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AAT Bioquest

Posted on November 18, 2023
Can RNA and DNA ladders be used interchangeably?
DNA and RNA QuantitationGel ElectrophoresisPCR Detection of Viral DNA/RNADNA & RNA Markers and Ladders


Posted on August 23, 2023
Is gel electrophoresis a type of chromatography?
Chromatographic techniquesDNA and RNA QuantitationGel Electrophoresis


Posted on August 23, 2023
What are the differences between chromatography and electrophoresis?
Chromatographic techniquesDNA and RNA QuantitationGel Electrophoresis


Posted on August 21, 2023
What is a 50 bp DNA ladder?
Gel Electrophoresisdna ladder


Posted on August 14, 2023
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 2, 2023
Which technique is used to visualize DNA following a PCR reaction?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Proteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How do DNA fragments move through the gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What is the significance of running an uncut DNA on electrophoresis gel?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
When should I use 1kb DNA ladder?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleuspolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder visualized in gel electrophoresis?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide geldna ladder


Posted on May 2, 2023
How is a DNA ladder used in gel electrophoresis?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRpolyacrylamide geldna ladder


Posted on May 2, 2023
What are the limitations of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of post-electrophoresis staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsProteinnucleic acidpolyacrylamide gel


Posted on May 2, 2023
What are the limitations of in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What should be considered when I do in-gel staining?
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel


Posted on May 2, 2023
What are the advantages of in-gel staining?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gelprotein


Posted on April 26, 2023
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on April 26, 2023
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on September 29, 2022
How do I make a Tris buffer?
Gel ElectrophoresisPhysiological ProbesTools


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
What are the similarities between gel electrophoresis and SDS-PAGE?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What are the differences between gel electrophoresis and SDS-PAGE?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on September 19, 2022
Is SDS-PAGE a type of gel electrophoresis?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
How does an SDS PAGE gel work?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What is discontinuous gel electrophoresis?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleus


Posted on September 19, 2022
What is the advantage of a discontinuous buffer system?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What is the advantage of running a discontinuous protein gel rather than a continuous gel?
Gel ElectrophoresisPhysiological Probes


Posted on September 19, 2022
What is the advantage of treating protein samples with SDS and β-mercaptoethanol?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on September 19, 2022
What are the examples of gel electrophoresis?
Cell Structures and OraganellesDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on August 25, 2022
What are the similarities between restriction enzyme type 1, 2 and 3?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological Probes


Posted on August 25, 2022
What are the major differences between prokaryotic and eukaryotic gene expression?
DNA and RNA QuantitationGel ElectrophoresisNucleusPhysiological ProbesTransfection Reagents


Posted on August 25, 2022
What are the differences between restriction enzyme type 1, 2 and 3?"
DNA and RNA QuantitationEnzymesGel ElectrophoresisPhysiological Probes


Posted on March 8, 2022
What technique can I use to visualize (directly observe) the fragments of DNA?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 28, 2022
What is the process of RNA sequencing?
DNA and RNA QuantitationPhysiological ProbesGel ElectrophoresisNucleusCell Structures and Organelles


Posted on February 28, 2022
What is RNA sequencing?
Cell Structures and OrganellesPhysiological ProbesDNA and RNA QuantitationGel Electrophoresis


Posted on February 28, 2022
How do I do reverse transcriptase PCR?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 18, 2022
Are DNA fragments positive or negative?
DNA and RNA QuantitationGel ElectrophoresisPhysiological ProbesCell Structures and Organelles


Posted on February 10, 2022
What are the commonly used discontinuous gel buffer systems?
Gel ElectrophoresisPhysiological ProbesBioconjugationBuffer Preparations and Recipes


Posted on February 10, 2022
What are the applications of gel electrophoresis?
Cell Structures and organellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 10, 2022
What is the difference between native and denaturing gel electrophoresis?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 9, 2022
What are the basic types of electrophoresis?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 9, 2022
What are the steps of the gel electrophoresis process?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on February 9, 2022
What is Gel Electrophoresis Used For?
Physiological ProbesDNA and RNA QuantitationGel ElectrophoresisNucleusCell Structures and Organelles


Posted on February 4, 2022
What are the most common types of buffer solutions used in gel electrophoresis?
Gel ElectrophoresisCell Structures and OrganellesNucleusDNA and RNA QuantitationPhysiological Probes


Posted on January 14, 2022
Can ethidium bromide cause developmental defects?
Cell Structures and OrganellesNucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on January 14, 2022
What wavelengths of light does ethidium bromide absorb?
NucleusDNA and RNA QuantitaionPhysiological ProbesGel Electrophoresis


Posted on January 14, 2022
How should ethidium bromide be neutralized?
NucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on January 14, 2022
Is ethidium bromide teratogenic?
Cell Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisPhysiological ProbesNucleus


Posted on January 14, 2022
What is the difference between ethidium bromide and SYBR Safe?
Physiological ProbesDNA and RNA QuantitationNucleusCell Structures and OrganellesGel Electrophoresis


Posted on January 14, 2022
Can ethidium bromide be absorbed through skin?
Physiological ProbesGel ElectrophoresisNucleusDNA and RNA Quantitation


Posted on January 14, 2022
Is ethidium bromide radioactive?
NucleusDNA and RNA QuantitationGel ElectrophoresisPhysiological Probes


Posted on December 13, 2021
What is the mobile phase in high performance liquid chromatography (HPLC)?
Intracellular IonsPhysiological ProbesGel Electrophoresis


Posted on December 3, 2021
How should ethidium bromide be stored?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on December 2, 2021
What is the charge of ethidium bromide?
Gel ElectrophoresisNucleusPhysiological ProbesDNA and RNA Quantitation


Posted on December 2, 2021
Is ethidium bromide toxic?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on December 2, 2021
Are there safer alternatives to ethidium bromide?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on December 2, 2021
What is the cheapest DNA stain?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on December 2, 2021
When should ethidium bromide be added to agarose?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on December 1, 2021
Is ethidium bromide visible under UV?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA QuantitationCell Structures and Organelles


Posted on November 23, 2021
Does ethidium bromide bind proteins?
NucleusGel ElectrophoresisDNA and RNA QuantitationPhysiological Probes


Posted on November 10, 2021
How does ethidium bromide work?
NucleusGel ElectrophoresisPhysiological ProbesDNA and RNA Quantitation


Posted on August 18, 2021
Does silver stain stain DNA?
Gel ElectrophoresisDNA and RNA QuantitationCell Structures and OrganellesNucleus


Posted on August 18, 2021
What is the mechanism of silver staining?
Gel ElectrophoresisDNA and RNA QuantitationPhysiological ProbesCell Structures and Organelles


Posted on August 17, 2021
What are the advantages of silver staining?
Gel ElectrophoresisDNA and RNA QuantitationPhysiological ProbesCell Structures and Organelles


Posted on August 17, 2021
Is it possible to silver stain after Coomassie?
Gel ElectrophoresisPhysiological Probes


Posted on August 17, 2021
What are the best ways to dispose of silver stain?
DNA and RNA QuantitationGel Electrophoresis


Posted on August 16, 2021
Is silver stain toxic?
Gel ElectrophoresisDNA and RNA QuantitationCell Structures and Organelles


Posted on August 16, 2021
What does silver stain stain?
NucleusDNA and RNA QuantitationCell Structures and OrganellesGel Electrophoresis


Posted on August 16, 2021
Can SYPRO Ruby be reused?
Gel ElectrophoresisDNA and RNA QuantitationNucleus


Posted on August 16, 2021
How does SYPRO Ruby work?
Gel ElectrophoresisDNA and RNA QuantitationCell Structures and OrganellesPhysiological Probes


Posted on July 29, 2021
Do Ionic detergents denature proteins?
Gel ElectrophoresisCell Structures and organellesPhysiological Probes


Posted on July 28, 2021
What makes a detergent ionic?
Gel ElectrophoresisDNA and RNA QuantitationPhysiological ProbesCell Structures and Organelles


Posted on April 19, 2021
What makes up the chromosomes of cells?
DNA and RNAGel ElectrophoresisNucleus


Posted on June 1, 2020
What is the difference between nitrocellulose and PVDF membranes in western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
Why aren’t SDS-PAGE gels horizontal?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresis


Posted on June 1, 2020
What is gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA and RNAGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
What is the function of electrophoresis buffer?
DNA and RNA QuantitationDNA and RNAGel Electrophoresisbuffer


Posted on June 1, 2020
What is the difference between denaturing and non-denaturing (native) gels?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisNucleic AcidsPhysiological Probes


Posted on June 1, 2020
What affects the migration rate of nucleic acid in gel?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPhysiological Probesethidium bromide


Posted on June 1, 2020
What is the difference between PAGE and SDS-PAGE?
Buffer Preparations and RecipesGel ElectrophoresisGel Electroporesisbufferdetergent


Posted on June 1, 2020
What is the difference between stacking gel and resolving gel in SDS-PAGE?
Agarose gel electrophoresisGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
How does a stacking gel work?
Antibodies and ProteomicsAgarose gel electrophoresisGel Electrophoresispolyacrylamide gel


Posted on June 1, 2020
What is the difference between SDS-PAGE and western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
Why are proteins transferred from gel to a membrane in western blot?
Gel ElectrophoresisProtein PurificationWestern Blot


Posted on May 18, 2020
What does EDTA do in agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisChelatorEDTAGel Electrophoresisbuffer


Posted on April 24, 2020
What can I do if low molecular weight proteins are not showing any bands in western blot?
Antibodies and ProteomicsAntibody and Protein LabelingGel ElectrophoresisProteinWestern Blot


Posted on April 24, 2020
What is the difference between western blotting and immunoprecipitation?
Antibodies and ProteomicsGel ElectrophoresisProteinWestern Blot


Posted on April 24, 2020
What is an appropriate incubation time for primary antibody in western blot analysis?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel


Posted on April 24, 2020
What is the difference between western blotting and far-western blotting?
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingEnzymesGel ElectrophoresisProteinagarose gelpolyacrylamide gel


Posted on April 24, 2020
What is the major difference between Northern and Southern blotting?
Antibodies and ProteomicsAgarose gel electrophoresisDNA and RNAEnzymesGel ElectrophoresisNucleic AcidsProteinWestern Blotpolyacrylamide gel


Posted on April 17, 2020
What is the difference between uses of Agarose gel electrophoresis and Polyacrylamide gel electrophoresis?
Agarose gel electrophoresisDNA and RNA QuantitationDNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)polyacrylamide gelMacromoleculeNucleic AcidsPhysiological Probes


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)