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AAT Bioquest

Posted on June 24, 2024
What are examples of chemiluminescent substrates?
Antibodies and Proteomics Chemical ReagentsEnzymesHorseradish Peroxidase (HRP) and Poly-HRPLuciferasesPhysiological Probeschemiluminescence


Posted on May 8, 2024
What are the common chromogens used in immunohistochemistry (IHC)?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingFluorescenceHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)


Posted on May 2, 2024
Which enzyme is most often used in blotting procedures?
Antibodies and ProteomicsEnzymesHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)Secondary Antibodies


Posted on May 2, 2024
Why is horseradish peroxidase the enzyme that is used most often in blotting procedures?
Antibodies and ProteomicsEnzymesHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)Secondary Antibodies


Posted on November 16, 2023
What are the common enzymes used as labels for ELISA assays?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)EnzymesHorseradish Peroxidase (HRP) and Poly-HRP


Posted on November 7, 2023
What are the most commonly used enzymes for protein detection?
Enzyme-Linked Immunosorbent Assay (ELISA)Biotin and StreptavidinHorseradish Peroxidase (HRP) and Poly-HRPWestern BlotProtein Assays & Analysis


Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot


Posted on October 8, 2021
Can I use azide in ELISA buffers?
Enzyme-Linked Immunosorbent Assay (ELISA)Horseradish Peroxidase (HRP) and Poly-HRPEnzymes


Posted on September 27, 2021
How do I know if an antibody is primary or secondary?
EnzymesSecondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 27, 2021
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics


Posted on September 22, 2021
How do I choose secondary antibodies?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPEnzyme-Linked Immunosorbent Assay (ELISA)


Posted on September 22, 2021
What is HRP conjugated secondary antibody?
EnzymesSecondary AntibodiesHorseradish Peroxidase (HRP) and Poly-HRPPower Styramide™ Signal Amplification (PSA™)


Posted on September 22, 2021
Why is an HRP conjugated secondary antibody used in western blotting?
Horseradish Peroxidase (HRP) and Poly-HRPEnzymesSecondary AntibodiesAntibodies and Proteomics


Posted on September 21, 2021
What does HRP bind to?
BioconjugationAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPProtein to Protein Conjugation


Posted on September 21, 2021
What do anti-human antibodies do?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 21, 2021
What is anti human IgG?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on August 18, 2021
What does methylene blue indicate?
BioconjugationHorseradish Peroxidase (HRP) and Poly-HRPEnzymes


Posted on December 1, 2020
What are the reasons for high background in ELISA?
ELISAAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)BioconjugationHorseradish Peroxidase (HRP) and Poly-HRP


Posted on June 1, 2020
What is enhanced chemiluminescence?
D-LuciferinHRPHorseradish Peroxidase (HRP) and Poly-HRPLuciferaseWestern Blotchemiluminescenceluciferin


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation


Posted on April 24, 2020
Why is TMB used in conjunction with HRP in ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHRPHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)