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AAT Bioquest

Posted on February 2, 2024
What does baseline fluorescence level represent for flow assays?
Flow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on January 16, 2024
What are the advantages of fluorescent fusion proteins?
Antibodies and ProteomicsAntibody and Protein LabelingFluorescence


Posted on January 16, 2024
What are the disadvantages of fluorescent fusion proteins?
Antibodies and ProteomicsAntibody and Protein LabelingFluorescence


Posted on January 16, 2024
What is the purpose of cell labeling?
Antibodies and ProteomicsAntibody and Protein LabelingFluorescence


Posted on January 16, 2024
What are the common cell labeling methods?
Antibodies and ProteomicsFluorescenceGram StainingGram-negativeGram-positive


Posted on January 16, 2024
How can cells be visualized?
Antibodies and ProteomicsCellular Structures and OrganellesDNA and RNA QuantitationFluorescenceGram StainingGram-negativeGram-positive


Posted on January 16, 2024
What are the advantages of using fluorescent dyes to label cells?
BioconjugationCellular Structures and OrganellesClassic DyesFluoresceinsFluorescence


Posted on December 7, 2023
What is super-resolution microscopy?
FluorescenceFluorescence Measurements and InstrumentsMicroscopyfluorescence microscope


Posted on May 24, 2023
What are the common methods available to detect kinase activities?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA) Förster Resonance Energy Transfer (FRET)EnzymesFluorescenceProteinReal-Time PCR (qPCR)qPCR


Posted on May 24, 2023
What types of cells can I sort?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the factors that affect the actual yield of the sorted cell samples?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the techniques of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryCell Sorting Fluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What is the significance of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
How are cells separated?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What are the applications of cell sorting?
Chemical ReagentsFISHFlow Cytometry ReagentsFluorescenceImmunohistochemistry (IHC)Spectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
How do I prepare the samples for cell separation?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
Why should I use dead cell exclusion dye with my cell sorting experiment?
Flow Cytometry ReagentsFluorescenceLive or DeadSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What are the general categories of cell sorting techniques?
Flow Cytometry ReagentsFluorescenceSpectral Flow CytometrycentrifugeCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 9, 2023
How do I detect intracellular ATP levels?
BioconjugationADPATPCell Proliferation AssaysCell SignalingCellular ProcessesEnergyEnzymesFluorescencePhysiological Probesluminescense


Posted on May 9, 2023
What are the relationships between FACS and flow cytometry?
BioconjugationChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the applications of flow cytometry and FACS?
BioconjugationApoptosisCell Cycle AnalysisCell Proliferation AssaysChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
How does cell sorting work?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the differences between magnetic-activated cell sorting and FACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the pros of MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the two types of magnetic cell separation?
Flow Cytometry ReagentsFluoresceinsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the mechanisms used to label cells in MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on April 13, 2023
What types of samples can be used with an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How should an antifade reagent be chosen for a specific experiment or analysis?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
Can antifade reagents be used in other types of imaging besides fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How long does the fluorescence signal last when using an antifade mount medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
How do I reduce sample exposure in order to minimize photobleaching?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common anti-fading techniques?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What are some common components of an antifade mounting medium?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
What causes fading in fluorescence microscopy?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightnessfluorescence microscope


Posted on April 13, 2023
Can antifade mounting medium be used with all types of fluorophores?
BioconjugationFluorescenceImmunohistochemistry (IHC)anti-fadingfluorescence brightness


Posted on April 6, 2023
Are there any special considerations when choosing a mounting medium for specific applications?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
How do anti-fading agents work?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
Are there any disadvantages of using mounting medium in biology?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What is the purpose of a mounting medium in biology?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What are the different types of mounting medium used in biology?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
Can photobleaching be reversed?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What types of samples can be studied using anti-fading?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What are the advantages of using mounting medium in biology?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
How do I choose the right mounting media for my sample?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
How is a mounting medium applied to a microscope slide?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
How is mounting medium used in microscopy?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What are some antifading agents used to prevent photobleaching?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
When do I use photobleaching on purpose?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What strategies can I use to reduce photobleaching in live-cell imaging?
Fluorescenceanti-fadingfluorescence brightness


Posted on April 6, 2023
What causes photobleaching?
Fluorescenceanti-fadingfluorescence brightness


Posted on March 22, 2023
What are the applications of Ziehl-Neelsen staining?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the reagents used in the Ziehl-Neelsen stain?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the principle of the Kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the procedures of the kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the limitations of the Kinyoun-cold method?
FluorescenceGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What's the role of iodine in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the role of alcohol in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the fundamental differences between Ziehl-Neelsen technique and the Kinyoun technique?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the types of chemical reagents used in the staining process of the tissues?
Cellular Structures and OrganellesFluorescenceImmunohistochemistry (IHC)Organelle Stainingfluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What is the composition of the cell envelope?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
How do I distinguish gram negative and gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-negative cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-positive bacteria cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Is it easier to kill gram-positive bacteria than gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Which is more harmful, gram-positive bacteria or gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
Where are gram-positive bacteria found?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What is the significance of gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on January 13, 2023
What are the differences between chemiluminescence and electrochemiluminescence?
D-LuciferinEnzymesFluorescenceLuciferaseschemiluminescencefluorescence imagingluminescense


Posted on January 13, 2023
What are the differences between bioluminescence and fluorescence?
Bioconjugation Chemical ReagentsEnzymesFluorescenceLuciferasefluorescence imagingluminescense


Posted on January 13, 2023
What are the types of molecules that exhibit fluorescence?
Bioconjugation Chemical ReagentsCoumarinsCyaninesFluoresceinsFluorescenceRhodamines and Rhodamine Derivativesfluorescence imaging


Posted on January 13, 2023
What should I consider when choosing a fluorescence spectrometer?
Chemical ReagentsFluorescenceSpectral Flow Cytometryemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the different types of spectrometers used in fluorometry?
Bioconjugation Chemical ReagentsFluorescenceemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the differences between absorbance and fluorescence?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 12, 2023
What are the differences between fluorescence and luminescence?
Cell SignalingCellular ProcessesEnzymesFluorescencePhysiological Probeschemiluminescenceluminescense


Posted on January 12, 2023
What are the examples of fluorescent substances?
EnzymesFluorescencePhysiological Probeschemiluminescenceluminescense


Posted on January 12, 2023
What are the factors that cause fluorescence quenching?
Bioconjugation Förster Resonance Energy Transfer (FRET)Chemical ReagentsFluorescencePhysiological ProbesTide Quencher Dyes


Posted on December 15, 2022
What is Laser Induced Fluorescence?
Fluorescence


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 10, 2022
What does FITC bind to?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization


Posted on August 19, 2022
What are the steps of fluorescence spectroscopy?
FQYFluorescenceFluorescence Quantum Yield


Posted on August 19, 2022
What are the uses of fluorescence spectroscopy?
BioconjugationAntibody and Protein LabelingClassic DyesFluorescence


Posted on August 3, 2021
Do you have any dyes that can stain the nuclei in live cells? Are they also fixable and can I use them to stain fixed cells?
Nuclei StainingDNAFluorescenceemission wavelengthfixablefixed cellsfluorescence imaginglive cell analysisnuclear DNAMicroscopy


Posted on April 30, 2021
What is photoluminescence used for?
EnzymesFluorescencechemiluminescenceluminescense


Posted on July 22, 2020
What is the difference between fusion-signal FISH and split-signal FISH?
FISHFluorescencein situ hybridization


Posted on July 22, 2020
What methods are commonly used to label the probes in fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePeptide and OligonucleotidePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 22, 2020
What is fluorescence in situ hybridization (FISH)?
DNADNA and RNAFISHFluorescenceNucleic AcidsRNAfluorescence microscopein situ hybridization


Posted on July 22, 2020
What are the general steps of fluorescence in situ hybridization (FISH) experiments?
DNADNA and RNAFISHFluorescencePolymerase Chain Reaction (PCR)RNAin situ hybridizationssDNA


Posted on July 22, 2020
What types of probes are used in fluorescence in situ hybridization (FISH)?
DNA and RNAFISHFluorescencein situ hybridization


Posted on July 22, 2020
How do you prepare probes for fluorescence in situ hybridization (FISH)?
DNA and RNAEnzymesFISHFluorescencePolymerase Chain Reaction (PCR)in situ hybridization


Posted on July 15, 2020
6-ROX is predominately used as a reference dye for performing PCR detections, however, 6-ROX is very unstable compared to other rhodamine dyes. What is a better dye replacement?
Chemical ReagentsDNA and RNADNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Why spectral flow cytometry is advantageous over conventional flow cytometry in multi-color experiments?
Flow Cytometry ReagentsFluorescencePE and APCSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What is the difference between compensation and spectral unmixing?
FluorescenceSpectral Flow Cytometryflow cytometer


Posted on June 22, 2020
How does spectral flow cytometry manage autofluorescence?
FluorescenceSpectral Flow Cytometryautofluorescenceflow cytometry


Posted on June 1, 2020
What is the difference between chemiluminescence and fluorescence?
FluorescenceLuciferasechemiluminescencefluorescence imagingluciferin


Posted on June 1, 2020
Why is fluorescence more sensitive than UV-VIS absorption spectroscopy?
Chemical ReagentsAbsorbanceFluorescenceMeasure absorbancespectrophotometer


Posted on June 1, 2020
Why do some absorbing compounds fluoresce but others do not?
Chemical reagentsClassic DyesFluorescencePhysiological Probes


Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is fluorescence compensation in flow cytometry?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the difference between bacteria and viruses?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What are acid-fast bacteria?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
Are acid-fast bacteria gram positive or gram negative?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What are the common methods for bacteria counting?
Flow Cytometry ReagentsFluorescenceGram Stainingbacteriaflow cytometryfluorescence imagingfluorescence microscope


Posted on May 18, 2020
Are there upgraded trypan blue derivatives for cell viability testing?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesFluoresceinsFluorescencePhysiological Probes


Posted on May 18, 2020
What dyes are used in flow cytometry for cell viability testing?
Cell ViabilityCell Viability AssaysCell-Impermeable DNA dyesFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometrycell-impermeableflow cytometry


Posted on May 9, 2020
What phagocytic particles are best for assaying phagocytic activity of macrophages?
BioconjugationConjugationE. coliFluorescenceIntracellular pHPhysiological Probeslatex beadmultiplexphagocytosis


Posted on May 9, 2020
Is it possible to use DAPI and PI simultaneously?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingDAPIFluorescenceImmunohistochemistry (IHC)Propidium iodide (PI)


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 24, 2020
Can antibodies for flow cytometry be used for fluorescence microscopy?
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope


Posted on April 24, 2020
What is the difference between ethidium homodimer and dihydroethidium in terms of staining?
Peptide and Oligonucleotide LabelingCell ViabilityDNA and RNA QuantitationDNA and RNAFluorescenceNucleic AcidsPhysiological Probes


Posted on April 17, 2020
How to quench glutaraldehyde fluorescence?
BioconjugationFluorescenceautofluorescence Antibodies and ProteomicsAntibody and Protein LabelingPhysiological Probes


Posted on April 17, 2020
What is the role of sodium azide in flow cytometry?
Antibodies and Proteomics Chemical ReagentsFlow Cytometry ReagentsFluorescencePhysiological ProbesSpectral Flow Cytometry


Posted on February 4, 2020
What methods are currently used to quantify DNA?
DNA quantificationAbsorbanceFluorescenceAgarose gel electrophoresis


Posted on December 12, 2019
What is Stokes Shift?
Stokes ShiftFluorescenceemission wavelengthexcitation wavelengthfluorescence imaging