November 10, 2022
Is FITC soluble in water?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry

Posted on November 10, 2022
What does FITC bind to?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization

Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence

Posted on November 7, 2022
What color does 7-AAD fluoresce?
Cell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsNucleusSpectral Flow Cytometryflow cytometry

Posted on September 17, 2022
What are the differences between viable and nonviable cells?
Cell Proliferation AssaysCell Viability AssaysCellular ProcessesFlow Cytometry Reagents

Posted on September 1, 2022
What is flow cytometry used for?
BioconjugationCell Cycle AssaysCell Viability AssaysCellular ProcessesFlow Cytometry Reagents

Posted on September 1, 2022
What are the different types of light used in a flow cytometry experiment?
Cell Cycle AssaysCell Viability AssaysCellular ProcessesFlow Cytometry Reagents

Posted on September 1, 2022
What are the similarities between flow cytometry and immunohistochemistry?
BioconjugationCell Cycle AssaysCell Structures and OrganellesCellular ProcessesFlow Cytometry ReagentsImmunohistochemistry (IHC)

Posted on September 1, 2022
What are the differences between flow cytometry and FACS?
BioconjugationCell Structures and OrganellesCellular ProcessesClassic DyesFlow Cytometry Reagents

Posted on September 1, 2022
What are the Similarities Between Flow Cytometry and FACS?
BioconjugationCell Structures a nd OrganellesCellular ProcessesFlow Cytometry Reagents

Posted on August 31, 2022
What are the factors to consider when choosing a crosslinking reagent?
BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsProtein to Protein Conjugation

Posted on August 11, 2022
What are the B cell surface markers?
Cluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsPhysiological ProbesClassic Dyes

Posted on August 5, 2022
What are the differences between flow cytometry and immunohistochemistry?
Flow Cytometry ReagentsImmunohistochemistry (IHC)BioconjugationAntibodies and ProteomicsProtein to Protein Conjugation

Posted on August 4, 2022
How does flow cytometry work?
Flow Cytometry ReagentsCell CytotoxicityPhysiological Probes

Posted on August 3, 2022
What light source is used in most flow cytometers?
Cell CytotoxicityFlow Cytometry ReagentsCellular ProcessesCell MetabolismPhysiological Probes

Posted on August 3, 2022
What are the commonly used fluorescent dyes in flow cytometry?
Cell MetabolismFlow Cytometry ReagentsCellular ProcessesCell Structures and OrganellesPhysiological Probes

Posted on July 12, 2022
What is the alternative to propidium iodide for dead cell staining?
Cell CytotoxicityCellular ProcessesBioconjugationFlow Cytometry ReagentsCell Cycle AssaysDNA and RNA Quantitation

Posted on July 8, 2022
What is the significance of CD markers on cells?
Flow Cytometry ReagentsAntibody LabelingAntibodies and ProteomicsPhysiological Probes

Posted on July 8, 2022
Is CD45 a cell surface marker?
Flow Cytometry ReagentsAntibody LabelingAntibodies and ProteomicsPhysiological Probes

Posted on July 8, 2022
What cell has CD45?
Physiological ProbesAntibody LabelingAntibodies and ProteomicsFlow Cytometry Reagents

Posted on July 8, 2022
Where is CD45 found?
Flow Cytometry ReagentsAntibodies and ProteomicsAntibody LabelingPhysiological Probes

Posted on May 24, 2022
How does 7-AAD bind to DNA?
Flow Cytometry ReagentsCell ViabilityCell Cycle AssaysCell Cytotoxicity

Posted on May 24, 2022
Is 7-AAD cell-permeable?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents

Posted on May 24, 2022
Can 7-AAD be fixed?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents

Posted on December 21, 2021
What is 7-AAD used for?
Cell ViabilityCell Cycle AssaysCellular ProcessesFlow Cytometry Reagents

Posted on December 21, 2021
What is 7-AAD?
DNA and RNA QuantitationCell ViabilityCell Cycle AssaysCellular ProcessesFlow Cytometry Reagents

Posted on December 10, 2021
Does 7-AAD stain necrotic cells?
Cell ViabilityCell Cycle AssaysCell CytotoxicityCellular ProcessesFlow Cytometry Reagents

Posted on December 9, 2021
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and NecrosisFlow Cytometry Reagents

Posted on December 9, 2021
Why do I need to use Annexin V Binding Buffer?
Cell ViabilityCell CytotoxicityApoptosis and NecrosisFlow Cytometry Reagents

Posted on November 15, 2021
What instruments are necessary to perform spectral flow cytometry?
Spectral Flow CytometryBioconjugationFlow Cytometry Reagents

Posted on November 15, 2021
What is spectral unmixing?
BioconjugationFlow Cytometry Reagents

Posted on November 11, 2021
Does spectral flow cytometry require signal subtraction?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
Who invented spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
What is the difference between conventional and spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on November 11, 2021
What is full spectrum flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on June 22, 2020
What are the three main components of a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
Why does the sample stream in flow cytometer need to be aligned in single file?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometer

Posted on June 22, 2020
How is the sample stream focused in a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What optics are used on a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometer lasersflow cytometry

Posted on June 22, 2020
What is spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What is the main difference between spectral flow cytometry and conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What is the difference between prisms and diffraction gratings in terms of light dispersion when they are used in spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What is limiting the applications of far-red and near-infrared fluorophores in conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
Why spectral flow cytometry is advantageous over conventional flow cytometry in multi-color experiments?
Flow Cytometry ReagentsFluorescencePE and APCSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is the mitosis phase in a cell cycle and what are the phases of mitosis phase?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
How long is the duration of each phase in a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
How does BrdU labeling work?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is fluorescence compensation in flow cytometry?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
How can I set up compensation for tandem dyes in flow cytometry?
Förster Resonance Energy Transfer (FRET)FRETFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What are the common methods for bacteria counting?
Flow Cytometry ReagentsFluorescenceGram Stainingbacteriaflow cytometryfluorescence imagingfluorescence microscope

Posted on June 1, 2020
What is cell division and types?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
What are cell cycle and cell cycle phases?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on May 18, 2020
What is FSC and SSC in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
Why does trypan blue quench FITC or GFP in flow cytometry?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What dyes are used in flow cytometry for cell viability testing?
Cell ViabilityCell Viability AssaysCell-Impermeable DNA dyesFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometrycell-impermeableflow cytometry

Posted on May 18, 2020
What is cell sorting in flow cytometry?
Antibodies and ProteomicsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is fluorescent activated cell sorting (FACS)?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
How is RNA isolated?
Antibodies and ProteomicsDNA and RNAExtractionFlow Cytometry ReagentsPhysiological ProbesPurificationRNASpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is the function of chloroform in RNA extraction?
Chemical ReagentsCellular Structures and OrganellesDNA and RNAFlow Cytometry ReagentsPurificationRNASpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is the difference between Side Scatter Pulse Width (SSC-w) and Side Scatter Pulse Height (SSC-h)?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometrymultiplex

Posted on May 9, 2020
What stain should I use to analyze apoptosis in flow cytometry?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesFlow Cytometry ReagentsFluoresceinsannexin Vflow cytometry

Posted on May 9, 2020
What markers can I use for investigating microglial activation in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)flow cytometry

Posted on May 9, 2020
Can mitochondrial tracking probes be used with flow cytometry?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsMitochondriaSpectral Flow Cytometryflow cytometrymitochondria membrane potential

Posted on May 9, 2020
What are the best methods to identify necrotic cells?
Antibodies and ProteomicsApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesDehydrogenaseEnzymesFlow Cytometry Reagentsflow cytometry

Posted on May 9, 2020
What does elevated fluorescence of PI alone in Annexin V/PI staining indicate?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysFlow Cytometry Reagentsannexin V

Posted on May 9, 2020
Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsImmunohistochemistry (IHC)PE and APCSpectral Flow Cytometry

Posted on May 9, 2020
What antibody concentration is recommended for intracellular staining using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingFlow Cytometry ReagentsIntracellular ProbesSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex

Posted on May 9, 2020
How can I analyze natural killer cells using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)Spectral Flow Cytometryflow cytometry

Posted on April 24, 2020
What is the right DAPI concentration to use in a flow cytometry experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry

Posted on April 17, 2020
Why should a cell die or undergo apoptosis?
ApoptosisApoptosis and NecrosisCell ViabilityCellular Processescell deathFlow Cytometry ReagentsPhysiological Probesflow cytometry

Posted on April 17, 2020
What is the role of sodium azide in flow cytometry?
Antibodies and Proteomics Chemical ReagentsFlow Cytometry ReagentsFluorescencePhysiological ProbesSpectral Flow Cytometry

Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent