AAT Bioquest


Posted on June 1, 2020
What is enhanced chemiluminescence?
D-LuciferinHRPHorseradish Peroxidase (HRP) and Poly-HRPLuciferaseWestern Blotchemiluminescenceluciferin

Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation

Posted on April 24, 2020
Why is TMB used in conjunction with HRP in ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHRPHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)

Posted on January 6, 2020
What is a Direct ELISA?
HRPTMBELISADirect ELISAcolorimetricprimary antibodyHRP-labeled primary antibody

Posted on September 6, 2019
What controls do I need for ELISA?
AmpliteControlsConjugationELISAHRPIgGantibody labelingenzyme-labeled

Posted on August 19, 2019
What is catalase and how does it work in H2O2-based assays?

Posted on April 2, 2019
Why should you use ABTS when measuring the concentration of an antioxidant in a biological sample?

Posted on April 2, 2019
Does EDTA interfere with your Cell Meter™ Colorimetric Antioxidant Activity Assay Kit (Cat # 15900)?
AbsorbanceH2O2HRPABTSCell Assay

Posted on June 25, 2018
What is the molecular weight (mw) of horseradish peroxidase (HRP)?
HRPTMBABTSADHPH2O2molecular weighthydrogen peroxidehorseradish peroxidase